Xylem
...#62;> The Joint Genome Institute (JGI) has
shotgun sequence d the genome s of two strain s of the fastidious, xylem -limited bacteria , Xylella fastidiosa, to high draft (eightfold coverage). Full article >>> pits -- Thin regions of the cell wall in xylem conducting cells . T...
Subcloning
... a new cloning vector that allows double adaptor
shotgun subcloning of large target molecule s into pUC-18. Full article >>> One would use phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are ones commonly used in cloning and/or ...
Shotgun sequencing
...onal Human Genome Research Institute, defines
shotgun sequencing and compares the two methods. related ...s. Full article >>> Whole genome
shotgun sequencing skips the mapping step of clone -based sequencing . Full article >>> ...
Shotgun method
...n of significant portion of phage sequence s in
shotgun libraries and the up front cost of library production. Full article >>>...
Shotgun cloning
... The sequencing of complex genome s requires
shotgun cloning (or subcloning ) of genomic DNA (or BACs) into vectors that carry smaller insert s and that can serve as template s in sequencing reactions. Full article >>>...
Genome
... mapping , and clone -based and whole genome
shotgun sequencing of smaller fragments. Full article >>> A genome is all of the genes in a particular organ ism. Bacteria or virus vectors can be used to store fragments of the DNA from a species . Full article >>> ...
Directional cloning
...ng region (MCR) has several unique sites for both
shotgun and directional cloning . Full article >>>...
Contig
... long contig uous DNA sequence assembled from
shotgun sequencing . Related ... Full article >>> A genetic map depicting the relative order of a linked ( contig uous) library of small overlapping clones representing a complete chromosomal segment . Full article >>> ...
Consensus sequence
...om multiple, imperfect examples. Multiple lanes of
shotgun sequence can be merged to show a consensus sequ...om multiple, imperfect examples. Multiple lanes of
shotgun sequence can me merged to show a consensus sequence . The optimal sequence of nucleotide s rec...
Concatemer
... form long concatemer s, and then generation of
shotgun libraries. Full article >>>...
Clone
...e -based sequencing (also known as hierarchical
shotgun sequencing ) the first step is mapping . One first constructs a map of the chromosomes , marking them at regular intervals of about 100 kilobase s (kb). Full article >>> ^ ORTHO CLONE OKT3 Information page at Ortho Biotech. Retriev...