Oligonucleotide in Biological Dictionary

Oligonucleotide

... An oligonucleotide is a short nucleic acid polymer, typically ... precursors. Automated ... oligonucleotide synthesis is the chemical synthesis of ... in current laboratory practice because ... oligonucleotide n. A short polymer of two to twenty nucleotides. ...

Allele-specific PCR

... An Allele Specific oligonucleotide (or ASO) is a short piece of synthetic DNA ... target DNA sequence, ... Allele specific oligonucleotide An Allele Specific oligonucleotide (or ASO ) is a short piece of ... to detect ...

Primer extension

... assay design, bio-rad bioplex Primer Extension . • Allele Specific oligonucleotide Ligation. • Sequencing ... One can label the primer and apply the ... DNA sequence is ... 2.4 Primer extension . 2.5 5'- nuclease. 2.6 oligonucleotide ligase assay ... Because, primer extension is based on the highly ...

CDNA array

... ... be transitioning from the nylon membrane cDNA arrays to oligonucleotide arrays ... Each spot on these arrays contains 10 nanograms of PCR ... bound with cDNA produced by reverse ... Software and methods for oligonucleotide and cDNA array data analysis ... The cDNA array experiment ...

Oligodeoxyribonucleotide

... online resource for all laboratory protocols ... Versatile synthesis of oligodeoxyribonucleotide –oligo spermine conjugates ... An antisense oligonucleotide phosphorothioate, previously shown to inhibit HIV-1 viral expression in chronically infected H9 cells, was fluorescently labeled to ... ... ...

Melting temperature

... STRUCTURE & MELTING TEMPERATURE . REPEATS, SECONDARY STRUCTURE ... Knowing the melting temperature of a fragment of DNA or of an oligonucleotide is ... Freezing and Melting | World of Earth Science. Freezing and Melting summary with 4 pages of encyclopedia ... a solid to a liquid as ...

Gene silencing

... a breakthrough in understanding the phenomenon of nucleolar dominance, the silencing of an entire parental set of ribosomal RNA genes in a ... oligonucleotide probes used to silence gene expression ... ProbeDB > Applications > Gene Silencing . PubMed. All ... Comparison of gene silencing ...

Gene expression

... expression information in MGI. ... Gene Expression Notebook (GEN) Mouse Phenome Database (MPD) Deltagen and Lexicon Knockout Mice ... long oligonucleotide arrays for gene expression analysis including tiling expression , mutiplex expression for a broad range of prokaryotic and eukaryotic genomes ...

DNA probes

... slides in triplicate or quadruplicate from a ... DNA probe An agent that binds directly to a predefined sequence of nucleic acids. ... What oligonucleotide probe would best hybridize with the DNA sequence 5' ... DNA fingerprint n. An individual's unique sequence of DNA base pairs, ...

CpG island

... site Cytosine Phosphate Guanine CpG sites are regions of DNA where a cytosine nucleotide occurs next ... CpG island . Allele specific oligonucleotide ... We have established a CpG - island -extraction algorithm, which we previously ... The CpG Island Searcher was applied to the latest ...

Antisense

... Online Dictionary with audio pronunciations, thesaurus, Word of the Day, and word games. Conclusion: The era of respirable antisense oligonucleotide (RASON) therapeutics was introduced in 1997 with the publication of preclinical data on a 21-mer ... The antisense technique could potentially ...