ligase

Splicing

...ctice, a pool of DNA fragments are treated with ligase (see " Ligase ") in the presence of ATP , and all possible splicing products are produced, ... Full article >>> Because of the complexity of the splicing process, one trans cribed RNA may be spliced in alternate ways to produc...

S1 nuclease

...repaired and ligated with T4 DNA polymerase and ligase . Full article >>> In these cases, although the m RNA is monocistronic , the nuclear transcript was polycistronic . S1 nuclease mapping of 5' ends of m RNA s produced by trans-splicing , unlike primer extension , does not l...

RNase

...g between the fragments is linked together by DNA ligase . Full article >>> cytoplasm (in eukaryotes m RNA is "processed" before being exported), where it is bound to ribosomes and trans lated into protein . After a certain amount of time the message degrades into its component n...

Restriction fragment

...leave can be reformed by other enzymes known as ligase s, so that restriction fragment s carved from different chromosomes or genes can be spliced together, provided their ends are complement ary (more below). Full article >>> A technique in which DNA restriction fragment length p...

Recombinant DNA molecule

... and then joining the fragments together with DNA ligase . Full article >>> Note that each recombinant DNA molecule includes a region where nucleotide s from one of the origin al molecule s are paired with nucleotide s from the other. But no matter. Full article >>> 11(b...

Recombinant DNA

...ble to cut DNA at specific sites. Together with ligase , which can join together fragments of DNA , restriction enzymes formed the initial basis of recombinant DNA ... Full article >>>...

Probe

...n be easily detected. Examples include Q-beta and ligase chain re action . A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ... Full article >>> Dr. Richard M organ , of the National Human Genome Research Institute's Clinical Gene Therapy Branch, defines probe . related terms: de...

Overhang

...ferent parent DNA molecule s, and the enzyme ligase will then covalently link the strand s. Full article >>> Homopolymer tailing Extension of the 3' ends of a piece of double- strand ed DNA using the enzyme terminal trans ferase and a single deoxy nucleoside triphosphate as sub...

Okazaki fragments

... DNA replication and are joined together by DNA ligase to produce a new DNA strand . Full article &#...last RNA attaches, and DNA polymerase and DNA ligase come along to convert the RNA (of the primer s) to DNA , and seal the gaps in between the Okaz...

Mitochondrial membrane

...he double helix near the replication fork DNA ligase - fills in nicks and gaps made in poly nucleotide strand s ... Full article >>> Cristae the convolutions of the inner mitochondrial membrane (crista = crest) Cruciferae plant family in which the flower s have four petal...

Ligation

...ated by a single- strand break. Catalyzed by DNA ligase . Full article >>> Ligation Possi...ction enzymes and provide the conditions for DNA ligase to do its work, the pieces of these plasmid s can rejoin (thanks to the complement arity of their...

Ligase

...ed ... Full article >>> Search for ligase in these other data bases too Definition of ligase : An enzyme that can rejoin a broken phosphodiester bond in a nucleic acid . Full article ...

Lagging strand

... DNA replication and are joined together by DNA ligase to produce a new DNA strand . Full article >>> Strand of DNA being replicated continuously. In DNA replication , the strand that is made in the 5' to 3' direction by continuous polymerization at the 3' growing tip. See also ...

Karyotype

...s arranged in order from largest to smallest. L ligase -- an enzyme that functions in DNA repair . Full article >>>...

Helicase

...he double helix near the replication fork DNA ligase - fills in nicks and gaps made in poly nucleotide strand s ... Full article >>> After the helicase unwinds the DNA , RNA primase is bound to the starting DNA site . Full article >>> Example helicase An en...

Enzyme

...e >>> Ligase : An enzyme , T4 DNA ligase , which can link pieces of DNA together. The pieces must have compatible ends (both of them blunt, or else mutually compatible sticky ends ), and the ligation re action requires ATP . Full article >>> inducible - enzyme not ...

Double helix

...he double helix near the replication fork DNA ligase - fills in nicks and gaps made in poly nucleotide strand s ... Full article >>> Domi nant gene : A gene which, when present on a chromosome , passes on a certain physical character istic, thereby dominating over a recessive ge...

DNA replication

...he double helix near the replication fork DNA ligase - fills in nicks and gaps made in poly nucleotide strand s ... Full article >>> width="400" valign="TOP" A linking enzyme essential for DNA replication ; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the...

DNA repair

...all the time. Full article >>> DNA ligase - protein that joins (ligates) DNA strand s;... DNA occurs e. Full article >>> L ligase -- an enzyme that functions in DNA repair . Linkage -- the greater association in inheritance...

DNA ligase

DNA ligase catalyzes the formation of the covalent bonds bet...chromosome ). Full article >>> DNA ligase The enzyme that joins the 5' and 3' ends of poly nucleotide chains by the formation of a phosp...

DNA gyrase

...>>> DNA gyrase : see gyrase . DNA ligase : Enzymatic activity responsible for creating phosphodiester bond s between the 5' end of one strand of DNA and the 3' end of another strand . Full article >>> DNA gyrase : catalyzes the negative supercoil ing of prokaryotic D...

DNA

...aling them. Full article >>> DNA ligase (ly-gaze) width="400" valign="TOP" A linking enz...ONUCLEASE . Full article >>> DNA ligase - protein that joins (ligates) DNA strand s; used by cells for DNA repair , by molecular ...

Cristae

...he double helix near the replication fork DNA ligase - fills in nicks and gaps made in poly nucleotide strand s ... Full article >>> cristae Structures formed by the folding of the inner membrane of a mitochondrion (sing.: crista). PICTURE ... Full article >>> Cri...

Compatible ends

...e >>> Ligase : An enzyme , T4 DNA ligase , which can link pieces of DNA together. The pieces must have compatible ends (both of them blunt, or else mutually compatible sticky ends ), and the ligation re action requires ATP . Full article >>>...

Blunt ends

...wo fragments may then be covalently bonded by DNA ligase . Blunt ends may be ligated, but the re action is significantly slower. These terms are most commonly used to describe the product of a restriction enzyme digestion of DNA . Full article >>> Blunt End : A terminus of a duplex ...

Allele

...fragment, and would be ligated in presence of DNA ligase ; ... Full article >>> Allele : Alternative form of a genetic locus ; a single allele for each locus is inherited separately from each parent (e.g., at a locus for eye color the allele might result in blue or brown eyes). Fu...