isolated

Ubiquitin

...proteins . Full article >>> We have isolated and character ized 5 additional imprinted genes from the 1MB-genomic intervals surrounding human and mouse PEG3, including Zim1 (imprinted Zinc-finger gene 1), Zim2, Zim3, Usp29 ( Ubiquitin -specific processing protease 29), and Znf264. Ful...

Transfection

... VH gene . RAG1 and RAG2 are purified proteins isolated from lymph oid tissue . Co- transfection of RAG1 and RAG2 ... Full article >>> Furthermore, we describe the development of a reliable transfection method for motor neuron s based on microinjection, which will be extremely useful ...

Transcriptase

... ( RNA -dependent DNA polymerase ). An enzyme isolated from retrovirus -infected cells that synthesizes a complement ary (c) DNA strand from an RNA template . RFLP . See Restriction -fragment-length polymorphism . Full article >>> Reverse transcriptase : An enzyme which w...

Taq polymerase

...merase which is very stable at high temperatures, isolated from the thermophilic bacterium Thermus aquaticus.... Taq polymerase . A heat-stable DNA polymerase isolated from the bacterium Therrnus aquaticus, used in PCR . (See Polymerase .) ... Full article >...

Synapsis

...T Taq polymerase . A heat-stable DNA polymerase isolated from the bacterium Therrnus aquaticus, used in PCR . Full article >>>...

Sympatric

...nge in the genome that produces a reproductively isolated sub population in the midst of its parent popula...curs when two subpopulations become reproductively isolated without first becoming geographically isolated. Insects that live on a single host plant provide...

Species

species reproductively isolated systems of breed ing population s having a simil... >>> A species is a reproductively isolated population that shares a common gene pool and a common niche . This definition defines a speci...

Speciation

...y begins when subpopulations of a species become isolated geographically (for example, by habitat fragment...are in reproductive contact but are reproductively isolated from all other population s ... Full article >>> Speciation Main article: Speciat...

Southern blotting

...es per genome . These additional sequence s were isolated by molecular cloning and character ized by sequencing . Full article >>> Southern blotting Trans fer by absorption of DNA fragments separated in electrophoretic gels to membrane filters (nylon or nitro cellulose ) for detec...

Southern blot

...es per genome . These additional sequence s were isolated by molecular cloning and character ized by sequencing . Full article >>> Southern blot ting Trans fer by absorption of DNA fragments separated in electrophoretic gels to membrane filters (nylon or nitro cellulose ) for det...

Sequencing

...#62;>> These additional sequence s were isolated by molecular cloning and character ized by sequencing . Full article >>> The clones are verified by sequencing . cDNA array is hybrid ized with Cy-dye labeled sample and identical control for the same set of experiments. Dat...

Screening

...zen or so agar plates to grow several ten thousand isolated colon ies. Full article >>> genetic screen ing The use of a number of mutant forms of a species to test for a specific type of change or deficit, as with respect to biological clocks. Full article >>> Genetic screen...

RT-PCR

...ls . Full article >>> Once RNA is isolated it can be reverse trans cribed back into DNA ( complementary DNA to be precise, known as c DNA ), at which point traditional PCR can be applied to amplify the gene , this methodology is called RT-PCR . Full article >>> Several ...

Reverse transcriptase

...e ( RNA -dependent DNA polymerase ). An enzyme isolated from retrovirus -infected cells that synthesizes a complement ary (c) DNA strand from an RNA template . RFLP . See Restriction -fragment-length polymorphism . Full article >>> Reverse transcriptase : An enzyme which wil...

Restriction enzymes

...Restriction enzymes : An endonuclease enzyme , isolated from bacteria , that recognizes specific base -pair sequence s within DNA and causes endonucleolytic cleavage of the DNA at a site determined by the recognized DNA sequence s. Full article >>> The use of restriction enzyme...

Restriction enzyme

...zymes (" restriction endonuclease s") generally isolated from bacteria , which are able to recognize and cut specific sequence s (" restriction site s") in DNA . Full article >>> Restriction enzyme cutting site : A specific nucleotide sequence of DNA at which a particular restri...

Restriction

...zymes (" restriction endonuclease s") generally isolated from bacteria , which are able to recognize and c...estriction enzymes : An endonuclease enzyme , isolated from bacteria , that recognizes specific base -pair sequence s within DNA and causes endonucleo...

Recombinant

... that also prevent PT pore opening in cells and isolated mitochondria . Full article >>>...

Proteins

...gically, ... Full article >>> First isolated from striated muscle and often referred to as one of the muscle proteins . G- Actin is the globular monomeric form of actin , 6.7 x 4.0nm: it polymerizes to form filamentous F- actin . Full article >>> This allows capture and is...

Prophase

...T Taq polymerase . A heat-stable DNA polymerase isolated from the bacterium Therrnus aquaticus, used in PCR . Full article >>>...

Probe

...obe a genomic library . A restriction fragment isolated from the end of the positive clones is used to re probe the genomic library for overlapping clones . Full article >>> : One of the more modern methods in cytogenetics , which uses fluorescence-labelled chromosome -specific DNA...

Primer extension

...art of an m RNA is. For example, perhaps you have isolated a cDNA clone , but you don't think that the clone has all of the 5' untranslated region . Full article >>> Singlesided primer extension with dideoxy nucleotide chain terminator s is avoided with the con sequence that the sequ...

Primer

...art of an m RNA is. For example, perhaps you have isolated a cDNA clone , but you don't think that the clone has all of the 5' untranslated region . Full article >>> RNA primer s - primer s used to synthesize DNA strand s by actin g as a template RNA splicing - process in which...

Population

...up of organ isms of the same species relatively isolated from other groups of the same species . See deme . Gamete ... Full article >>> Population s: Groups of similar individuals who tend to mate with each other in a limited geographic area. This can be as simple as a field of flower ...

Polyclonal antibodies

.... Antigen . Full article >>> Blood isolated from these animals contains polyclonal antibodies -- multiple antibodies that stick to the same antigen . Full article >>> poly many polyclonal antibodies A heterogeneous pool of antibodies produced in an animal by a numbe...

Plasmid

...A circular piece of DNA present in bacteria or isolated from bacteria . Full article >>> PLASMID - An extrachromosomal,usually circular, double- strand ed DNA which is capable of replication within a cell , and which usually contains and express es genes encoding resistance to ant...

Photobiology

...ology in June 2001, the collaborators showed that isolated PSI re action centers can photo-evolve hydrogen, ... Full article >>>...

Overlapping clones

...article >>> A restriction fragment isolated from the end of the positive clones is used to re probe the genomic library for overlapping clones . This process is repeated several times to walk across the chromosome and reach the gene of interest. Full article >>>...

Osteocyte

...ult bone, has developed from an osteoblast, and is isolated in a lacuna of the bone substance. osteon Unit of bone structure; Haversian system. Full article >>> osteocyte A cell located in lacunae of vertebrate bone, that deposits a matrix of collagen and calcium phosphate that forms a ...

Origin

... Ca++ ions to cross cell membrane s) origin ally isolated as an antibiotic from Streptomyces chartreusensis. Also known as Calcimycin, Antibiotic A23187. Full article >>> I began writing this book while employed by a major Midwestern company which was at the time venturing aggressively in...

Nuclein

...tance rich in phosphorus. In the 1870's, Miescher isolated a similar substance from salmon sperm . Full art... he called " nuclein " in 1869. Somewhat later, he isolated a pure sample of the material now known as DNA from the sperm of salmon, and in 1889 his pupil, ...

Northern hybridization

... RT-PCR and Northern hybridization using RNA isolated from appropriate breast cancer cell lines and primary tumor s. Full article >>>...

Mucosa

... Full article >>> Adult stem cell s isolated from the olfactory mucosa ( cells linking the inside of the nose involved in the sense of smell) have the ability to develop into many different cell types if they are given the right chemical environment . Full article >>> all...

Migration

...nt of individuals between otherwise reproductively isolated population s. Related Terms: Population ... Full article >>> Migration into or out of a population can breakdown genetic differences between population s. Mutation s developing in one population may be spread to other popul...

Microevolution

...reexisting one or the divergence of reproductively isolated population s into new species . Full article >>> Microevolution Main article: Microevolution Microevolution refers to small- scale changes in gene frequencies in a population over the course of a few generations. Full art...

Macromolecules

...er a frozen layer of suspension that contains many isolated macromolecules for ... Full article >>> In DNA sequencing gels, urea is included to denature ds DNA to ss DNA strand s. In denaturing gel s, macromolecules tend to be separated on the basis of size and (to some extent) ch...

Liposomes

... that also prevent PT pore opening in cells and isolated mitochondria . Full article >>>...

Lineage

...else we could not recognise it), is reproductively isolated (else the lineage would merge into others, ... Full article >>> A method to study cell lineage or function by selectively destroying a small number of cells . For example, a laser beam can be used to focus on and kill one cell ...

IS element

...ull article >>> This element was not isolated for many years after this due to its extreme reactivity - it is separated from its compound s only with difficulty and then it immediately attacks the remaining materials of the compound . Full article >>> Other organ isms, like an...

Inverse PCR

...STSs from the ends of selected cosmid s are being isolated by inverse PCR and being used to screen for additional cosmid s, verifying contig structure and screening YACs. It is estimated that 1. Full article >>>...

In vitro

...e same behavior or capability as dendritic cell s isolated ex vitro, but they are often used for research, as they are still much more readily available than genuine DCs. Full article >>> In vitro Outside a living organ ism. In a glass (test-tube). Antonym: in vivo. Cloning ... Full articl...

Immunoprecipitation

...cess whereby a particular protein of interest is isolated by the addition of a specific antibody , followed by centrifugation to pellet the resulting immune complexes. Full article >>> Such antibodies could be used in the high throughput genome wide study of gene product s by immunofluo...

Human

...icle >>> The human gene has been isolated and clone d using recombinant DNA techniques. This is leading to improved treatment. Royal Families of Europe ... Full article >>> The Evidence for Human Evolution Permission is given to copy and print these pages for non-profit...

Genomic library

...obe a genomic library . A restriction fragment isolated from the end of the positive clones is used to re probe the genomic library for overlapping clones . Full article >>> A technique of isolating clones from a genomic library that are not contig uous by skipping a region betwe...

Genetic drift

...ion and establishes a new one in a geographically isolated region. genome : the set of all genes that specify an organ ism's trait s. Full article >>> Genetic drift Main article: Genetic drift Genetic drift describes changes in gene frequency that cannot be ascribed to selective pre...

Gene library

... >>> The gene to be knocked out is isolated from a mouse gene library . Then a new DNA sequence is engineered which is very similar to the origin al gene and its immediate neighbor sequence , except that it is changed sufficiently to make it inoperable. Full article >>>...

Gel

...ncorporated to the same extent in stable complexes isolated by gel filtration, showing similar dimensions and stoichiometries. Full article >>> The current forces the molecule s through pores in a thin layer of gel , a firm jelly-like substance. The gel can be made so that its pores are j...

Fusion Protein

...ic intranuclear pattern in HeLa cell s and on the isolated nuclear matrix. Full article >>> Tamm is a professor of Biophysics at the University of Virginia where he is studying the structures and interactions of viral fusion protein s in lipid bilayers, mainly using the influenza surface ...

Enzymes

...striction enzymes : An endonuclease enzyme , isolated from bacteria , that recognizes specific base -pair sequence s within DNA and causes endonucleolytic cleavage of the DNA at a site determined by the recognized DNA sequence s. Full article >>> : A group of enzymes mainly ...

Endemism

Endemism can also develop in other biologically isolated areas, such as the highlands of Ethiopia or large bodies of water like Lake Baikal. [edit] Ecoregions with high endemism ... Full article >>> Endemism Presence within a localised area; peculiar to that area. Extant Still in exi...

DNA polymerase

...;>> Taq polymerase : A DNA polymerase isolated from the bacterium Thermophilis aquaticus and which is very stable to high temperatures. It is used in PCR procedures and high temperature sequencing . Full article >>> DNA polymerase . See Polymerase . DNA polymorphism . One o...

Deme

...up of organ isms of the same species relatively isolated from other groups of the same species . See deme . Gamete Mature male or female reproductive cell ( sperm or ovum ) with a haploid set of chromosomes (23 for human s). Full article >>>...

Complementary DNA

... ... Full article >>> Once RNA is isolated it can be reverse trans cribed back into DNA ( complementary DNA to be precise, known as c DNA ), at which point traditional PCR can be applied to amplify the gene , this methodology is called RT-PCR . Full article >>> The copy...

Community

...ctive community of population s (reproductively isolated from others) that occupies a specific niche in nature." ... Full article >>> Community : A group of population s of different species occupying a given place at a given time that are viewed as interdependent. An aggregation of in...

Colon

...zen or so agar plates to grow several ten thousand isolated colon ies. Full article >>> gonidia - reproductive cells of colon ial eukaryotes such as Volvox. goosecoid- a homeo domain transcription factor express ed in the dorsal lip of the blastopore ; may be a key factor is specif...

Cloning

...#62;>> These additional sequence s were isolated by molecular cloning and character ized by sequencing . Full article >>> " Cloning and Character ization of a Potassium-dependent Sodium/Calcium Exchanger in Drosophila ". J. Cell Biol. 147(3):659-669. P. Raghu, et al. (2000...

Cleavage

...Restriction enzymes : An endonuclease enzyme , isolated from bacteria , that recognizes specific base -pair sequence s within DNA and causes endonucleolytic cleavage of the DNA at a site determined by the recognized DNA sequence s. Full article >>> Post- translation al processi...

Class

...zymes (" restriction endonuclease s") generally isolated from bacteria , which are able to recognize and cut specific sequence s (" restriction site s") in DNA . Full article >>> Dicot yledones the Class (in Division Sperm atophyta; Sub division Angiosperm ae) of plant s that ha...

Cell membrane

... Ca++ ions to cross cell membrane s) origin ally isolated as an antibiotic from Streptomyces chartreusensis. Also known as Calcimycin, Antibiotic A23187. Full article >>> The material between the nuclear and cell membrane s; includes fluid ( cytosol ) organelles , and various membranes...

Carrier

... Ca++ ions to cross cell membrane s) origin ally isolated as an antibiotic from Streptomyces chartreusensis. Also known as Calcimycin, Antibiotic A23187. Full article >>> Carrier -- an individual heterozygous for a single recessive gene . c DNA -- complementary DNA produced from a...