hybridization

VNTR

... has supplanted RFLP by Southern blotting and hybridization in ana lysis of VNTR 's. Simple sequence repeat s can be used as genetic marker s. Details on many VNTR marker s used by the US FBI are available. Full article >>> Essentially, these VNTR 's are small repeats of DNA seque...

Tubulin

... microtubules ) and a reduction of the in situ hybridization signal for tubulin m RNA . Full article >>> During polymerization, both the α- and β- subunits of the tubulin dimer are bound to a molecule of GTP. The GTP bound to α- tubulin is stable, but the GTP bound to β- tubulin may b...

Tissue

...The c DNA common to all conditions is removed by hybridization with excess RNA from other cells (i.e. Full article >>> Parasitoid: An animal that feeds in or on another living animal , consuming all or most of its tissue s and eventually killing it. Parthenogenesis : Development of an in...

Stringency

...gher the stringency , lower the probability of hybridization . Increase the temperature or decreasing the salt ...62;> Stringency Conditions affecting the hybridization of nucleotide sequence s. Higher stringency conditions require more base pair ing between the...

Streptavidin

...idin . STRINGENCY - The conditions employed for hybridization which determine the specificity of the anneal in...gency : A term used to describe the conditions of hybridization . Full article >>> Biotin has an extraordinarily high affinity for streptavidin w...

Strand

...62;> Strand -specific fluorescence in situ hybridization ( FISH ) is a modification of the standard FISH method that allows hybridization of a single- strand ed deoxyoligomer probe to only one chromatid of a metaphase chromosome ....

Speciation

...ll article >>> Speciation through hybridization and/or polyploid y has long been considered much less important in animals than in plant s [[[refs.]]]. A number of reviews suggest that this view may be mistaken. Full article >>> Sympatric speciation happens when members o...

Southern blotting

...l article >>> Southern blotting A hybridization technique that enables researchers to determine t... 7). Southern blotting a type of nucleic acid hybridization test, in which single- strand ed DNA from two sources interact. Full article >>> ...

Southern blot

.... related terms: deoxyribonucleic acid ( DNA ), hybridization ... Full article >>> Southern bl...article >>> Southern blot ting A hybridization technique that enables researchers to determine the presence of certain nucleotide sequence s in...

Somatic cell

...e. Full article >>> Somatic cell hybridization In somatic cell hybridization , human cells and rodent tumor cells are fused ( hybrid ized); over time, after the chromos...

Solution hybridization

Solution hybridization : A method closely related to RNase protection (see " RNase protection assay"). Solution hybridization is designed to measure the levels of a specific m RNA species in a complex population of RNA ...

Somatic

... Full article >>> Somatic cell hybridization In somatic cell hybridization , human cells and rodent tumor cells are fused ( hybrid ized); over time, after the chromos...

Single locus probe

9 positives per marker by hybridization of 45 single locus probe s. All 1,076 marker -positive BAC clones were confirmed to be part of 45 single- marker contig s by restriction -fingerprinting. Full article >>> Probe s made of these sequence s are single locus pro...

Simple Sequence Repeat

... has supplanted RFLP by Southern blotting and hybridization in ana lysis of VNTR 's. Simple sequence repeat s can be used as genetic marker s. Details on many VNTR marker s used by the US FBI are available. Full article >>>...

Screening

...s. Full article >>> Screening by hybridization involves spreading the mixture of bacteria out on a dozen or so agar plates to grow several ten thousand isolated colon ies. Full article >>> genetic screen ing The use of a number of mutant forms of a species to test for a spec...

Ribonucleotides

... (usually radiolabeled) and used as a probe for hybridization reactions. Full article >>> Polymerase -- any enzyme that catalyzes the formation of DNA or RNA from deoxyribonucleotide s or ribonucleotides . Full article >>> deoxyribonucleotide s Nucleotide s containing ...

Ribonuclease

... (usually radiolabeled) and used as a probe for hybridization reactions. Full article >>> Ribonuclease : see " RNAse ". Ribonucleic acid ( RNA ): A chemical found in the nucleus and cytoplasm of cells ; it plays an important role in protein synthesis and other chemical activities of th...

RFLP

...ical RFLP ana lysis by Southern blotting and hybridization required the testing laboratory to have on hand DNA molecule s from which to make probe s. Delays and difficulties in the dissemination of these DNA s among laboratories occurred. Full article >>> Search for RFLP in these other...

Restriction enzyme

...articular combination of restriction enzyme and hybridization probe , the pattern of hybrid izing bands shown above was obtained. The length of the restriction fragment s recognized by the probe is dimorphic in this population . Full article >>> Restriction enzyme s cut DNA wherever ...

Prometaphase

...of chimera s was measured by fluorescent in situ hybridization ( FISH ) to human prometaphase spreads. Full article >>>...

Probe

...gene ... Full article >>> Probe hybridization . Joe Gray [University of California, San Francisc... to detect the complement ary base sequence by hybridization . Full article >>> sa probe [Gk. sapros, rotten, putrid + bios, life] An organ ism ...

Probability

...gher the stringency , lower the probability of hybridization . Increase the temperature or decreasing the salt concentration raises the stringency . Related Hybridization Melting temperature ... Full article >>> Mortality death rate or probability of dying at any given age (mort = death ...

Population

... and Southern blot ted. The blot was probe d by hybridization with a clone d fragment of the tree's genome . ...bdomen . Full article >>> Solution hybridization is designed to measure the levels of a specific m RNA species in a complex population of RNA ...

Polyploid

...cle >>> polyploid produced by the hybridization of two species . See amphi diploid . A B C D E F...ll article >>> Speciation through hybridization and/or polyploid y has long been considered much less important in animals than in plant s [[...

Polyp

...e >>> polyp loid produced by the hybridization of two species . See amphi diploid . A B C D E F...ll article >>> Speciation through hybridization and/or polyp loid y has long been considered much less important in animals than in plant s ...

Polymorphisms

...ull article >>> The marker s where hybridization occurred are referred to as RFLP s ( restriction -fragment-length polymorphisms ). The longer fragment in sickle- cell individuals is interpreted as evidence of a mutation in the recognition sequence . Full article >>> (two or m...

Plaque

...be examined for purity by plaque or by colony hybridization to total rodent and total human DNA . This information will be compared with purity data derived during chromosome sorting and library construction. Full article >>> Neurofibrillary tangles and amyloid plaque s are commonly fo...

Phylogeny

...tree of living hominoids is based on DNA - DNA hybridization data (the work of Charles Sibley and Jon Ahlquist...ersity). Link to a description of the DNA - DNA hybridization method. Full article >>> Molecular phylogeny is the use of a gene 's molecular ...

Phylogenetic tree

...age s on a phylogenetic tree , generally through hybridization or through lateral gene transfer . Fairly common in certain land plant clade s; reticulation is thought to be rare among metazoa ns. Full article >>> Statistical support for phylogenies Does phylogenetic inference find correct...

PCR

...ical RFLP ana lysis by Southern blotting and hybridization required the testing laboratory to have on hand DNA molecule s from which to make probe s. Full article >>> With PCR , it is routinely possible to amplify enough DNA from a single hair follicle for DNA typing . Some workers ha...

Package

... A software package developed for interpreting hybridization autorads from high-density grids, gridHyb is used to improve the quality of existing maps, and also in the distribution of Chromosome Library clones based on probe data. Full article >>> DNA is tightly package d into struct...

Oligonucleotide

...ochips have been tested as diagnostics of genetic hybridization diseases. Several methods have been evaluated for the preparation of hybridization probe s. Full article >>> Oligonucleotide A short sequence of poly nucleotide ...

Oligodeoxyribonucleotide

...s in length, which may be used as a primer or a hybridization probe . Oligodeoxyribonucleotide s are synthesized chemically under automated conditions. Full article >>> An in situ methodology employing solution conditions has been developed for binding oligodeoxyribonucleotide 'third- strand...

Nucleotide

...s in length, which may be used as a primer or a hybridization probe . Full article >>> Nucleotide . A building block of DNA and RNA , consisting of a nitrogenous base , a five-carbon sugar, and a phosphate group . Together, the nucleotide s form codon s, which when strung together form ...

Nucleic acid

..... Full article >>> Nucleic acid hybridization Formation of a double- strand ed molecule by ...2; Southern blotting a type of nucleic acid hybridization test, in which single- strand ed DNA from two sources interact. Strand s with similar nucleic a...

Northern hybridization

Northern hybridization . ( Northern blotting ). A procedure in which RNA...... Full article >>> (See Northern hybridization , Southern hybridization .) Hybridoma . A hybrid cell , composed of a B Iymphocyte fused to a ...

Northern blot

... cDNA library , deoxyribonucleic acid ( DNA ), hybridization , m RNA ... Full article >>> Nor...;>> Northern blot ting . See Northern hybridization . Northern hybridization . ( Northern blot ting ). A procedure in which RNA fragments are trans...

Northern blotting

...62;>> Northern blotting . See Northern hybridization . Northern hybridization . ( Northern blotting ). A procedure in which RNA fragments are trans ferred from an agarose gel...

Nontarget organism

...nded recipient. Northern blotting . See Northern hybridization . Northern hybridization . ( Northern blotting ). Full article >>>...

Microscope

... expression levels in the sample are detected by hybridization of the labeled RNA s to the probe s on the slide. Full article >>> They have about 1000 chromatid s; produced by DNA replication without mitosis . When viewed under a microscope , the many chromatid s look like a giant chromo...

Mapping

... (See Mapping .) Copy DNA . See c DNA . Cross- hybridization . The hydrogen bond ing of a single- strand ed DNA sequence that is partially but not entirely complement ary to a singlestranded substrate . Full article >>> Deletion mapping -- the use of overlapping deletion s to localiz...

Marker

... microtubules ) and a reduction of the in situ hybridization signal for tubulin m RNA . Full article >>> Gusella and his research team, who used RFLP s to identify a marker . Testing a large library of human DNA fragments, Gusella et al. found the needle in the haystack. The enzyme ...

Lysis

...ical RFLP ana lysis by Southern blotting and hybridization required the testing laboratory to have on hand DNA molecule s from which to make probe s. Delays and difficulties in the dissemination of these DNA s among laboratories occurred. Full article >>> *Owl Pellet Lab (ana lysis ques...

Localize

... incorporated into a detailed fluorescent in situ hybridization ( FISH ) order map. Full article >>> placode Localize d, plate-like thickening of vertebrate head ectoderm from which a specialized structure develops; such structures include eye lens , special sense organ s, and certain ne...

Ligase

...in poly nucleotide strand s DNA renaturation ( hybridization ) - process whereby two complement ary nucleic acid strand s form a double helix during an anneal ing period; ... Full article >>> DNA ligase - protein that joins (ligates) DNA strand s; used by cells for DNA repair ...

In situ hybridization

in situ hybridization locating a gene by adding specific radioactive ...on of the radioactivity on the chromosome after hybridization ... Full article >>> Thomas Ried defines fluorescence in situ hybridization ( FISH...

Hydrogen bond

... . Full article >>> nucleic acid hybridization - coming together ( anneal ling) of single- strand ed nucleic acid sequence s by hydrogen bond ing of complement ary bases to form double- strand ed molecule s; ... Full article >>> : Reversible disruption of hydrogen bond s ...

Hybridoma

...... Full article >>> (See Northern hybridization , Southern hybridization .) Hybridoma . A hybrid cell , composed of a B Iymphocyte fused to a tumor cell , which grows...

Hybridization

...emical on the chromosome or in the cell after hybridization . Full article >>> in situ hybridization locating a gene by adding specific radioactive probe s for the gene and detecting the location...

Heteroduplex Dna

...ence s to form a duplex molecule . (See Northern hybridization , Southern hybridization .) Hybridoma . Full article >>> heteroduplex DNA Duplex DNA containing compleme...

Genomic complexity

...A that are suitable for comparative fluorescence hybridization s with probe s of total mammalian genomic complexity . Full article >>> A recent information-theoretic (but intuitively evident) definition identifies genomic complexity with the amount of information a sequence stores about its ...

Genome

...n. (See Mapping .) Copy DNA . See c DNA . Cross- hybridization . Full article >>> Alu repetitive sequence -- the most common dispersed repeated DNA sequence in the human genome accounting for 5% of human DNA . The name is derived from the fact that these sequence s are cleaved by the ...

Gene transfer

... their very nature, phylogenetic tree s hide any hybridization and lateral gene transfer (Woese 2002) that m...age s on a phylogenetic tree , generally through hybridization or through lateral gene transfer . Fairly common in certain land plant clade s; reticulation ...

Gene therapy

...owth hormone . Southern blotting . See Southern hybridization . Full article >>> Germ cell ( germ line ) gene therapy . The repair or re- placement of a defective gene within the gamete -forming tissue s, which produces a heritable change in an organ ism's genetic constitution. GMO. Genet...

Gene families

...ilies were used to ex amine the extent of cross hybridization . Full article >>> Gene families are groups of genes related, but not necessarily identical, in structure. The members of the family can have functions that are identical or similar. Full article >>> Ontogeny Ont...

Gene flow

...me cases, between population s of two species ( hybridization ). Full article >>> Gene Flow Many species are made up of local population s whose members tend to breed within the group. Each local population can develop a gene pool distinct from that of other local population s. Full ...

Gene

...me cases, between population s of two species ( hybridization ). Full article >>> Gene : A biochemical unit of hereditary , often coding for an entire protein . Generalist: A pest or natural enemy that can utilize a wide range of species as host or prey. Full article >>> ...

Food web

...he phenotype might not be recoverable by future hybridization s -- remember, ... Full article >>> The fishery has yet to recover, and may not recover at all because of a possibly stable change in the food web . The North-west Atlantic population s spawn in the winter and spring in the Cape Cod ...

FISH

...;> Thomas Ried defines fluorescence in situ hybridization ( FISH ). illustrated: View illustration ... Ful...>>> FISH See fluorescence in situ hybridization . Related Terms: Fluorescence in situ hybridization ( FISH ) ... Full article >>> ...

Familial

...anean fever. related terms: chromosome , gene , hybridization , mapping ... Full article >>> F...n inherited trait . FISH (fluorescence in situ hybridization ): A physical map ping approach that uses fluorescein tags to detect hybridization of probe s wi...