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fragments in Biological Dictionary

Seed coat

... or soften the seed coat . ... Seed coat fragments that remain in lint after. the ginning process decrease ... seed coat fragments affect not ... yielding fragile seed coat ... in the experimental cotton was ... seed coat fragments concluded that lint cleaning was ... ginning ...

Restriction fragment

... restriction digest A unique family of DNA fragments produced by digestion of a given DNA molecule ... nucleotides in one or more of the restriction fragments and is ... A restriction fragment length ... ... the DNA of an organism is digested (cut into fragments ) with a restriction ... Restriction ...

Okazaki fragments

... during DNA replication. The lengths of Okazaki fragments are between 1,000 to 2,000 nucleotides long in ... long in ... Okazaki fragments DNA sequences, 100 to 200 nucleotides long, ... lagging strand of ... The lengths of Okazaki fragments are between 1,000 to 2, ... Okazaki ...

Lagging strand

... during DNA replication. The lengths of Okazaki fragments are between 1,000 to 2,000 nucleotides long in ... as the lagging strand . ... reason why Okazaki fragments are created during lagging strand DNA ... newly synthesized DNA where ... As the Okazaki fragments are joined, the new lagging strand becomes ...

Genomic library

... biology, a library is a collection of DNA fragments that is stored and propagated in a population ... collection of cloned deoxyribonucleic acid fragments in a vector; includes all the genetic Library ... biology , a library is a collection of DNA fragments that is stored and ... Applications of genomic ...

Genomic clone

... offers the ability to clone large genomic DNA fragments of between 70-95 ... fractionated genomic DNA ... DNA CLONING (LEC 15) Is the cloning of fragments of CHROMOSOMAL DNA. ... ACs main use are in ... genomic cloning . ... If we want to clone fragments of genomic DNA which are even as small as 20kb ...

Gel electrophoresis

... Agarose Gel Electrophoresis of Restriction fragments ... a simulation of an agarose gel ... a process known as gel electrophoresis , DNA fragments can be separated ... Gel Electrophoresis At a ... Agarose gel electrophoresis separates DNA fragments according to their size. ... restriction ...

Blunt End

... are of the same length. ... Blunt - end DNA fragments can be ligated into Promega's T-Vectors if they ... Blunt - End Pfu DNA Polymerase-Generated PCR fragments into pGEM®-T Vector ... Pfu DNA Polymerase-generated blunt - end PCR fragments can be ligated into the ... Decription ... ...

VNTR

... locus, a number of different alleles may exist, each with ... If the fragments of DNA containing the VNTR alleles are to be separated on the ... ... YELLOWPAGES.COM By using buoyant density gradient centrifugation, DNA fragments with ... One type of minisatellites is called variable number of tandem ...

Overlapping clones

... Service, USDA. Communicating news and ... pattern of the different fragments to identify overlapping BAC clones . ... The researchers tiled ... ... Overlapping Clones : ... by generating randomly overlapping DNA fragments with infrequently ... ...

Oligonucleotide

... Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The ... Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical A community about ...

Northern blot

... procedure that ... Southern blot A technique for separating DNA fragments by electrophoresis and then ... Northern blot analysis (technique ... because it is similar to "Southern" ... In the Northern blotting , RNA fragments are treated with formaldehyde to ensure ... ...

Mitochondrial DNA

... degenerative diseases,1,2,3,4 ... Integration of mitochondrial DNA fragments into nuclear chromosomes (giving rise ... DNA fragments of mitochondrial origin, originating from both coding and ... Argus ...

Macrorestriction map

... ... Genome mapping is the creation of a genetic map assigning DNA fragments to chromosomes. ... Macrorestriction , map ,biologica l,biology ... ... Genome mapping is the creation of a genetic map assigning DNA fragments to chromosomes. ... Macrorestriction is a type of physical mapping ...

Genomic DNA

...organisms,... In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through ... genomic DNA is isolated directly from the white blood cell ... fragments that can be removed and substituted for equally large pieces of genomic ...

Electrophoresis

... ... Agarose gel electrophoresis is an easy way to separate DNA fragments by their ... The technique of electrophoresis is based on the fact that ... usually Tris ... Virtual Lab: Agarose Gel Electrophoresis of Restriction fragments ... High speed electrophoresis in 6 minutes. Length variable ...

DNA ligase

... but is not commonly used. ... T4 DNA ligase will also ligate fragments with blunt ends, ... T4 DNA ligase . ... The optimal incubation ... mean? ... ligase chain reaction. satellite DNA . Z-DNA ... dsDNA fragments in 30 min. T3 DNA ligase was shown to be over 5-fold more efficient ......

Contig

... by its hybridization to one or more cloned deoxyribonucleic acid fragments from an A contig is a set of gel readings that are related to one ... contig map depicts the order of overlapping DNA frag ... between fragments than contig maps (see below) ... Contig construction can be ... DNA ...

Coding strand

... of a new strand in the 5' to 3' direction. ... The lagging strand fragments are called Okazaki fragments after their discoverer, ... What is coding strand ? Meaning of ...

Chromosome walking

... Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain ...

Chromosome walk

... Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain ...

Chromosome jumping

... Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain ...

Agarose gels

... to high- throughput precast agarose gels for resolution of DNA fragments ... E-Gel® EX Precast Agarose Gels ... E-Gel® 48 4% agarose gels ... As depicted also in Fig. 7, agarose ... Agarose gels separate DNA fragments differing by a hundred or more base pairs, ... dishes, know how to ...

Acrylamide gels

... Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in length so the sequence could be read. ...w... The acrylamide gels described in this prep are used to separate fragments of DNA ... The gels will need to sit for ~1.5 hours for the acrylamide ...

Transposase

... the D-D-E motif and thus ... Tn5 transposase is the key enzyme in forming transposomes for random transposon insertions. ... of cut out fragments with transposase molar excess of 0-9x ... ... tnpR, this transposon also carries a transposase or tnpA and a β-lactamase Bla ... This ...

Thallus

... who wrote in Greek. It is uncertain when he wrote, but it was probably in the middle of the first century . His works are all lost, but a few fragments have reached us in later Ancient and Medieval writers. The most interesting of these is ... thallus n. , pl. thalli or -luses . A plant ...

Thrombocytes

... Thrombocytes are cells that play a key role in blood clotting. In mammals, thrombocytes are anucleated cell fragments called platelets. Nucleated thrombocytes of nonmammalian vertebrates differ from the mammalian thrombocytes not only in having a nucleus and ...

Sub-cloning

... in E. coli. 1. Sub - cloning . ... PLoS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC ... Sub - cloning vector-derived fragments requires the donor fragment to be excised ... China to Try Cloning of Rare Monkeys - Using advanced technology to research ... Online - An ...

Southern blotting

... Southern Blot : Part 3. Immunological Detection with the. Genius System. 1. ... Southern blotting is a technique for detecting specific DNA fragments in a complex mixture. ... Southern blotting . ... can be detected by southern blotting . ... Southern Blots analysis of transgenic ...

Shotgun method

... Oct 27 ... ... can only carry small amounts of DNA, then the shotgun method can be used. More commonly, the method is used to generate small fragments of DNA for sequencing. ... Shotguns have long been the preferred method for sport hunting of birds, and the ... shotguns have been getting ...

Shotgun cloning

... processes for duplicating biological material. Discusses ethical, legal, and social issues. Cloning is frequently employed to amplify DNA fragments containing genes, but it ... can be done by using shotgun cloning , c-DNA clones, gene machines (artificial ... The shotgun method (also ...

Sarcoplasmic reticulum

... reticulum exists as particles with minimum ... Sarcoplasmic Reticulum in Duchenne Muscular Dystrophy ... Sarcoplasmic reticulum fragments isolated from dog cardiac muscle possess a ... The sarcoplasmic reticulum preparation produced glucose 1-phosphate and glucose ... ...

RFLP

... ... RFLP analysis play a significant role among others in ... RFLP . Restriction Fragment Length Polymorphism ( RFLP ) analysis. measures fragments ... RFLP testing. requires hundreds of steps and weeks to complete, and it has ... RFLP - Ecology of RFLP from A Dictionary of Ecology at ...

Restriction enzyme

... DNA, thus eliminating infecting organisms. Restriction enzymes can be isolated from bacterial cells and used in the laboratory to manipulate fragments of DNA, such as those that contain genes; for this reason ... Restriction enzymes are one of the most important tools in the recombinant ...

Replication bubble

... wild type (WT) ... post release, suggesting that replication bubbles do not expand in HU ... Replication bubbles were observed in most fragments derived from a 40-kb single ... Replication bubbles , however, were not detected in HH6.3. Bubble arcs also were ... ...

Repetitive DNA

... ... DNA reassociation kinetics is used to classify the repetitive DNA sequences. ... Highly repetitive : About 10-15% of mammalian DNA fragments reassociate very rapidly. ... repetitive DNA - DNA sequences that are present in a genome in ... News archive for repetitive DNA . ...

Recombinant clone

... genes, allowing maximum expression of recombinant antibody/bioproducts. ... FOR RECOMBINANT DNA ISOLATION, CLONING , and ... of PCR fragments for cloning ... Listing of M13 (pUC) cloning sites. Commonly used restriction enzymes ... What is cloning ? Why clone ? Facts and links ...

RAPD

... essays, summaries, research information, and more. ... Application Of Rapd In The Determination Of Genetic ... RAPD markers markers are DNA fragments from PCR amplification of random segments of genomic DNA ... traditional PCR analysis, RAPD (pronounced "rapid") does ... PCR based markers ...

Prometaphase

... Prometaphase is the phase of mitosis following prophase and preceding metaphase, in eukaryotic somatic cells. The nuclear envelope breaks into fragments and disappears. Microtubules emerging from the centrosomes at the poles (ends) of the spindle reach the ... ... stages are prophase, ...

Primary Structure

... ... Primary Structure . • Nucleotide Building Blocks ... 2. The Primary Structure of Peptides ... If the primary structures of these fragments are known, it is sometimes possible ... An Example of Primary Structure Analysis ... Primary structure summary with 9 pages of ...
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