affinity

Vertebrates

... in vertebrates and pH decreases, increasing the affinity of hemocyanin for oxygen ... Full article >>> A group of taxa that includes an ancestral taxon but not all descendants of that taxon - e.g. "fish" is a paraphyletic group because it does not include the other vertebrates , whi...

Transferrin receptor

...HFE ... Full article >>> The strong affinity of the transferrin receptor for trans ferrin (its ligand) ensures that the cell will get all the iron it needs even if trans ferrin represents only a small fr action of the protein molecule s present in the ECF. Full article >>>...

Streptavidin

...;>> Biotin has an extraordinarily high affinity for streptavidin with a reported dissociation constant of ~10-15. This very strong binding affinity has made the biotin - streptavidin system very attractive for a multitude of in vitro labeling app...

Sigma Factor

... proteins in bacteria that increase the binding affinity of RNA polymerase to a promoter . Different si...nscription . These proteins increase the binding affinity of RNA polymerase to a promoter . Different sigma factor s recognize different promoter seque...

Recombinant

...>> Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins that are express ed in Escherichia coli [1] or other prokaryotic expression systems. Full article >>> Bacteria are formally haploid and thus don't go through a mei...

Promoter

...potential,E - a measure of an electron carrier 's affinity for electrons ... Full article >>> promoter A specific nucleotide sequence in DNA that binds RNA polymerase and indicates where to start trans cribing RNA . prophage ... Full article >>> promoter The spec...

Peptides

...nteracting protein pairs, enzymatic digestion , affinity purification, and mass spectrometric ana lysis of the resulting crosslinked peptides to yield information on interacting protein pairs. Full article >>> Many proteins are modified by proteases that remove short peptides from e...

Nucleoli

..... Full article >>> The AB has some affinity to the keratin s. In the mouse cell culture 3T3 line the staining is intranuclei, with fine dots forming chains surrounding dark areas, which do not correspond to the nucleoli . Full article >>>...

Mitotic spindle

...bout 130 bp. It binds several proteins with high affinity to form the kinetochore which is the anchor for the mitotic spindle . Full article >>>...

Mass spectrometry

...f chromatography techniques are used especially affinity chromatography . Full article >>> functions encoded by genes; The ana lysis of all (or most) of the proteins encoded by a genome . Some of the techniques used to character ize the proteins on this scale include bioinformatics...

Lagging strand

...ssion ] lac repressor [Discussion] [isolating by affinity chromatography ] Lactase Lacteal Lactose Lagging strand ( DNA ) Laminins Lamins ... Full article >>> In this case, the DNA end is formed by anneal ing of the 3' end of the leading strand with the 5' end of the lagging strand . ...

Kinetochore

...bout 130 bp. It binds several proteins with high affinity to form the kinetochore ... Full article >>>...

Ion channel

...62;>> Use of a CyP-D fusion protein as affinity matrix has revealed that CyP-D binds very strongly to 1:1 complexes of the voltage-dependent an ion channel (from the outer membrane) and adenine nucleotide trans locase (inner membrane). Full article >>> FM audio for televis ion...

Invertebrates

...n invertebrates and pH decreases, increasing the affinity of hemocyanin for oxygen ... Full article >>> : Having both male and female sexual organ s in one individual. Most invertebrates and plant s are hermaphrodite s. Union of the gametes of the same individual (self- fertilizati...

Immunology

...gies Anatomy of the immune system Antibody affinity Antibody - Antigen Binding Antigen Presentation: how antigens are "presented" to B cells and T cells ... Full article >>> immunology The study of the immune system . This study reveals the many phenomena that are respons...

Hydrolysis

... the ATP results in a recA-ADP complex with less affinity for DNA . The recA-mediated strand exchange proceeds at a few bp/sec. If the moving branch encounters a region where four strand s are present, symmetric strand exchange begins. Full article >>> X-gal is a colorless compound ...

Humoral immunity

..., germinal center formation and isotype switching, affinity maturation and memory cell generation. Full article >>> Humoral immunity is mediated by secreted antibodies , produced in cells of the B lymph ocyte lineage (B cell ). Secreted antibodies bind to antigens on the surfaces...

His tag

...p to: navigation, search A simple column for Ni2+- affinity chromatography . The sample and subsequent buffer s are manually poured into the column. Full article >>>...

Glycoprotein

...ycoprotein which binds to biotin with very high affinity (Kd = 10-15). Full article >>> Variable surface glycoprotein (VSG). One of a battery of antigenic determinant s express ed by a microorganism to elude immune detection. Variation . Differences in the frequency of genes and trait ...

Fusion Protein

...ed clone produced a fusion protein with strong affinity for HS3. Immunofluorescence with antibodies against this fusion protein demonstrated a character istic intranuclear pattern in HeLa cell s and on the isolated nuclear matrix. Full article >>> Tamm is a professor of Biophysics a...

Effector cell

...62;>> Memory TH cells retain the TcR affinity of the origin ally activated T cell , and are used to act as later effector cell s during a second immune response (e.g. if there is re-infection of the host at a later stage). Full article >>> Afferent neuron s convey informat...

Chromatography

...f chromatography techniques are used especially affinity chromatography . Full article >>> ...ds tightly to a specific sugar or sugars. Used in affinity chromatography to purify glycoprotein s and as reagents for detecting glycoprotein s. A B C D E...

Biotin

...;>> Biotin has an extraordinarily high affinity for streptavidin with a reported dissociation constant of ~10-15. This very strong binding affinity has made the biotin - streptavidin system very attractive for a multitude of in vitro labeling app...

Avidin

...ycoprotein which binds to biotin with very high affinity (Kd = 10-15). BACK MUTATION - Reverse the effect...ycoprotein which binds to biotin with very high affinity (Kd = 10-15). Full article >>> Biotin has an extraordinarily high affinity for str...

Attenuation

...ycoprotein which binds to biotin with very high affinity (Kd = 10-15). Full article >>> The pixel itself is displayed according to the mean attenuation of the tissue (s) that it corresponds to on a scale from -1024 to +3071 on the Hounsfield scale . Full article >>> In t...

Alimentary canal

...lectroreceptors) Electron ElectronegativeHaving an affinity for electrons. Full article >>> The intestine is the portion of the alimentary canal extending from the stomach to the anus and, in human s and other mammals , consists of two segment s, the small intestine and the large ...

Affinity chromatography

Affinity Chromatography The goal of affinity chromatography is to separate all the molecule s...ds tightly to a specific sugar or sugars. Used in affinity chromatography to purify glycoprotein s and as reagents for detecting glycoprotein s. A B C D E F...