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Sequencing in Biological Dictionary

Shotgun sequencing

... In genetics, shotgun sequencing , also known as shotgun cloning, is a method used for sequencing long DNA strands. It is named by analogy with ... firing pattern of a shotgun . Shotgun sequencing In genetics , shotgun ...

Shotgun method

... as shotgun cloning, is a method used for sequencing long DNA strands. It is named by analogy with ... that puts antiquity in a new ... Shotgun sequencing In genetics , shotgun sequencing , also known as shotgun cloning , is a ...

Shotgun cloning

... known as shotgun cloning , is a method used for sequencing long DNA strands. It is named by analogy with ... Definition: ... ... cloning followed by DNA sequencing from both ends of the vector is ... This involves shotgun cloning the whole genome and sequencing the clones without ... Back to NRCPB. Back ...

Sequencing

... In genetics and biochemistry, sequencing means to determine the primary structure of an unbranched biopolymer. sequencing results in a symbolic linear depiction known as ... of the sequenced molecule. The term DNA sequencing refers to methods for determining the order of ...

Inverse PCR

... Inverse PCR ... Inverse PCR & Cycle sequencing of P Element Insertions for STS ... Inverse ... Project, USA. ... Inverse PCR & Cycle sequencing of P Element Insertions for STS Generation ... ... Research Center) ... Inverse PCR for PAC-End sequencing (WashU-Zebrafish Genome Resources Project) ... ...

DNA sequencing

... The term DNA sequencing refers to methods for determining the order ... researchers, ... Thus far, most DNA sequencing has been performed using the chain termination ... of nucleotides in ... development of rapid DNA sequencing methods in the early ... DNA sequence The ...

DNA sequence

... The term DNA sequencing refers to methods for determining the order of ... DNA polymerase-catalyzed radiolabeled two-step sequencing ... DNA sequencing : ... polyacrylamide gels for DNA ...

Dideoxy Sequencing

... for the ... Sanger dideoxy terminator sequencing , the ... Dideoxy ... DIdeoxy sequencing Reactions. This is the original M13 sequencing protocol. ... DIDEOXY ...

Chromosome walking

... Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such ... might be mapping. And while you might walk by sequencing minimally the ends of each fragments, I think ...

Chromosome walk

... Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such ... might be mapping. And while you might walk by sequencing minimally the ends of each fragments, I think ...

Transcriptome

... transcriptome data, have already been published: ... Genome & DNA sequencing Services, De Novo Assembly, Mapping of Genomic DNA, PCR, & MicroRNA. ... Maher et al. Transcriptome sequencing to detect gene fusions ... Fully Supported Transcriptome Analysis ...

Recombinant clones

... in ... PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING , and sequencing ... G. Double-stranded sequencing of cDNA clones containing long poly(A) tails using ... Online ...

Primer extension

... recommended for the primer extension reaction depends upon the ... sequencing reactions and primer extension products can be imaged on the LI-COR ... Primer Extension . • Allele Specific Oligonucleotide Ligation. • sequencing ... One can label the primer and apply the extension products to gel ...

Polymorphic loci

... in context ... illustrate how Polymorphic DNA Technologies uses DNA sequencing to score SNPs. The polymorphic locus is highlighted in ... putting ... Sanger dideoxy ... The polymorphic locus can be confirmed by sequencing in ... The overall mean proportion of polymorphic loci was 61.19 ...

Neurospora crassa

... . Ann. Rev. Genet. ... Neurospora crassa RFLP Mapping Program ... sequencing the Genome of Neurospora crassa . Efforts are underway to sequence ... blastn, tblastn or ... Neurospora crassa Evening cDNA Library EST sequencing Data via ftp ... ...

Genomic clone

... of fragments of CHROMOSOMAL DNA. ... ACs main use are in GENOMIC sequencing and in MAP-BASED CLONING of genes ... Genomic , clone ,biological ... is by insertional mutagenesis ... Large Insert Clone / Genomic sequencing Approaches. Viral Vector. Molecular Genetics and Genomics ... All genomic ...

Genome projects

... organization. ... U.S. Department of Energy's center for genome sequencing and science. ... Genome Projects . User Programs. Software Tools. ... its ... The Sanger Institute undertakes a number of large scale sequencing projects that range from complex vertebrates through nematodes and ...

Genome project

... the approximately ... The S288C strain was used in this sequencing project . ... Job Opening with UCSC Genome Browser Project The UCSC ... hypotheses ... U.S. Department of Energy's center for genome sequencing and science. Offers information, data, and results for scientists, and ...

Contig map

... In shotgun DNA sequencing projects, a contig is a set of overlapping DNA segments derived from a ... clones, and ... Rather, development of physical contig maps and sequencing BAC clones work ... Sixty-Eight (68) STS Markers and Corresponding BAC ...

Chromosome jumping

... the same purpose, including chromosome walking. Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long ...

Base sequence analysis

... automated, for determining the base sequence . ... A new method for sequencing DNA. DNA sequence of the ... Adenine :: analysis ; Bacterial Proteins ... the base sequence . ... See base sequence analysis . DNA sequencing ... Base sequence analysis : A method, sometimes automated, for ...

Base sequence

... In genetics and biochemistry, sequencing means to determine the primary structure of an unbranched biopolymer. sequencing results in a symbolic linear depiction known as a sequence which ...

AFLP

... software - the most complete solution for AFLP research. ... AFLP ® DNA sequencing Ecotilling Education Solutions Microsatellites Reverse ... ... AFLP ... an AFLP gel on the ABI is very similar to running an automated sequencing gel. ... ...

Y chromosome

... most other mammals. ... In mammals, the Y chromosome contains the gene that triggers embryonic ... A team of 40 researchers has finished sequencing the Y chromosome , the ... In the case of the human Y chromosome —and likely those of ... Y CHROMOSOME -SPECIFIC MARKERS ... Between X ...

Venter

... ... We have 58 Tandi Venter prints Free Shipping! - Orders $35 or more. www. ... Craig Venter - In 2001, Craig Venter made headlines for sequencing the human genome. ... In 2005, Venter founded Synthetic Genomics, a private company with a ... Venter . Home. About. Contact. RSS. The ...

Synteny

... ... Here's something else researchers can learn from synteny . ... Britannica online encyclopedia article on synteny (genetics), Genomic sequencing and mapping have enabled comparison of the general structures of genomes of many ... Comparative genomics and synteny are hot topics in ...

Sub-cloning

... ... you in setting-up new sub - cloning projects and in preparing the electronic ... Back to NRCPB. Back to Rice genome. SHOTGUN CLONING & sequencing ... At present a lot of effort is going in the sub - cloning activity, as it is very labor intensive. ... ...

Satellite RNA

... protein preferentially ... ... to the satellite RNAs (sat- RNA ) of other necroviruses (39) has been found in ... 3. cDNA synthesis and sequencing of BBSV satellite RNA . ... Barley yellow dwarf virus satellite RNA : Helper - Luteovirus ... It is like a satellite RNA since it ...

Rolling circle

... rolling circle technique: ... This is achieved by a reaction called rolling - circle amplification, or RCA. ... Home > Education > How sequencing is Done > Step 7: Rolling Circle Amplification ... Find Great Bargains for Circle Rolling Bag at NexTag.com. Compare Prices on the ...

RNA editing

... ... The primary benefit of RNA editing could be evolutionary conservation of protein ... First-time application of next-generation sequencing technology to RNA editing analysis ... know of any A-to-I RNA editing lab information not listed here or ... RNA editing by ...

Regulatory region

... glossary with medical definitions ... Definition of Regulatory region . Regulatory region : A region of a chromosome that controls ... sequencing of the regulatory regions for ftz, en and kni was done using ... We also used data for the regulatory regions of eve and hb already available ...

Prokaryote

... articles and research from Academic Journals, Newspapers, and magazines at HighBeam.com. Free Trial, Credit Card Required. Genome & DNA sequencing Services, De Novo Assembly, Mapping of Genomic DNA, PCR, & MicroRNA. ... 454 Home > Applications > Prokaryote Whole Genome. Overview ... ...

Polyacrylamide Gel

... A Polyacrylamide Gel is a separation matrix used in electrophoresis of biomolecules, such as proteins or DNA fragments. Traditional DNA sequencing techniques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in length so ...

Phylogenetics

... In biology, phylogenetics is the study of evolutionary relatedness among various groups of organisms, which is discovered through molecular sequencing data and morphological data matrices. The term phylogenetics is of ... Phylogenetics is the taxonomical classification of organisms ...

Overlapping clones

... for overlapping BAC clones by generating many bands which are specific ... ... overlapping clones (Staden,R. "A strategy of DNA sequencing employing ... Contig: One of a set of overlapping clones that represent a continuous region of ... 1. Finding overlapping clone pairs ...

Nuclein

... mat ... ... in the history of DNA research, beginning from the identification of " nuclein ." Key Words: nuclein • DNA • gene • genome • sequencing • Department of Energy ... Kossel's researches on nuclein , begun ten years after Miescher's discovery, led ... of the chemical makeup of ...

Nuclear genome

... Nature is the international weekly journal of science: a magazine style journal that publishes full-length research ... that nuclear genome sequencing of ... The nuclear genome sequence from this draft assembly was annotated using the JGI ... US Department of Energy Joint Genome ...

Multiplexing

... summary with 6 pages of encyclopedia entries, essays, summaries, research information, and more. ... Multiplexing is an approach to the sequencing ... Find Our Best Deals for Inverse Multiplexing at NexTag.com. Save Big on Accessories for Computers, Electronics, Gadgets and More! Read ...

Molecular phylogeny

... In biology, phylogenetics is the study of evolutionary relatedness among various groups of organisms, which is discovered through molecular sequencing data and morphological data matrices. The term phylogenetics is of ... A site dedicated to Microbiology ... Molecular Phylogeny . ...

Molecular evolution

... the field of molecular evolution came into its own in the 1960s and 1970s, following the rise of molecular biology. The advent of protein sequencing ... We can observe molecular evolution in DNA, and also in amino acid changes in proteins. ... The molecular evolution of an ...
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