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Probe in Biological Dictionary

Single locus probe

... A single locus probe is a DNA or RNA sequence that is able to ... been developed for the first single locus probe , the radioactivity on the ... DNA Forensics ... Set 2. Problem 5: Alleles in a single - locus probe ... individual with a specific single - locus ...

Probe

... Langmuir probe , an electrode inserted into a plasma to determine temperature and ... Periodontal probe , an instrument used in dentistry ... The Huygens probe , supplied by the European Space Agency (ESA) and ...

Oligodeoxyribonucleotide

... arrays, a ... Oligodeoxyribonucleotide probe accessibility on a three-dimensional DNA ... ... laryngeal ... Oligodeoxyribonucleotide probe accessibility on a three-dimensional DNA ... ... conditions, and oligodeoxyribonucleotide probe length can impact ... ...

Northern hybridization

... Sulfate ... 5. Northern analysis using the probe under the same degrees of ... Northern , ... Northern hybridization - hybridization of a probe to filter bound RNA; the RNA ... Under ... greater than 50 bases in ... Using an RNA probe in a Northern instead of a DNA ...

Multi-locus probe

... Multi - locus , probe ,biological ,biology dictionary,biology ... terms,biology abbreviations ... Multi - locus probe . A probe that hybrid izes to a ... ... the multi - ...

Longitudinal axis

... major (or long) axis " as opposed to ... A probe head includes a housing and a probe element. The probe element has a longitudinal axis and stop ...

DNA probes

... In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable ... to the sequence in the probe. The probe thereby hybridizes to single-stranded nucleic ... of 38 culture-positive specimens were PCR/ DNA probe positive for C. jejuni. ... No DNA ...

Stringency

... as the number of ... Especially, the stringency is seldom varied in probe characteristics studies (15, ... This was done for each PM and MM probe pair in the separate stringency zones. ... Strin·gen·cy n. (str ĭ ...

Hybridization

... and data that are helping us to ... MCD Biology 1150 ... Once a probe has been pre-absorbed, you can skip to hybridization . 1. Replace hybridization solution with 1ml probe solution (1µg/ml probe) ... Multicolor fluorescence in situ ...

Terminator region

... • Explain how transcribed RNA is modified ... terminator region of the. DNA. Initiation. • Genes on the DNA begin with a ... A terminator probe vector was constructed by cloning the M. tuberculosis hsp60 ... Promoter Regions (Genetics) Terminator Regions (Genetics) Transcription, ...

Southern blotting

... due ... Southern blotting ... Analysis of Southern blots . Here's a simple example. ... You perform a Southern blot , using the probe sequence marked ABC, and get the ... " Southern blots " is a short-hand term for a rather lengthy procedure that ... In preparation for a ...

Southern blot

... (NWFSC) ... Southern blotting ... Analysis of Southern blots . Here's a simple example. ... You perform a Southern blot , using the probe sequence marked ABC, and get the ... Southern Blot . The Southern blot is used to detect the presence of a particular bit of DNA in a ......

Solution hybridization

... Solution Hybridization Improves Sensitivity ... Ambion makes this solution hybridization assay available as the mirVana miRNA ... The probe concentration in the hybridization solution should be 10 ng/mL if the ... Pour off the hybridization solution into the radioactive waste, ...

Simple Sequence Repeat

... simple sequence repeat ) is a general term for a genome region ... Simple Sequence Repeat DNA Length Polymorphisms. Published in probe Volume 2(1): Spring 1992 ... termed a Simple Sequence Repeat (SSR) (Jacob et al., 1991) ... Microsatellites, or Simple Sequence Repeats ...

RT-PCR

... by Dr Jack Vanden Heuvel, ... I: BASIC RT - PCR RECIPES. APPENDIX ... ... Transcription, Polymerase Chain Reaction ( RT - PCR ) Primer and probe Set for ... with the FDA-cleared laboratory RT - PCR assay should be conducted in conjunction ... Reverse Transcriptase- PCR ... The following ...

Ribosomal DNA genes

... are located in a ... ribosomal DNA genes in the. ribosomal DNA genes in the ... Fingerprinting of Ribosomal DNA . Genes (OFRG) probe selection. Clustering binary vectors with ... News section. News release. 15,713. Back to main news page ... ... and in the small- and ...

RFLP

... where restriction enzymes can RFLP . Restriction fragment length polymorphisms (RFLPs) were the first type of ... blotting with a probe specific for a region of DNA known to contain an RFLP . ... The three most common types of markers used today are RFLP , RAPD and isozymes. ... ...

Primer extension

... solution based, sequence specific ... Design oligos for allele specific primer extension assays based on Luminex xMAP technology, capture probe design, multiplex assay design, bio-rad bioplex Primer Extension . • Allele Specific Oligonucleotide Ligation. • Sequencing ... One can label ...

Positional cloning

... reverse genetics, the term for identifying mutant alleles in genes ... Functional cloning involves using a known gene sequence as a probe to look for new gene sequences that may have similar functions, based on the similarity of sequence. Using this method of analysis, researchers ...

Polysome

... and glycerol gradients. ... Cell lines were exposed to 2 Gy, polysome -bound RNA isolated 6 hours later, and ... Polysome preparation and probe labeling were performed as described (3) ... The highest amount of polysome -bound PMR1 was observed prior to estrogen ... Preparation of ...

Peptidyl transferase

... it is ... Binding of CCdApPuro by a peptidyl transferase -like motif in the ... peptidyl transferase center that may serve as a probe and/or ... The peptidyl transfer is a nucleophilic attack of the α-amino group of the. aa-tRNA on the ester carbonyl group of peptidyl -tRNA. ...

Oligonucleotide

... useful in current laboratory practice because ... oligonucleotide n. A short polymer of two to twenty nucleotides. ... What oligonucleotide probe would best hybridize with the DNA sequence 5'-AAAAGGTTCC-3' ... Oligonucleotide synthesis Oligonucleotide synthesis is the chemical ...

Northern blot

... Northern blot is used to analyze RNA. ... Northern blots are intended to study gene expression through ... ized, do NOT proceed with probe purification or Northern blot hybridization. ... Pre-hybridize Northern blot for a minimum of 1 hour at 42°C in Ambion ULTRAhyb™–Oligo ... ...

Nick translation

... is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in Fluorescent in situ hybridization or blotting techniques. Nick translation summary with 2 pages of encyclopedia entries, essays, ...

Minicircles

... Minicircles , however, are unconventional, ... Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles ... to probe for the existence of kinks in 94-bp-long minicircles . ... Preparation of mitochondrial telomeric minicircles by alkaline lysis ... To relax ...

Macrorestriction map

...... Mapped Drive - Map reproduction - MAP /TOP - MAPD - MAPI method - Mapimi Basin ... A macro-restriction map was constructed by linking probe hybridisation of SwaI ... positions on the SwaI macro-restriction map according to the individual sequence ... Through hybridization of ...

In situ hybridization

... situ hybridization , it is possible to localize gene expression to ... The first step of in situ hybridization is selection of a probe type. ... Solutions for in situ hybridization ... Preparation of In Situ Hybridization buffer : ... After in situ ...

Homeothermic

... homeothermic in the Columbia Encyclopedia, Computer Desktop Encyclopedia, computing dictionary. Homeothermic heating systems have a thermal probe to monitor the animals temperature, ... rats and larger animals, for use with Homeothermic System ... Words similar to ...

Genome projects

... to determine the complete DNA sequence of the euchromatic genome of the fruitfly, and to develop experimental and computational tools to probe its ... The Sanger Institute undertakes a number of large scale sequencing projects that range from complex vertebrates through nematodes ...

Genome project

... to determine the complete DNA sequence of the euchromatic genome of the fruitfly, and to develop experimental and computational tools to probe its ... ...

Genetic distance

... Distance Ratio for chromosome I is shown below. ... Genetic distance and trade flows are strongly correlated. ... classification) to probe further the relationships between genetic distance , geography, and ... Generally speaking, a genetic distance of 0, 1, or 2 on a ...

Drosophila

... to determine the complete DNA sequence of the euchromatic genome of the fruitfly, and to develop experimental and computational tools to probe its ... Note: click on a word meaning below to see its connections and related words. The noun Drosophila melanogaster has one meaning: Meaning ...

DGGE

... samples) by DGGE (data not shown) ... screened by PCR- DGGE by using the ... Denaturing gradient gel electrophoresis ( DGGE ) was used to probe for mutations ... Five DGGE primer sets that scanned for DNA alterations across 775 bp of rpoB ... ...

Denature

... electrophoresis ... NOTE: To denature Labeled DNA for a hybridization application, please see ... Do not heat- denature the Labeled DNA probe before this step. ... ... as boiling of clinical material to denature proteins and disrupt virions.1, 2 ... to denature proteins and ...

CDNA array

... array is an independent assay for the presence of a different cDNA . ... Once the cDNA probes have been hybridized to the array and any loose probe has ... We have constructed several versions of our potato cDNA array . ... The clone list for the potato 10K version 4 cDNA array is ...

Band shift assay

... retardation assay . EMSA is used to study protein ... DNA Band Shift Assay . DNA Band Shift Assay ... A. Making the Band Shift probe ... 1. Mobility shift DNA-binding assay using gel electrophoresis. ... Thus, the gel- shift assay indicates the ability of a protein(s) ...

Arrayed library

... HT Array Plate library files are intended for use with GeneChip ... Support, Library File Updates, NCBI Version 35, BPMAP, BAR, BED ... probe BED files - separate file for each array in the set. ... 10 ArrayRef library . 11 Unsafe operations and running over array elements. 12 ...

Arabidopsis

... Genome Initiative (AGI) has recently been launched to complete ... The Arabidopsis 1.0R Array is a single array comprised of over 3.2 million probe pairs tiled through the complete non-repetitive Arabidopsis thaliana genome. ...

Allele-specific PCR

... Allele Specific Oligonucleotide (or ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, ... In Allele - specific PCR the opposite is done. ... In vivo ...
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