Phosphodiester Bond

... A phosphodiester bond is a group of strong covalent bonds between ... and two other molecules over two ester bonds. phosphodiester bonds are central to all life on Earth, as they ... backbone of the strands of DNA. In DNA ... phosphodiester bond This article does not cite any references ...

Ligase

... DNA Ligase catalyzes the formation of a phosphodiester bond between ... Is Taq DNA Ligase used for a ... technique? ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate ... What ... The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between ... The T4 DNA Ligase binding to ...

DNA ligase

... ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' ... end and ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and ... T4 DNA Ligase catalyzes the formation of a phosphodiester ... T4 DNA Ligase is strongly inhibited by ...

Deoxyribonuclease

... is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Deoxyribonucleases are thus one type of ... gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate ...

S1 nuclease

... it can also occasionally introduce single-stranded breaks in ... A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids. Older publications may use terms such as "polynucleotidase" or ...

Ribozyme

... A ribozyme is an RNA molecule that catalyzes a chemical reaction. Many natural ribozymes catalyze either the hydrolysis of one of their own phosphodiester bonds, or the hydrolysis of bonds in other RNAs, but they have ... 6 The L1 Ligase Ribozyme Crystal Structure. 6.1 A Three-Dimensional ...

Ribonuclease

... code 2BNH) ... authors methodology in ribonuclease extraction? What is a ... Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond ... Ribonuclease A has a molecular weight of 13,700 daltons. ... Ahmad, F.: Free Energy Changes in Denaturation of Ribonuclease A by ...

Restriction

... on a ... Restriction enzymes are enzymes that cut DNA at specific recognition sequences ... Restriction enzymes hydrolyze the phosphodiester backbone once on each strand ... Taq I, by the way, is a restriction enzyme from the same type of organism that ... ...

Nuclease

... A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids. Older publications may use terms such as "polynucleotidase" or ...

Exonuclease

... are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close ... exonuclease activity. • protein binding. • hydrolase activity ... Human ...

Endonuclease

... Endonucleases are enzymes that cleave the phosphodiester bond within a ... Restriction endonucleases (Restriction Enzymes) cleave DNA at specific sites, ... Specific cleavage of simian virus 40 ...