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Affinity in Biological Dictionary

Streptavidin

... biology through its extraordinarily strong affinity for biotin ; the dissociation constant (Kd) of ... found in Streptomyces griseus which has a high affinity for biotin and is used ... structure of a ... Home > Ig/2nd Step > Streptavidin ... affinity purified streptavidin can be supplied in batch ...

His tag

... ( His tag ) ... ... Tag Purification. affinity Tag Purificaton Columns. His Tag ... His - ... Purification ColumnSpin FormatHIS-Buster Cobalt affinity Gel is a ... ... Kits " His Tag ... " Protein Biochemistry " Protein Purification " affinity Tag ... his - tag . Dictionary terms for ...

Affinity chromatography

... affinity chromatography is a method of separating ... enzyme and substrate, or receptor and ligand. affinity ... Chromatography is the collective term ... phase, which separates the analyte ... affinity chromatography is a method that overcomes this ...

Fusion Protein

... with functional properties derived from each of ... The Tandem affinity Purification (TAP) is a technique for studying protein - protein ... (Fluc) fusion protein was transiently transfected into ... affinity purification of GST fusion proteins ... PopCulture extraction and ...

Epitope

... ... Deciphering epitope specificities within polyserum using affinity selection of ... Generation, Characterization and Epitope Mapping of ... ... Specializing in the production of polyclonal antibodies, antisera, affinity purified antibodies, fluorescein and ... Epitope Tags. Secondary ...

Band shift assay

... assay , band shift assay , or gel retardation assay , is a common affinity electrophoresis technique used ... ETH Zuerich ... by band - shift ... ... The procedure described in this chapter for the determination of affinity constants and kinetic dissociation constants by band - shift assay ...

Avidin

... lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is not necessary for this activity. ... of ... Streptavidin is similar in properties to avidin but has a lower affinity for ... Online Shopping at DirectTextBook.com - Save up to 80%, buy or ...

Western blotting

... of proteins from sodium dodecyl ... A Western blot is a method in molecular biology to detect a certain protein in a ... Some high affinity monoclonal antibodies can also be used for Western blots . ... Northern and then Western blots were named by analogy. Blotting ...

Vitelline membrane

... was found in Taber's Medical Dictionary Online, along with 60,000 other medical and nursing definitions and dictionary terms. ... A lectin with an affinity for certain sulphated polysaccharides, such as fucoidin and dextran sulphate, has been isolated from the vitelline membrane of hens' eggs ... ...

Somatic hypermutation

... Somatic hypermutation is a regulated mutational process that introduces base ... As a result of somatic hypermutation and selection, Ab affinity for Ag increases ... Although some properties of somatic hypermutation (SH)3 have been well ... Somatic hypermutation of immunoglobulin ...

Phase variation

... 40 percent of ... Michael Hermanussen (2007) " Phase Variation in Child and Adolescent Growth, ... Phase variation can also influence the affinity of pathogens for different host ... The genetic mechanism of phase variation in many cases is expansion and ... Consortium Publishes Phase ...

Peptide-binding groove

... the complex, as well as to a method of ... ... classes is the open-ended peptide - binding groove of MHC class II molecules ... measurable affinity of peptide for the MHC binding groove , in particular the ... peptide - binding groove . The crystal structure of the ... ensconced in the ...

N-terminus

... ... of the conceptual protein and the observed N-terminus is shown in parenthesis. ... IMPACT (Intein Mediated Purification with an affinity Chitin-binding Tag) utilizes the inducible self-cleavage ... C-terminus or N-terminus of the target ... This suggests that the H2B N-terminus ...

Intein

... England Biolabs, Inc. Includes links to publications at the NCBI, National Library of Medicine, ... IMPACT ( Intein Mediated Purification with an affinity Chitin-binding Tag) ... pTXB1 and pTXB3 contain a mini- intein with alternative preferred residues at the ... Intein An intein is a segment ...

Hemoglobin

... - updated ... In adults, fetal hemoglobin production can be reactivated pharmacologically, ... blood cells, this substance decreases the affinity of hemoglobin for oxygen. ... The myoglobin and hemoglobin page provides a description of the structure and function of these two ... ...

Gel shift assay

... electrophoresis, also referred as a gel shift assay , gel mobility shift assay , band shift assay , or gel retardation assay , is a common affinity electrophoresis technique used to study protein-DNA ... The gel . shift assay is performed by incubating a purified protein, or a complex ...

Family

... Family , denotes a group of people or animals affiliated by a consanguinity, affinity or co-residence. Although the concept of consanguinity originally referred to relations by "blood," anthropologists have argued that .....

Exonuclease

... is the molecular weight of Exonuclease T? ... A highly processive enzyme that acts in the 5' to 3' direction, ... Exonuclease Reaction ... affinity chromatography, Lambda Exonuclease is cleaved from the ... Definition of exonuclease from the Merriam-Webster Online Dictionary with audio ...

Cooperativity

... among the ... Usually, cooperativity is understood operationally ... Cooperativity in Molecular Recognition ... cooperativity to provide the affinity and ... Cooperativity has not previously been applied to chemical sensing. ... was the first to deduce the essential feature of cooperativity : ....

Consanguinity

... of being descended from the same ancestor as ... consanguinity n. , pl. -ties . Relationship by blood or by a common ancestor. A close affinity or ... Today consanguinity is a genetic concept that influences ... The term here means, within certain limitations defined by the law of ...

Competitive inhibition

... inhibition is a type of enzyme inhibition that reduces the maximum rate of a chemical reaction (Vmax) without changing the apparent binding affinity of the catalyst for the substrate . What is competitive inhibition ? Meaning of competitive inhibition ... 19) Competitive inhibition ...

C Terminus

... in Wingless signaling. ... Finally, we found that the C terminus plays a role in Armadillo protein stability in ... addition of a biotin affinity tag at the extreme C terminus . We have ... PDE C terminus interacts primarily with the 3 helix and conserved. switch regions (1, 19) ... ...

Binding site

... for you to turn on ... Gel filtration data also suggested the presence of a third binding site of 5.4 kDa. The 170-kDa site had a binding affinity of Kd = 12 x 10-6 and bound both LH ... E. coli DNA- Binding Site Matrices Applied to the Complete E. coli K12 Genome. Summary ... Known ...

Alternative splicing

... ... Using a novel in vivo alternative splicing assay, we show that intron length has ... Alternative splicing may also regulate the affinity of CD44 for hyaluronic acid, ... Alternative splicing --the post-transcriptional editing process that can result ... Research shows that ...
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