Electrophysiology
... recordings:
1. Intracellular recording. This
technique entails impaling a cell, usually a neuron , with ... voltage (current-clamp) across the membrane. This
technique is widely used when recording from brain slices ... animals.
2. Extracellular recording. In this
technique an electrode is placed on the extracellular ...
Genetic fingerprinting
... , DNA testing and DNA profiling is a
technique to distinguish between individuals of the same ... . Next, the bands of DNA are transferred via a
technique called Southern blotting from the agarose gel ... DNA fingerprinting.
Recently, an additional
technique for genetic fingerprinting has been introduced: ...
Molecular biology
... molecular biology as:
... not so much a
technique as an approach, an approach from the viewpoint of ... using viruses as a carrier. In such cases, the
technique is called viral transduction, and the cells are ... chain reaction is an extremely versatile
technique for copying DNA. In brief, PCR allows a single ...
Magnetic resonance imaging
...
1 Background
1.1 Nomenclature
1.2
technique
2 Application
2.1 Safety
2.2 ... devices operating on the same principles.
technique
Medical MRI most frequently relies on ... contrast agent (such as gadolinium) or using a
technique known as "flow-related enhancement" (e.g. 2D and ...
Polymerase chain reaction
... Chain Reaction (PCR) is a molecular biological
technique for amplifying (creating multiple copies of) ... of DNA.
Patent wars
The PCR
technique was patented by Cetus Corporation , where Mullis ...
Genetic fingerprinting is a forensic
technique used to identify a person by comparing his or her ...
Proprioception
... through study of many disciplines. The Alexander
technique and related methods use the study of mannerisms ... can move to another concern. Alexander
technique addresses these issues.
People who have a limb ... of Dancing
A comprehensive Alexander
technique site
...
Reverse genetics
... made disrupting gene expression an accessable
technique for many more investigators. So phenotype, rather ... in the yeast genome.
Another reverse genetics
technique is the application of RNA interference . RNAi ... to humans.
Finally, a more difficult genetics
technique is the creation of transgenic organisms that ...
Aseptic technique in microbiology
... Microbiologists and students of microbiology may use an aseptic
technique to attempt to keep specimens of microorganisms free of microbial contamination. People may commonly use the aseptic
technique when transferring bacteria from one test tube or flask to another. ...
Exon
... to as non-coding RNA .
Exon trapping is a molecular biology
technique that exploits the existence of the intron-exon architecture to find new ... pre-mRNA using Morpholino antisense oligos. This has become a standard
technique in developmental biology .
See also
Peptide
External ...
Gene therapy
... descendants for the life of the transplanted individual.
This
technique is best used for diseases where the desired cells can be extracted easily, ... patient. The missing gene is now replaced and the disease is cured. This
technique is called gene therapy .
Three types of viruses are currently used as ...
Restriction fragment length polymorphism
... sequences) by which they may be distinguished, and as the laboratory
technique which uses this characteristic to compare DNA molecules. The
technique is utilized in genetic fingerprinting and paternity testing .
...
Stem cell
... led by James Thomson at the University of Wisconsin first developed a
technique to isolate and grow the cells. Embryonic stem cell researchers are ... . The Paris University team, lead by Professor Luc Douay, devised a
technique which involves three steps: 1. Take haematopoietic stem cells, which are ...
Y chromosome
... by following the template on the homologous chromosome . This repair
technique is called recombination, and repairs a great many errors. Errors not caught by this
technique are weeded out over time through natural selection. Until recently such ...
Andrew Huxley
... decades later.
The experimental measurements on which the pair based their action potential theory represent one of the earliest applications of a
technique of electrophysiology known as the "voltage clamp". The second critical element of their research was the so-called giant axon of Atlantic squid ( ...
Antibody
... applications
In biochemistry , antibodies are used for immunological identification of proteins, using the Western blot method. A similar
technique is used in ELISPOT and ELISA assays, in which detection antibodies are used to detect cell secretions such as cytokines or antibodies. Antibodies ...
Bioinformatics
... homology is used to determine which parts of the protein are important in structure formation and interaction with other proteins. In a
technique called homology modelling, this information is used to predict the structure of a protein once the structure of a homologous protein is known. This ...
Brain
... allows scientists to record the electrical activity of individual neurons or fields of neurons, but since it requires invasive surgery, this is a
technique usually reserved for lab animals. By placing electrodes on the scalp, electroencephalography (EEG) measures brainwaves, which are the mass changes ...
Chromatography
... Links
History
It was the Russian botanist Mikhail Tsvet (Mikhail Semyonovich Tsvet) who invented the first chromatography
technique in 1901 during his research on chlorophyll . He used liquid-adsorption columns to separate plant pigments . The method was described on December ...
Chromosome walking
... does not allow long DNA strands to be sequenced, this method works by dividing the long sequence into several consecutive short ones.
The basic
technique is as follows:
A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand complementary to the ...
Bioinformatics
... homology is used to determine which parts of the protein are important in structure formation and interaction with other proteins. In a
technique called homology modelling, this information is used to predict the structure of a protein once the structure of a homologous protein is known. This ...
Gel electrophoresis
... such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative
technique to partially purify molecules to use other methods such as mass spectrometry , PCR , cloning , DNA sequencing , or immuno-blotting for further ...
Fermentation
... term is also sometimes used to refer to any chemical mixing process.
Brewing has a very long history, and archeological evidence tells us that this
technique was used in ancient Egypt . Descriptions of various beer recipes can be found in Sumerian writings, some of the oldest known writing of any ....
Francis Galton
... proposed by Dr Henry Faulds in 1880. In a Royal Institution paper in 1888 and three books ( 1892 , 1893 and 1895 ) Galton wrote about the
technique (inadvertently sparking a controversy between Herschel and Faulds that was to last until 1917 ), identifying common pattern in fingerprints and ...
Gel electrophoresis
... such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative
technique to partially purify molecules to use other methods such as mass spectrometry , PCR , cloning , DNA sequencing , or immuno-blotting for further ...
Human Genome Project
... by a company called Celera Genomics (founded by Craig Venter ) while the HGP was still being pursued. Celera Genomics used a newer, albeit riskier
technique called shotgun sequencing and proceeded at a faster pace and at a fraction of the cost of the taxpayer-funded project (approximately $3 billion ...
Insulin
... use, much reducing impurity reaction problems. Eli Lilly marketed the first such synthetic insulin, Humulin, in 1982 . Genentech Inc developed the
technique Lilly used.
Modes of administration
Unlike many medicines, insulin cannot be taken orally. It is treated in the gastrointestinal tract ...
Ion channel
... Nobel Prize -winning theory of the nerve impulse, published in 1952. Channel's existence was confirmed in the 1970s with an electrical recording
technique known as the "patch clamp," which led to a Nobel Prize to Erwin Neher and Bert Sakmann, the technique's inventors. Hundreds if not thousands of ...
Kary Mullis
... Kary Banks Mullis (born December 28 , 1944 ) is a biochemist . In the 1980s , he invented the polymerase chain reaction (PCR), a central
technique in molecular biology which allows the amplification of specified DNA sequences.
He was awarded the Nobel Prize in Chemistry and the Japan ...
Karyotype
... different probes specific of one chromosome pair, carrying different amounts of a set of fluorescent dyes, are hybridized to the chromosomes in a
technique known as fluorescent in situ hybridization (FISH). This gives each chromosome pair unique spectral characteristics due to the relative amount of ...
Liver
... allografts for transplant usually come from non-living donors who have died from fatal brain injury. Living donor liver transplantation is a
technique in which a portion of a living person's liver is removed and used to replace the entire liver of the recipient. This was first performed in 1989 for ...
Malaria
... and southern Europe , but the draining of wetland breeding grounds and better sanitation eliminated it from affluent regions.
Sterile insect
technique is emerging as a potential method to control malaria-carrying mosquitos.
Progress towards transgenic, or genetically modified insects suggests ...
Molecular evolution
... technique, which researchers use to date when two species diverged by comparing their DNA, deduces elapsed time from the number of differences. The
technique was inspired by the once common assumption that the DNA error rate is constant--not just over time, but across all species and every part of a ...
Molecular genetics
... links
Forward genetics
One of the first tools available to molecular geneticists is the forward genetic screen . The aim of this
technique is to identify mutations that produce a certain phenotype . A mutagen is very often used to accelerate this process. Once mutants have been ...
Osmosis
... high solute concentration to a region of low solute concentration by applying a pressure in excess of the osmotic pressure. This reverse osmosis
technique is commonly applied to purify water. Sometimes the term " forward osmosis " is used for osmosis, particularly when used for rehydrating dried food ...
Paleontology
... and increasingly sets these species in a genealogical framework, showing their degrees of interrelatedness using the still somewhat controversial
technique of " cladistics ".
The primary economic importance of paleontology lies in the use of fossils to determine the age and nature of the rocks that ...
Parasitology
... about their relationship with the host (eg. influences on mortality rate) and relationships between different populations of host species. This
technique is commonly applied in fisheries biology , where parasites are used to identify different populations of the same fish. This forms an interesting ...
Sequencing
... of a DNA or RNA strand. Currently, all sequencing is performed using the chain termination method , created by Frederick Sanger . This
technique can only be used to identify fairly short sequences at a time (around 300-1000 base pairs on ABI machine), and therefore strategies to have been ...
X-ray crystallography
... X-ray crystallography is a
technique in crystallography in which the pattern produced by the diffraction of x-rays through the closely spaced lattice of atoms in a crystal is ...