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tag in Biological Definition

Bioinformatics

... with multiple techniques including microarrays , expressed cDNA sequence tag ( EST ) sequencing, serial analysis of gene expression (SAGE) tag sequencing, massively parallel signature sequencing (MPSS), or by ...

Bioinformatics

... with multiple techniques including microarrays , expressed cDNA sequence tag ( EST ) sequencing, serial analysis of gene expression (SAGE) tag sequencing, massively parallel signature sequencing (MPSS), or by ...

Affinity chromatography

... an organism is immunised against a GST-fusion protein it will produce antibodies against the fusion-protein, and possibly antibodies against the GST tag as well. The protein can then be covalently coupled to a solid medium such as sepharose or agarose (these are commercially available). For ...

Endoplasmic reticulum

... proteins are moved across the ER membrane. Proteins that are transported by the ER and from there throughout the cell are marked with an address tag that are called a signal sequence . Gnter Blobel was awarded the 1999 Nobel Prize in Physiology or Medicine for his discovery of these signal ...

Expressed sequence tag

... An expressed sequence tag or EST is a short sub-sequence of a transcribed protein -coding or non-protein coding DNA sequence . It was originally intended as a way to ...

His-tag

... for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole . Detection The his tag can also be used in detection of the protein via anti-his-tag antibodies . This can be useful in localization , ELISA , western blots or other ...

Polymerase chain reaction

... also be a way of mass-producing useful proteins--for example, medicines or enzymes in biological washing powders. The incorporation of an affinity tag on a recombinant protein will generate a fusion protein which can be more easily purified by affinity chromatography . Figure 5 : ...

Endoplasmic reticulum

... proteins are moved across the ER membrane. Proteins that are transported by the ER and from there throughout the cell are marked with an address tag that are called a signal sequence . Gnter Blobel was awarded the 1999 Nobel Prize in Physiology or Medicine for his discovery of these signal ...

Endoplasmic reticulum

... proteins are moved across the ER membrane. Proteins that are transported by the ER and from there throughout the cell are marked with an address tag that are called a signal sequence . Gnter Blobel was awarded the 1999 Nobel Prize in Physiology or Medicine for his discovery of these signal ...

Endoplasmic reticulum

... proteins are moved across the ER membrane. Proteins that are transported by the ER and from there throughout the cell are marked with an address tag that are called a signal sequence . Gnter Blobel was awarded the 1999 Nobel Prize in Physiology or Medicine for his discovery of these signal ...
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