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Sequencing in Biological Definition

Bioinformatics

... DNA sequences from fragmentary "shotgun" DNA sequencing , and the prediction of gene regulation with ... amounts of DNA data (pictured) produced by gene sequencing projects is just one of the tasks faced by ... of this sequence alignment is used in the sequencing process itself. The so-called ...

Chromosome walking

... is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a ... which becomes more popular for large-scale sequencing (e.g., the Human Genome Project ) is shotgun sequencing . See also ...

Bioinformatics

... DNA sequences from fragmentary "shotgun" DNA sequencing , and the prediction of gene regulation with ... amounts of DNA data (pictured) produced by gene sequencing projects is just one of the tasks faced by ... of this sequence alignment is used in the sequencing process itself. The so-called ...

Human Genome Project

... a newer, albeit riskier technique called shotgun sequencing and proceeded at a faster pace and at a fraction ... genome projects in biology, each aimed at sequencing the DNA of a specific organism. While the human ... and medicine are predicted as a result of the sequencing of model organisms including mice, fruitflies, ...

Primer

... that involve DNA polymerases, such as DNA sequencing and polymerase chain reaction (PCR), require ... but order them by specialized companies. DNA sequencing is used to determine the nucleotides in a DNA strand. A sequencing method called ...

Sequencing

... article. In genetics and biochemistry , sequencing means to determine the primary structure (or primary sequence) of an unbranched biopolymer . sequencing results in a symbolic linear depiction known as a ... sequenced molecule. In genetics terminology, sequencing is determining the nucleotides of a DNA or ...

Gel electrophoresis

... to use other methods such as mass spectrometry , PCR , cloning , DNA sequencing , or immuno-blotting for further characterization. The first part, " ... " Chain termination method " page for an example of a polyacrylamide DNA sequencing gel. Gel electrophoresis of proteins is usually done in an SDS ...

Gel electrophoresis

... to use other methods such as mass spectrometry , PCR , cloning , DNA sequencing , or immuno-blotting for further characterization. The first part, " ... " Chain termination method " page for an example of a polyacrylamide DNA sequencing gel. Gel electrophoresis of proteins is usually done in an SDS ...

Molecular evolution

... has been made possible by the availability of techniques for gene sequencing , which allow the determination of the exact sequence of nucleotides or ... chromosome . Typical molecular systematic analyses require the sequencing of around 1000 base pairs . The neutral theory Main article: ...

Primary structure

... to cover the sequence space (non-redundant sequences ). See also sequencing DNA sequence protein sequencing secondary structure tertiary structure quaternary structure ...

Proteomics

... Protein identification . Well-known methods include low-throughput sequencing through Edman degradation. Higher-throughput proteomic techniques are ... commonly peptide mass fingerprinting on simpler instruments, or de novo sequencing on instruments capable of more than one round of mass spectrometry. ...

Reverse genetics

... an investigation. With the readily performed modern techniques of DNA sequencing and as a result of the sequencing of many whole genomes , many genetic sequences are discovered in advance ...

Y chromosome

... in Cambridge, Mass.; Richard K. Wilson, Ph.D., director of the Genome sequencing Center at Washington University School of Medicine in St. Louis; and Robert H. Waterston, M.D., Ph.D., formerly of Washington University's sequencing center and now at the University of Washington, Seattle, discovered that ...

Biopolymer

... using gel electrophoresis and capillary electrophoresis. See also: condensation polymers , DNA sequence , biomaterials , Melanin , sequencing ...

Cladistics

... trees. However, by and large the phylogenetic approach to systematics has proven useful and coherent and has gained general support. As DNA sequencing has become easier, phylogenies are increasingly often constructed with the aid of molecular data. Computational systematics allows the use of ...

Endosymbiont

... for the bacteria is that it is protected from the environment outside the insect cell, and presumably receives nutrients from the insect. Genome sequencing reveals that obligate bacterial endosymbionts of insects have among the smallest of known bacterial genomes and have lost many genes that are ...

Endosymbiont

... for the bacteria is that it is protected from the environment outside the insect cell, and presumably receives nutrients from the insect. Genome sequencing reveals that obligate bacterial endosymbionts of insects have among the smallest of known bacterial genomes and have lost many genes that are ...

Expressed sequence tag

... a way to identify gene transcripts , but has since been instrumental in gene discovery and sequence determination. An EST is produced by one-shot sequencing of a cloned mRNA , and the resulting sequence is a relatively low quality fragment whose length is limited by current technology to approximately ...

Glycomics

... have 20, the saccharides have a multitude of building blocks. Advances in glycomics are anticipated to be driven by improvements in molecular sequencing and bioinformatics , which is the computational organization and processing of sequence data. External links An article on glycomics ...

Isozyme

... Molecular markers for this purpose. Although they have now been largely superseded by more informative DNA -based approaches (such as direct DNA sequencing , Single nucleotide polymorphisms and microsatellites ), they are still amongst the quickest and cheapest marker systems to develop, and remain ...

Phenotype

... enable prediction of the phenotype. Nevertheless, because phenotypes are much easier to observe than genotypes (it doesn't take chemistry or sequencing to determine a person's eye color), classical genetics uses phenotypes to deduce the functions of genes. Breeding experiments can then check these ...

Taxonomy

... recent years taxonomic classification has gained support from molecular systematics , a branch of bioinformatics that employs the method of gene sequencing to construct phylogenetic trees . See also systematics scientific classification ontology cladistics Integrated Taxonomic ...
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