Affinity chromatography

... showTocToggle("show","hide") 1 General procedure 2 Specific Uses 3 Batch verses column ... Chromatography Protocols General procedure Usually the starting point is an undefined ... glutathione is used. Another use for the procedure is the affinity purification of antibodies from ...

Polymerase chain reaction

... 3 PCR in practice 3.1 Primers 3.2 procedure 3.3 Example 3.4 PCR optimization 3.5 ... help design primers (see External links ). procedure The PCR process consists of a series of twenty ... . Agarose gel electrophoresis is a procedure that consists of injecting DNA into agarose gel ...

Cloning

... as an entirely different kind of operation. Therapeutic cloning is the procedure for creating stem cells genetically compatible with the patient. ... first pet cloned by the company after the death of the original cat. The procedure is still very expensive and has little demand. However, demand could be ...

Limbic system

... emotional disorders, this connection was sometimes surgically severed, a procedure of psychosurgery . Patients who underwent this procedure often became passive and lacked all motivation. ...

Magnetic resonance imaging

... images produced by an MRI scanner are used to guide a minimally invasive procedure intraoperatively and/or interactively. However, the non-magnetic ... the use of the data for generating pictures showing different tissues. The procedure as described would take a very long time to perform. There is a big ...

Stem cell

... have been taken from the blood instead of the bone-marrow, making the procedure safer for older people. Although normally scarce, the number of ... the patient. The heart is then restarted and incisions closed. The procedure is minimally invasive, as far as heart surgeries are concerned. All of ...

Ames test

... causes a change in the DNA (deoxyriboneucleic acid) of a living cell or organism. The test is named after its inventor, Bruce Ames . General procedure This assay is carried out using strains of bacteria , generally Escherichia coli or Salmonella that already have a single mutation, for ...

Aseptic technique in microbiology

... , through the top of the blue part of the flame. The person would not allow the loop to touch anything except the specimen itself, until the entire procedure is finished. Preparing to execute the specimen transfer, the person would hold both of the tubes or flasks in one hand , probably the opposite of ...

Dialysis

... , heating pads and solar radiation (often using the dashboard of a car, for instance while travelling). The patient, who performs the entire procedure themselves, dons a disposable surgical mask , scrubs their hands using antibacterial soap, and tucks a clean towel into the waistband of their ...

Ploidy

... = simplification) is the process of creating a haploid cell from a diploid cell. This is a laboratory procedure that forces a normal cell to spit out half of its chromosome content, leaving just one set. In mammals this renders this cell equal to sperm or ...

Enzyme

... uric acid Purification Since enzymes are proteins , enzyme purification begins with protein purification . Each step in the purification procedure is monitored for enzyme activity. One caveat is that proteases (enzymes that digest proteins) can be expected to start digesting themselves as they ...

Ploidy

... = simplification) is the process of creating a haploid cell from a diploid cell. This is a laboratory procedure that forces a normal cell to spit out half of its chromosome content, leaving just one set. In mammals this renders this cell equal to sperm or ...

Liver

... of the liver. Due to the ability of the liver to regenerate , both the donor and recipient end up with normal liver function if all goes well. This procedure is more controversial as it entails performing a much larger operation on the donor, and indeed there have been at least two donor deaths out of the ...

Mendelian inheritance

... also had the foresight to look through several successive generations of his pea plants and record their variations. Without his careful attention to procedure and detail, Mendel's work could not have had the impact it made on the world of genetics. Mendel's law of segregation Mendel's law of ...

Mitosis

... of a cell in the process of mitotic division. (Schfer.) I to III, prophase; IV, metaphase; V and VI, anaphase; VII and VIII, telophase. The whole procedure is very similar among most eukaryotes, with only minor variations. As prokaryotes lack a nucleus and only have a single chromosome with no ...

Monoclonal antibody

... picture below shows all these possibilities: Chimeric and humanized antibodies One problem in medical applications is that the standard procedure of producing monoclonal antibodies yields mouse antibodies, and these are rejected by the human immune system. Various approaches to overcome this ...

Reduction

... gimme. In physiology , reduction or reducing is another name for weight loss or dieting . In surgery , reduction is a medical procedure to restore a fracture to a correct alignment. ...

Mitosis

... of a cell in the process of mitotic division. (Schfer.) I to III, prophase; IV, metaphase; V and VI, anaphase; VII and VIII, telophase. The whole procedure is very similar among most eukaryotes, with only minor variations. As prokaryotes lack a nucleus and only have a single chromosome with no ...

X-ray crystallography

... To determine a structure, one must first grow crystals of the molecule of interest using some method of crystallization . This can be a painstaking procedure for macromolecules such as protein and DNA complexes. The crystals are harvested and often frozen with liquid nitrogen . Freezing crystals both ...