... method is a buccal swab .
Next, restriction fragment
length polymorphism (RFLP) analysis is performed ... has been introduced: AFLP, or amplified fragment
length polymorphism. This new technique is ... year sentences.
length polymorphism (RFLP)
Polymerase chain reaction
... template, which contains the region of the DNA fragment
to be amplified
Two primers , which ... USD 2,500 in 2004 .
The DNA fragment
to be amplified is determined by selecting ... exactly match the beginning and end of the DNA fragment
to be amplified. They anneal (adhere) to the ...
1 Sites of cleavage
complementarity and splicing
3 Restriction ... each cleave DNA by a different mechanism.
complementarity and splicing
Because ... spontaneously in a test tube. Thus, a sticky-end fragment
will readily reunite with ...
... known as the complementarity determining region 3 (CDR3).
The Fc fragment
is the stem of the "Y" and is composed from two heavy chains that each ... and light chains can be fused together to form a single chain variable fragment
(scFv), which retains the original specificity of the parent ...
... , while the other contains a carboxylic acid functional group.
of the parent molecule that was originally a carboxylate gains a hydrogen ion from the additional water molecule. The fragment
that was originally an alkyl group collects the remaining hydroxyl ...
... An Okazaki fragment
is a relatively short fragment
of DNA that is created by primase and Pol III along the lagging ...
... In polymerase chain reaction (PCR), primers are used to determine the DNA fragment
to be amplified by the PCR process. The length of primers is usually not ... nucleotides), and they match exactly the beginning and the end of the DNA fragment
to be amplified. They anneal (adhere) to the DNA template at these ...
Restriction fragment length polymorphism
In molecular biology , the term restriction fragment
length polymorphism (or RFLP ) is used in two related contexts: as a ... determine the relationships among species .
Sticky end/blunt end
... complementary region, then the DNA is said to possess an overhang :
5'- ApTpCpTpGpApCpT -3'
3'- TpApGpApCpTpGpApCpTpApCpG -5'
If another DNA fragment
exists with a complementary overhang, then these two overhangs will tend to associate with each other and each strand is said to possess a sticky ...
... form, into which life was first breathed."
"The whole history of the world, as at present known, ... will hereafter be recognised as a mere fragment
of time, compared with the ages which have elapsed since the first creature, the progenitor of innumerable extinct and living descendants, was ...
Expressed sequence tag
... and sequence determination. An EST is produced by one-shot sequencing of a cloned mRNA , and the resulting sequence is a relatively low quality fragment
whose length is limited by current technology to approximately 500 to 800 nucleotides . ESTs are also a useful resource for designing probes for ...
... used to introduce restriction enzyme sites, or to mutate (change) particular bases of DNA. PCR can also be used to determine whether a particular DNA fragment
is found in a cDNA library .
Main article: Gel electrophoresis
Gel electrophoresis is one of the principal tools ...
... other hand, can get sequence information from individual peptides by isolating them, colliding them with a nonreactive gas, and then cataloging the fragment
Proteome is also the name of a formerly independent company involved in protein functional annotation; this company has been ...
... that reliably provoke an immune response
Subunit - rather than introducing a whole inactivated or attenuated micro-organism to an immune system, a fragment
of it can create an immune response
Recombinant Vector - by combining the physiology of one micro-organism and the DNA of the other, immunity can ...