Gel electrophoresis
...
Electrofocusing
Southern
blotting
Northern
blotting
Western
blotting
External links
Biotechniques ...
Gel electrophoresis
...
Electrofocusing
Southern
blotting
Northern
blotting
Western
blotting
External links
Biotechniques ...
Molecular biology
... (PCR)
2.3 Gel electrophoresis
2.4 Western
blotting and immunochemistry
3 History
4 ... is known as an isoelectric ...
Western
blotting and immunochemistry
Main article: Western ... and preprative techniques.
In Western
blotting , proteins are first separated by size, in a thin ...
Genetic fingerprinting
... which are separated into bands during agarose gel electrophoresis . Next, the bands of DNA are transferred via a technique called Southern
blotting from the agarose gel to a nylon membrane. This is treated with a radioactively-labelled DNA probe which binds to certain and specific DNA sequences ...
Restriction fragment length polymorphism
... The restriction fragments are then separated according to length by agarose gel electrophoresis . The resulting gel may be enhanced by Southern
blotting .
Result
The distance between the locations cut by restriction enzymes (the restriction sites ) varies between individuals: so the length of ...