2.3 Gel electrophoresis
2.4 Western blotting
4 ... is known as an isoelectric ...
Main article: Western ... and preprative techniques.
In Western blotting
, proteins are first separated by size, in a thin ...
... which are separated into bands during agarose gel electrophoresis . Next, the bands of DNA are transferred via a technique called Southern blotting
from the agarose gel to a nylon membrane. This is treated with a radioactively-labelled DNA probe which binds to certain and specific DNA sequences ...
Restriction fragment length polymorphism
... The restriction fragments are then separated according to length by agarose gel electrophoresis . The resulting gel may be enhanced by Southern blotting
The distance between the locations cut by restriction enzymes (the restriction sites ) varies between individuals: so the length of ...