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West Nile Virus Screening and Diagnostics Technology Emerges In Step with North American Outbreak

e Virus IFA Slides and West Nile Virus Positive Control, which are intended for the qualitative serologic detection of antibodies to West Nile virus.

InBios offers the West Nile Detect IgM Capture ELISA. The ELISA procedure utilizes wells coated with goat anti-human IgM to which diluted samples are added. After incubation and washing, WNV derived recombinant protein (WNRA) and a control consisting of normal cell antigen are each added to two of four replicate wells of sample. Detection is by means of HRP-conjugated anti-WNV monoclonal antibody and substrate. Absorbance is measured at 450 nm. Above a certain threshold, the ratio of the absorbance of the WNRA and the antigen control wells presumptively determines whether antibodies to WNV are present. There are 3 wash steps and total incubation time is 3 hours and 16 minutes.

In addition InBios offers as analyte specific reagents: West Nile Detect IgG Elisa, and antigen and reagents for both IgM and IgG WNV ELISAs.
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