( t WNV antigen is added. Detection is b...)West Nile Virus Screening and Diagnostics Technology Emerges In Step with North American Outbreak,Health

t WNV antigen is added. Detection is by means of mouse anti-flavivirus conjugated with horseradish peroxidase (HRP), and enzyme substrate plus chromogen. Absorbance is measured at 450 nm. There are 3 wash steps and the total incubation time is 3 hours and 45 minutes. Positive samples must be tested with the background subtract procedure (either on the initial test or on a repeat test) to rule out false positives caused by rheumatoid factor and heterophilic antibodies. The IgG ELISA utilizes wells coated with recombinant WNV antigen to which diluted samples are added. Detection is by means of HRP-conjugated goat anti-human IgG, and enzyme substrate plus chromogen. Absorbance is measured at 450 nm. There are 3 wash steps and the total incubation time is 1 hour and 45 minutes.

In addition, Focus Diagnostics Reference Laboratory offers WNV testing that includes the PRNT, which satisfies the CDC requirement for performing a WNV-specific serology test; antibody ELISAs for serum and CSF, an IgA ELISA for serum, and RT-PCR to detect viral RNA in CSF or serum.

PANBIO offers the West Nile Virus IgM Capture ELISA and the West Nile Virus IgG Indirect ELISA. The IgM capture ELISA procedure utilizes wells coated with polyclonal sheep anti-human IgM antibodies to which diluted samples are added. After incubation and washing, a solution of inactivated WNV antigen combined with HRP-conjugated monoclonal anti-flavirus is added. After incubation and washing, the substrate is added. Absorbance is measured at 450 nm. There are 2 wash steps and total incubation time is 2 hours and 10 minutes. The IgG ELISA utilizes wells coated with purified and inactivated WNV antigen to which diluted samples are added. Detection is by means of HRP-conjugated sheep anti-human IgG and substrate. Absorbance is measured at 450 nm. There are 2 wash steps and total incubation time is 1 hour and 10 minutes.

In addition PANBIO offers as analyte specific reagents: Panbio West Nil
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