Navigation Links
The Past, Present and Future of HLA Typing


The Human Leukocyte Antigen (HLA) region, located on the short arm of chromosome 6 (6p21.3), is a highly polymorphic region containing about 200 genes. The HLA region is the human equivalent of the Major Histocompatibility Complex (MHC), and as such contains a set of genes that serve as the backbone of antigen presentation. The Class I proteins, classically involved in presenting endogenous antigens to CD8+ T-cells, are expressed by genes located in the HLA-A, -B and C loci. In contrast, the Class II proteins, which associate with and present exogenous antigens to CD4+ T-cells, are expressed by the HLA DR, -DQ and DP loci. Each locus is highly polymorphic; for example, there appear to be 300+ alleles in the HLA-B or DRB1 loci. A clear understanding of the differences between HLA polymorphisms has provided ample insight into why and how foreign tissue is rejected by the host, and as such been a critical enabler of the field of transplantation. Today, a variety of techniques are applied for HLA tissue typing, providing an important tool to increase the success rate of human transplants.

For many years, HLA polymorphisms were typed by serological responses HLA antigens; sera containing antibodies to Class I and II proteins were collected from multiparous women, or individuals who had received multiple blood transfusions. In addition, polymorphisms in Class II proteins were analyzed by T-cell responses in the Mixed Lymphocyte Reaction (MLR). However, the advent of recombinant DNA technology, which paved the way to identifying genetic differences among the HLA loci directly, has led many laboratories to abandon classic serological typing methods. Today, several different types of HLA DNA typing methods exist and are commonly used by clinical laboratories. In general, HLA DNA can be typed either by hybridizing labeled, sequence specific oligonucleotide probes to HLA loci amplified by the polymerase chain reaction (PCR), or by using PCR to amplify th
'"/>




Page: 1 2 3

Related medicine technology :

1. Phase 3 Results for Dabigatran Etexilate, an Investigational Oral Anticoagulant, Presented Today at the XXIst Congress of the International Society on Thrombosis and Haemostasis
2. Neose Announces Presentation of Positive Preclinical Data on GlycoPEGylated Factor VIIa at the XXI Congress of the International Society on Thrombosis and Haemostasis
3. Inspiration Biopharmaceuticals Presents Data From Hemophilia Program at International Society On Thrombosis and Haemostasis (ISTH) Annual Meeting
4. Halozyme Therapeutics Enhanze Technology Large Protein Molecule Therapeutic Clinical Trial Results Presented at the 34th Annual Meeting of the Controlled Release Society
5. Generex Biotechnology to Make Presentations At 46th Annual Meeting of the European Society of Pediatric Endocrinology
6. Auxilium Pharmaceuticals to Present at the 2007 CEUT Emerging Growth Opportunities Conference
7. ViaCell to Present at the C.E. Unterberg, Towbin Emerging Growth Opportunities Conference
8. Accelrys to Present to Investors at C.E. Unterberg Emerging Growth Opportunities Conference
9. Metabasis to Present at C.E. Unterberg, Towbin Emerging Growth Conference and Attend Pacific Growth Equities Conference
10. Inspire Announces Webcast of Presentation at C.E. Unterberg, Towbin Emerging Growth Opportunities Conference
11. Sunesis Pharmaceuticals to Present at the C. E. Unterberg, Towbin Emerging Growth Conference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The Past Present and Future HLA Typing
(Date:5/28/2015)... , May 28, 2015 Compassion & Choices ... has approved a bill that would authorize the option of ... was 5-2. ... 128) , would allow mentally competent adults with six months ... they could take to painlessly and peacefully end an unbearable ...
(Date:5/28/2015)... SANTA MONICA, Calif. , May 28, 2015 /PRNewswire-USNewswire/ ... require doctors to check a state prescription database before ... drug addiction and thousands of overdose deaths. ... D- Bell Gardens , will require prescribers ... System (CURES) before prescribing Schedule II and III drugs, ...
(Date:5/28/2015)... , May 28, 2015  The U.S. Department ... Orbit Medical Inc. and Rehab Medical Inc. to resolve ... with the filing of a whistleblower lawsuit by two ... sales representatives, under the direction of one of Orbit,s ... so that Orbit could obtain payment from Medicare for ...
Breaking Medicine Technology:California Committee Sends Medical Aid-in-Dying Bill to Senate Floor 2California Committee Sends Medical Aid-in-Dying Bill to Senate Floor 3California Senate Approves Legislation Requiring Physicians To Check State Database Before Prescribing Powerful, Addictive Medications, Says Consumer Watchdog 2Electric Wheelchair Sellers Agree To $7.5 Million Settlement Of Whistleblower Case Alleging That Company Forged Medical Records As Part Of Scheme To Defraud Medicare 2Electric Wheelchair Sellers Agree To $7.5 Million Settlement Of Whistleblower Case Alleging That Company Forged Medical Records As Part Of Scheme To Defraud Medicare 3Electric Wheelchair Sellers Agree To $7.5 Million Settlement Of Whistleblower Case Alleging That Company Forged Medical Records As Part Of Scheme To Defraud Medicare 4
(Date:5/28/2015)... Madre, CA (PRWEB) May 28, 2015 The ... superior outpatient wound center management for a decade with a ... partnership was formed between USC and Wound Care Advantage to ... was recognized for a drop in the rate ... , Wound healing is a complex undertaking that can ...
(Date:5/28/2015)... Texas (PRWEB) May 28, 2015 ... excited to announce that the International Organization for ... Integrated Domain Group (BRIDG) Model version 3.2 as ... and its link with healthcare. This standard, which ... Standard (DIS) ballot, was unanimously approved on 24 ...
(Date:5/28/2015)... May 28, 2015 Restricting access to ... violence, but policies that more clearly define alcohol misuse ... review of existing research and public policies by the ... published online April 30 in the peer-reviewed journal ... other forms of alcohol misuse in association with firearm ...
(Date:5/28/2015)... 2015 New Jersey Plastic Surgeon and ... world-class results. With over 30 years of experience ... in art, Dr. Rafizadeh knows what it takes to ... Plastic Surgery involves the principles of proportion, harmony and ... level of comfort and personalized care from the moment ...
(Date:5/28/2015)... 28, 2015 Medelis, Inc., a specialty ... the company in the role of president. , “ Bill ... the CRO industry,” said Larry Flanagan, Medelis’ CEO and co-founder. ... through our next phase of growth.” , Taaffe joined ... from nine employees to over 4,500 employees, achieving 12 consecutive ...
Breaking Medicine News(10 mins):Health News:USC Verdugo Hills Hospital Wound Care Team Provides Outstanding Healing Results 2Health News:CDISC Announces BRIDG Model for Research as Final ISO Standard 2Health News:CDISC Announces BRIDG Model for Research as Final ISO Standard 3Health News:UC Davis Research Finds That Restricting Firearms Access for People who Misuse Alcohol May Prevent Violence 2Health News:UC Davis Research Finds That Restricting Firearms Access for People who Misuse Alcohol May Prevent Violence 3Health News:UC Davis Research Finds That Restricting Firearms Access for People who Misuse Alcohol May Prevent Violence 4Health News:"NJ Top Doc", Dr. Farhad Rafizadeh is Excited to Offer New Services at his Plastic Surgery Practice 2Health News:"NJ Top Doc", Dr. Farhad Rafizadeh is Excited to Offer New Services at his Plastic Surgery Practice 3Health News:Bill Taaffe, Former President and CEO of ICON Clinical Research-US, Joins Medelis, a Specialty Oncology CRO, in the Role of President 2
... ... Direct Product Evaluations and In-depth, Confidential Interviews Conducted by KLAS with Hospital Executives and ... Chicago ... RES-Q® Healthcare Systems announced that its RES-Q® Labor Resource Management system was ranked #1 ...
... , TITUSVILLE, N.J., Sept. 9 Attention Deficit Hyperactivity ... children(1) and 4 percent of adults in the U.S.(2) Recent ... of U.S. school-aged children meet the Diagnostic and Statistical Manual of ... ADHD diagnosis.(3) Meanwhile, it is estimated that just 15 percent of ...
... SALT LAKE CITY, Sept. 9 ... reference laboratory and a leader in innovative laboratory research and ... scientist, received a prestigious National Institutes of Health (NIH) grant ... scientific officer of Integratech Proteomics, a University of Utah start-up ...
... , , , ... program may have ended, but financial incentives for improving health and ... DeKalb Surgical Associates, "Cash for Crow,s Feet" program offers ... - at half the typical cost, said Dr. David R. Fern, ...
... The Oreck vacuum was invented with women in mind, according ... to be lightweight and powerful for ease of use, effective cleaning ... our support of Susan G. Komen for the Cure in the ... to help raise donations for the cause," Oreck said. , ...
... , Leg Cramps Can be Red Flag ... 9 As many as 4.5 million American women have a common ... arterial disease. September is Peripheral Arterial Disease (P.A.D.) Awareness Month ... with Heart Disease have launched a national effort to inform Americans about ...
Cached Medicine News:Health News:RES-Q Labor Resource Management Ranked as the Top Staff Scheduling System Solution in KLAS 2009 Special Report 2Health News:CONCERTA(R) (methylphenidate HCI) LAUNCHES MOBILE WEB EXPERIENCE TO PROVIDE TOOLS AND SUPPORT FOR ADHD AWARENESS 2Health News:CONCERTA(R) (methylphenidate HCI) LAUNCHES MOBILE WEB EXPERIENCE TO PROVIDE TOOLS AND SUPPORT FOR ADHD AWARENESS 3Health News:CONCERTA(R) (methylphenidate HCI) LAUNCHES MOBILE WEB EXPERIENCE TO PROVIDE TOOLS AND SUPPORT FOR ADHD AWARENESS 4Health News:CONCERTA(R) (methylphenidate HCI) LAUNCHES MOBILE WEB EXPERIENCE TO PROVIDE TOOLS AND SUPPORT FOR ADHD AWARENESS 5Health News:ARUP Laboratories' Scientist Tanya Sandrock Receives NIH Grant to Search for Additional HIV Therapies 2Health News:'Cash For Crow's Feet' Program Offers Effective - and Affordable - Laser Skin Treatments 2Health News:New Special Edition Oreck(R) Vacuum Helps Clean for the Cure(R) 2Health News:New Special Edition Oreck(R) Vacuum Helps Clean for the Cure(R) 3Health News:WomenHeart and P.A.D. Coalition Join Forces to Educate Women about Peripheral Arterial Disease 2Health News:WomenHeart and P.A.D. Coalition Join Forces to Educate Women about Peripheral Arterial Disease 3
Heparin-Platelet Factor 4 Induced Antibody...
Multi-constituent normal and abnormal plasma controls for routine and specialty assays performed on the STA© family of hemostasis analyzers....
ASO Calibrator (Latex-Enhanced Immunoturbidimetric)...
CRP Calibrator...
Medicine Products: