Navigation Links
Point of Care Cardiac Analyzers Yield Rapid Cardiac Marker Results


Helen Londe, M.D.
Contributing Editor

Clinical guidelines from the disciplines of cardiology and laboratory and emergency medicine have all emphasized the importance of cardiac markers in diagnosing or ruling out acute myocardial infarction. The measurement of cardiac troponins is especially useful in the large fraction of patients presenting with acute coronary syndrome (ACS) who have negative 12-lead EKGs. About 30% of patients previously considered to have unstable angina are now given a diagnosis of myocardial infarction without ST-segment elevation based on their cardiac troponin levels. Troponin levels additionally aid in risk stratification.

The development of accelerated protocols for diagnosing ACS in patients with chest pain necessitates the reduction of turnaround time (TAT) for cardiac marker results. Recommendations for the optimum TAT vary from 30 to 60 minutes. With regard to TAT, quantitative point of care (POC) assays that directly assay whole blood provide an attractive alternative to central lab testing. Although the advantages of rapid TAT must be balanced with the need for stringent quality control (QC), many POC analyzers have internal QC systems, and manufacturers provide external controls appropriate to each assay.

Biosite Inc., Response Biomedical Corp., and Roche Diagnostic Systems have FDA approved point of care technology to measure cardiac markers. The indications for use are for rapid quantitative measurement of markers to aid in the diagnosis of myocardial injuries.

Biosite Inc. offers the Triage Cardiac Panel, a fluorescence immunoassay for quantitative determination of CK-MB, myoglobin and troponin I in whole blood and plasma specimens using EDTA as the anticoagulant. To perform the test, a blood sample is added to the sample port of the test device from the Triage Cardiac Panel Kit. The device contains all the reagents for the test. After red blood cells are separated from plasma via filter , the plasma moves into the reaction chamber and reacts with fluorescent-conjugated antibodies to all three markers. After incubation, the sample-reagent mixture flows through the device detection lane where complexes of analyte and conjugates are captured. The level of fluorescence is directly proportional to the concentration of the analyte. The device is read in the Triage MeterPlus, which is a multifunctional reader also utilized for other Triage assays. The test yields simultaneous results for all three markers in approximately 15 minutes. Data can be managed using Triage Census data management software and uploaded quickly to laboratory information systems. The system contains both internal and external quality controls that are user-friendly. The reader is compact and portable, with a self-contained power source.

Response Biomedical Corp. offers the RAMP Cardiac Marker System, with separate test kits for CK-MB, myoglobin and troponin I. It is also a quantitative immunofluorescence assay for EDTA whole blood specimens. To perform the test, a diluted blood sample is added to the sample well of the test cartridge, the red blood cells are retained, and the plasma migrates. Fluorescent-dyed latex particles coated with antigen specific antibodies bind to antigen in the sample. Antigen-bound particles are captured in the detection zone and excess particles are captured at the internal control zone by anti-immunoglobins. The fluorescence of the detection and internal control zones is measured by the RAMP Clinical Reader, which is a multifunctional reader utilized for the RAMP platform technology. The ratio of the values is calculated and the analyte is quantified by interpolation from a lot-specific standard curve supplied in the test kit. The tests for CK-MB and Troponin I yield results in 10 and 12 minutes respectively. Data can be uploaded quickly to laboratory information systems. The reader is compact and portable, with a self-contained p ower source.

Roche Diagnostic Systems offers The Cardiac Reader System with separate test strips for for troponin T and myoglobin. It is a quantitative gold-labeled immunochemistry assay for heparinized whole blood specimens. The strips are read in the Cardiac Reader analyzer, which measures the intensity of the signal and control line of each test strip. There is a calibration curve for each test strip lot contained in a code chip, which is utilized by the reader for conversion to a quantitative result. The tests for myoglobin and troponin T yield results in 8 and 12 minutes respectively. Data can be uploaded to laboratory information systems. The system requires an optical quality control and quantitative marker control solutions. The reader is compact and portable, with a self-contained power source.
'"/>




Related medicine technology :

1. Point of Care Strep Tests Speed Treatment, Lower Costs
2. Analyzers Offer Point-of-Care Lipid Panels
3. Hemoglobin Testing at the Point of Care
4. New Point-of-Care Methods to Measure Coronary and Cerebrovascular Risk
5. Blood Gas Analysis: The Pros and Cons of Point-Of-Care Testing
6. Dade Behrings (hsCRP) Tests and BNTM Series Analyzers
7. Hematology Analyzers Simple, Sophisticated Technology Serving All Patients
8. Urinalysis Analyzers: Large Capabilities in Every Size Device
9. Rapid Whole Blood Testing for Infectious Mononucleosis Antibodies
10. Rapid Flu Tests May Prove Especially Helpful This Season
11. Avian (Bird) Flu: Is There a Role for Rapid Tests?

Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2016)... 24, 2016  Arkis BioSciences, a leading innovator ... more durable cerebrospinal fluid treatments, today announced it ... funding is led by Innova Memphis, followed by ... private investors.  Arkis, new financing will accelerate the ... market release of its in-licensed Endexo® technology. ...
(Date:6/23/2016)... DUBLIN , June 23, 2016 ... "Dialysis Devices Global Market - Forecast to 2022" report ... is the treatment method for the patients with kidney failure, ... and excess fluid from the patient,s blood and thus the ... sodium, potassium and chloride in balance. Increasing ...
(Date:6/23/2016)... 23, 2016 Research and Markets ... Market by Type (Organic Chemical (Sugar, Petrochemical, Glycerin), Inorganic ... Coating, Parenteral) - Global Forecast to 2021" report ... The global pharmaceutical excipients market is projected to ... of 6.1% in the forecast period 2016 to 2021. ...
Breaking Medicine Technology:
(Date:6/26/2016)... ... June 27, 2016 , ... Quality metrics are proliferating in cancer care, and ... in the eye of the beholder, according to experts who offered insights and commentary ... of Managed Care. For the full issue, click here . , For the ...
(Date:6/26/2016)... ... June 26, 2016 , ... Many women are ... with endometriosis. These women need a treatment plan to not only alleviate symptoms ... can help for preservation of fertility and ultimately achieving a pregnancy. The specialists ...
(Date:6/25/2016)... ... June 25, 2016 , ... First Choice Emergency Room , the largest ... as the Medical Director of its new Mesquite-Samuell Farm facility. , “We are ... Mesquite location,” said Dr. James M. Muzzarelli, Executive Medical Director of First Choice Emergency ...
(Date:6/24/2016)... ... June 24, 2016 , ... A recent article published ... unfamiliar with. The article goes on to state that individuals are now more comfortable ... less common operations such as calf and cheek reduction. The Los Angeles area medical ...
(Date:6/24/2016)... ... June 24, 2016 , ... Global law firm Greenberg Traurig, P.A. announced that ... chosen by their peers for this recognition are considered among the top 2 percent ... special honors as members of this year’s Legal Elite Hall of Fame: Miami ...
Breaking Medicine News(10 mins):