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Breast Cancer and HER-2/neu: Diagnostic Tools for Targeted Therapy

ciated with Her-2/neu. An excess of Her-2/neu probe fluorescence over control probe fluorescence indicates Her-2/neu amplification. Challenges of FISH include higher cost and the need for a fluorescent microscope. One commercially available kit is the Ventana INFORM, which works in concert with the IHC system mentioned above. Other FDA-approved kits are PathVysion (Abbott-Vysis) and the Her-2 FISH PharmDx kit (Dako USA).

ELISA is also available to quantify Her-2/neu expression. In contrast to IHC and FISH, ELISA can detect Her-2/neu as a serum marker. This bypasses the need for a tissue specimen and may allow earlier Her-2/neu detection. The Her-2/neu ELISA (Bayer Diagnostics Oncogene Science) is a sandwich-type immunoassay approved to monitor patients with known metastatic breast cancer.

With other methods such as RT-PCR on the horizon, one can expect further evolution of the diagnostic and therapeutic landscape. Along with it comes the promise of similar solutions for other cancer patients.
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