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Living cell-based assays for protein function 65
Companies developing cell-based protein assays 65
Protein function studies .. 66
Transcriptionally Active PCR . 66
Protein-protein interactions .. 66
Yeast two-hybrid system . 68
Membrane one-hybrid method .. 69
Protein affinity chromatography 69
Phage display . 69
Fluorescence Resonance Energy Transfer .. 70
Bioluminescence Resonance Energy Transfer 70
Detection Enhanced Ubiquitin Split Protein Sensor technology 70
Protein-fragment complementation system .. 70
In vivo study of protein-protein interactions . 71
Computational prediction of interactions 71
Interactome 72
Protein-protein interactions and drug discovery .. 73
Companies with technologies for protein-protein interaction studies . 73
Protein-DNA interaction . 74
Determination of protein structure 74
X-Ray crystallography 75
Nuclear magnetic resonance . 76
Electron spin resonance . 76
Prediction of protein structure . 76
Protein tomography . 77
X-ray scattering-based method for determining the structure of proteins .. 78
Prediction of protein function .. 78
Three-dimensional proteomics for determination of function .. 78
An algorithm for genome-wide prediction of protein function . 79
Monitoring protein function by expression profiling 79
Isotope-coded affinity tag peptide labeling .. 79
Differential Proteomic Panning 80
Cell map proteomics . 80
Topological proteomics 81
Organelle or subcellular proteomics . 82
Nucleolar proteomics 82 - 5 -
Glycoproteomic technologies 82
High-sensitivity glycoprotein analysis 83
Fluorescent in vivo imaging of glycoproteins 83
Integrated approaches for protein characterization . 83
Imaging ma
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