Navigation Links
Packaging therapeutic RNAs for targeted treatment of breast cancer
Date:4/18/2012

is ensuring that the siRNAs get to where they need to go. "Naked siRNAs are cleared by the kidneys very quickly, and cells don't take them up readily," Lieberman explained. "Other delivery systems, like liposomes, tend to make siRNAs collect in the liver.

"The challenge is to deliver the siRNAs to the site of the tumor," she added, "without creating toxicity or inflammation in other portions of the body."

Lieberman and Song decided to adapt a targeting method they had originally developed for eliminating HIV-infected cells to transport siRNAs to breast tumors. First they bound siRNAs that would turn off a gene called PLK1 (an enzyme that promotes cell division) to protamine, a peptide that shrink-wraps DNA in sperm cells. Protamine was attached to an antibody fragment (called an ScFv) against HER2, creating a delivery package addressed directly to breast cancer cells with HER2 on their surface.

"The protamine stabilizes the siRNAs and protects them from being degraded by enzymes in the blood stream," Lieberman said. "And the ScFv antibody fragment makes sure that they only get delivered to and act in HER2-positive cells."

The team administered both naked and ScFv-protamine packaged siRNAs against PLK1 to mice with human breast tumors. While the naked siRNAs quickly accumulated in the kidneys and were excreted—their half life within the mice was a mere six minutes—Lieberman and Song found that the packaged ones were delivered directly to the HER2-positive tumor sites within the mice. There they were readily detectable for at least 72 hours.

Once in the tumors, the siRNAs were absorbed into tumor cells, where they turned off PLK1, retarding tumor growth and suppressing metastasis compared to untreated controls or those that received naked siRNAs.

The team found no evidence of inflammation or toxicity in the liver, kidneys, or bloodstream, indicating that the siRNAs were silencing PLK1
'/>"/>

SOURCE Boston Children's Hospital
Copyright©2010 PR Newswire.
All rights reserved

Page: 1 2 3 4

Related medicine technology :

1. Sigma-Aldrich Enhances Asia-Pacific Customer Support With New Distribution and Packaging Capabilities in India
2. Intercept Technology Packaging Advanced Training Program Hosted by Liberty Packaging
3. Cardinal Health Unveils New Unit-dose, Barcoded Packaging Solutions for Oral Liquid Medications
4. Novation Extends Unit Dose Repackaging and Bar Coding Contract with Safecor Health
5. Pharmaceutical Packaging Industry - 2011 Yearbook
6. Honeywell Introduces New Ultra-High Barrier Film for Pharmaceutical Packaging
7. Global Markets for Contract Pharmaceutical Manufacturing, Research And Packaging: Focus on Emerging Markets
8. Contract Pharmaceutical Manufacturing, Research and Packaging: Global Markets
9. Global Markets for Contract Pharmaceutical Manufacturing, Research and Packaging: Focus on North America
10. Global Markets for Contract Pharmaceutical Manufacturing, Research and Packaging: Focus on Europe
11. Sherpa Clinical Packaging Completes New Production Facility
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/22/2014)... WASHINGTON and SHANGHAI ... Healthcare Group (BHG), a global knowledge leader in ... strategic partnership to support healthcare innovation in ... for personal connected health technologies. Continua and BHG ... Healthcare Forum being held this week in ...
(Date:9/22/2014)... , Sept. 22, 2014  Aethlon Medical, ... therapeutic devices to address infectious disease, cancer and ... Advanced Research Projects Agency (DARPA) has informed the ... to proceed with year four of a five-year ... on September 30, 2011 under DARPA,s Dialysis-Like Therapeutics ...
(Date:9/22/2014)... , Sept. 22, 2014 ... of the "Global Ablation Technologies (Radiofrequency, Ultrasound, ... 2019" report to their offering. ... the global ablation technologies market has been divided ... ultrasound, microwave, cryotherapy, hydrothermal, and hydromechanical ablation technologies. ...
Breaking Medicine Technology:Continua and Borderless Healthcare Group Form Strategic Partnership, Launch the Healthcare Technology Innovation Center in Shanghai 2Continua and Borderless Healthcare Group Form Strategic Partnership, Launch the Healthcare Technology Innovation Center in Shanghai 3Continua and Borderless Healthcare Group Form Strategic Partnership, Launch the Healthcare Technology Innovation Center in Shanghai 4Aethlon Medical Receives Notice of DARPA Contract Renewal 2Aethlon Medical Receives Notice of DARPA Contract Renewal 3Aethlon Medical Receives Notice of DARPA Contract Renewal 4Global Ablation Technologies (Radiofrequency, Ultrasound, Irreversible Electroporation, Cryotherapy, Microwave) Market - Forecasts to 2019 2Global Ablation Technologies (Radiofrequency, Ultrasound, Irreversible Electroporation, Cryotherapy, Microwave) Market - Forecasts to 2019 3
... 5, 2012  InterMune, Inc. (NASDAQ: ITMN ) ... the fourth quarter ended December 31, 2011, as well ... Esbriet therapy in Germany during 2011.   InterMune ... including new efficacy data from the RECAP extension study ...
... TUCSON, Ariz. and PROVIDENCE, R.I., Jan. 5, 2012 ... it has awarded $1.0 million to Tivorsan Pharmaceuticals ... filing of an Investigational New Drug (IND) application with ... recombinant humanized form of biglycan as a potential muscular ...
Cached Medicine Technology:InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 2InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 3InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 4InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 5InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 6InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 7InterMune Reports Preliminary Fourth Quarter 2011 Esbriet® (pirfenidone) Revenue and Other Recent Business Highlights 8MDA Awards $1 Million to Tivorsan Pharmaceuticals for Accelerating Pivotal Pre-Clinical Work on TVN-102 as a Potential Muscular Dystrophy Treatment 2MDA Awards $1 Million to Tivorsan Pharmaceuticals for Accelerating Pivotal Pre-Clinical Work on TVN-102 as a Potential Muscular Dystrophy Treatment 3MDA Awards $1 Million to Tivorsan Pharmaceuticals for Accelerating Pivotal Pre-Clinical Work on TVN-102 as a Potential Muscular Dystrophy Treatment 4
(Date:9/22/2014)... York, NY (PRWEB) September 22, 2014 ... pain on the side of the hip , which ... bursitis treatment in Manhattan. This treatment is now offered at ... which is also called trochanteric bursitis, occurs when the ... a fluid-filled sac in the hip, between the iliotibial ...
(Date:9/22/2014)... The concept of a whole eye transplant seems ... grant from the U.S. Department of Defense, researchers ... Medicine hope to someday make implantation of an ... eye transplant could be a holy grail for ... of ophthalmology and grant co-recipient with colleagues at ...
(Date:9/22/2014)... New York, US (PRWEB) September 22, 2014 ... Heartbeat Experts’ perspectives on stakeholder management needs for the ... speak with Heartbeat Experts on stakeholder strategies for any ... clinically meaningful points of differentiation within an increasingly crowded ... , The impact of identifying community-based thought ...
(Date:9/22/2014)... Va. (PRWEB) September 22, 2014 ... the nation’s leading nonprofit authority on the ... healthcare information exchange, announced findings from its recent ... Health Plan Identifier (HPID) within electronic transactions adopted ... (HIPAA). WEDI conducted the survey from August 20 ...
(Date:9/22/2014)... CareFlight Air & Mobile Services ... a new AS365 N3+ Dauphin, the latest version of ... which is based at Miami Valley Hospital ... air medical transport helicopters. , “We have a lot ... Health Vice President, Emergency, Trauma, and CareFlight. “We’ve been ...
Breaking Medicine News(10 mins):Health News:DNR Clinic Offers Hip Bursitis Treatment in Manhattan 2Health News:DNR Clinic Offers Hip Bursitis Treatment in Manhattan 3Health News:Looking ahead: Whole eye transplant under development 2Health News:Download HeartBeat Experts White Paper: Stakeholder Management for new Prostate Cancer Products 2Health News:WEDI Announces Survey Results from Unique Health Plan Identifier (HPID) Industry Poll 2Health News:WEDI Announces Survey Results from Unique Health Plan Identifier (HPID) Industry Poll 3Health News:Miami Valley Hospital CareFlight begins Service with AS365 N3+ 2Health News:Miami Valley Hospital CareFlight begins Service with AS365 N3+ 3
... MDAn original and progressive report on health information ... online today, makes ground-breaking recommendations for new ... tensions that currently mar relationships between many HIT ... indemnity and error management of HIT systems. In ...
... November 11, 2010 A new study from researchers in ... more likely to undergo a screening colonoscopy than those who ... for female endoscopists seem to be common among women. In ... notion that rates of screening colonoscopy can be increased by ...
... , THURSDAY, Nov. 11 (HealthDay News) -- U.S. ... to binge-drink, smoke cigarettes or use illicit drugs, a new ... likely to abuse prescription drugs, according to the Center ... from an analysis of 2002 to 2009 data from the ...
... in French . , Montreal, November 11, 2010 ... additional cardiac test when undergoing diagnosis for potential heart problems, ... du Qubec Montral and the Montreal Heart Institute. ... Journal of Cardiopulmonary Rehabilitation and Prevention , a large sample ...
... By Jenifer Goodwin HealthDay Reporter , ... synthetic marijuana known as "K2" is sending young people ... and hallucinations, toxicologists warn. In recent months, physicians ... up in emergency rooms after smoking synthetic marijuana. Despite ...
... chemist Dr Guillaume Lessene has been awarded the Walter and ... annual general meeting in Melbourne, Australia. The $AUD150,000 ... played in shaping the Walter and Eliza Hall Institute. It ... Burgh in August at the age of 94. ...
Cached Medicine News:Health News:Fortify HIT contracts with education and ethics to protect patient safety, say informatics experts 2Health News:Fortify HIT contracts with education and ethics to protect patient safety, say informatics experts 3Health News:Screening colonoscopy rates are not increased when women are offered a female endoscopist 2Health News:Screening colonoscopy rates are not increased when women are offered a female endoscopist 3Health News:Screening colonoscopy rates are not increased when women are offered a female endoscopist 4Health News:Additional cardiac testing vital for patients with anxiety and depression 2Health News:'Fake Marijuana' Users Showing Up in Emergency Rooms 2Health News:'Fake Marijuana' Users Showing Up in Emergency Rooms 3Health News:Medicinal chemist wins inaugural De Burgh Fellowship 2
... For enzyme immunoassays, designed to meet the ... and allows the user to run set ... The floating manifold on this strip washer ... at a time. Reliable, compact, and self-contained, ...
... available to handle multiple assays: ... Embla 384 Cell washer has ... optimized to avoid cell disturbance. ... and have been integrated with ...
... miniGene LW is a new plate washing system ... and 384 channel microplates with flat, V-shaped or ... 8 or 16 channel nozzle heads. Simple LCD ... An optional plate stacker is available for the ...
Space-saving counter top model,User initiated manual defrost,Standard features (see above),Internal freezing compartment,Removable half-shelf in refrigerator,Two shelves in door,UL 471 Commercial Ref...
Medicine Products: