Navigation Links
Packaging therapeutic RNAs for targeted treatment of breast cancer
Date:4/18/2012

is ensuring that the siRNAs get to where they need to go. "Naked siRNAs are cleared by the kidneys very quickly, and cells don't take them up readily," Lieberman explained. "Other delivery systems, like liposomes, tend to make siRNAs collect in the liver.

"The challenge is to deliver the siRNAs to the site of the tumor," she added, "without creating toxicity or inflammation in other portions of the body."

Lieberman and Song decided to adapt a targeting method they had originally developed for eliminating HIV-infected cells to transport siRNAs to breast tumors. First they bound siRNAs that would turn off a gene called PLK1 (an enzyme that promotes cell division) to protamine, a peptide that shrink-wraps DNA in sperm cells. Protamine was attached to an antibody fragment (called an ScFv) against HER2, creating a delivery package addressed directly to breast cancer cells with HER2 on their surface.

"The protamine stabilizes the siRNAs and protects them from being degraded by enzymes in the blood stream," Lieberman said. "And the ScFv antibody fragment makes sure that they only get delivered to and act in HER2-positive cells."

The team administered both naked and ScFv-protamine packaged siRNAs against PLK1 to mice with human breast tumors. While the naked siRNAs quickly accumulated in the kidneys and were excreted—their half life within the mice was a mere six minutes—Lieberman and Song found that the packaged ones were delivered directly to the HER2-positive tumor sites within the mice. There they were readily detectable for at least 72 hours.

Once in the tumors, the siRNAs were absorbed into tumor cells, where they turned off PLK1, retarding tumor growth and suppressing metastasis compared to untreated controls or those that received naked siRNAs.

The team found no evidence of inflammation or toxicity in the liver, kidneys, or bloodstream, indicating that the siRNAs were silencing PLK1
'/>"/>

SOURCE Boston Children's Hospital
Copyright©2010 PR Newswire.
All rights reserved

Page: 1 2 3 4

Related medicine technology :

1. Sigma-Aldrich Enhances Asia-Pacific Customer Support With New Distribution and Packaging Capabilities in India
2. Intercept Technology Packaging Advanced Training Program Hosted by Liberty Packaging
3. Cardinal Health Unveils New Unit-dose, Barcoded Packaging Solutions for Oral Liquid Medications
4. Novation Extends Unit Dose Repackaging and Bar Coding Contract with Safecor Health
5. Pharmaceutical Packaging Industry - 2011 Yearbook
6. Honeywell Introduces New Ultra-High Barrier Film for Pharmaceutical Packaging
7. Global Markets for Contract Pharmaceutical Manufacturing, Research And Packaging: Focus on Emerging Markets
8. Contract Pharmaceutical Manufacturing, Research and Packaging: Global Markets
9. Global Markets for Contract Pharmaceutical Manufacturing, Research and Packaging: Focus on North America
10. Global Markets for Contract Pharmaceutical Manufacturing, Research and Packaging: Focus on Europe
11. Sherpa Clinical Packaging Completes New Production Facility
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/31/2015)... , July 31, 2015  Physicians and payers ... the biopharmaceutical market. Patients are better informed and ... and switching therapies. This shift in power is ... efforts toward patient-focused marketing. As part ... have learned it,s important to educate, communicate and ...
(Date:7/31/2015)... CHAPEL HILL, N.C. , July 31, 2015 /PRNewswire/ ... needs of customers who are increasingly mobile, increasingly connected, ... support a growing variety of customer interaction channels and ... of technology, including social media. According ... of the over the counter (OTC) segment in a ...
(Date:7/31/2015)... YORK , July 31, 2015  Seeger Weiss LLP ... Administration (FDA) issued a warning letter to C.R. Bard, the ... not taking adequate strides to correct violations the agency found ... of these violations after the FDA cited them during Inspectional ... 2015, in Bard,s Tempe, AZ , location ...
Breaking Medicine Technology:Consumers Forcing Pharmaceutical Industry to Shift its Marketing Focus from Products to Patients 2Best Practices in Building World-Class Consumer & Over-the-Counter Call Centers 2Seeger Weiss LLP Reports That The FDA Has Issued A Warning Letter To IVC Filter Manufacturer, C.R. Bard 2Seeger Weiss LLP Reports That The FDA Has Issued A Warning Letter To IVC Filter Manufacturer, C.R. Bard 3
... 2010 MAP Pharmaceuticals, Inc. (Nasdaq: MAPP ) ... trial.  The Company has now completed the final trial necessary ... plans to submit an NDA in the first half of ... has completed Phase 3 clinical development for the acute treatment ...
... Inc. today announced $3 million in Convertible Note funding. ... Phase II trials of BPX-101, the company,s DeCIDe™ vaccine ... a cell therapy safety switch under initial development in ... The new funding brings the total raised to date ...
Cached Medicine Technology:MAP Pharmaceuticals Announces Completion of LEVADEX™ Open-Label Safety Trial 2MAP Pharmaceuticals Announces Completion of LEVADEX™ Open-Label Safety Trial 3Bellicum Pharmaceuticals Announces $3 Million in Convertible Note Funding 2
(Date:8/1/2015)... (PRWEB) , ... August 01, 2015 , ... The condition ... among both men and women often causing pain and discomfort. Those who spend a ... circulatory problem, and Northeast Houston Vein Center is doing what it can to create ...
(Date:8/1/2015)... ... 2015 , ... Tina Wilcoxson, owner of Freeport Maine’s Royal River Natural Foods, ... the National Retail Federation, the largest retail trade association in the world, for her ... owners to be named as such. According to the NRF’s website, Retail Champions are ...
(Date:8/1/2015)... Uniondale, NY and Greenwich, CT (PRWEB) , ... ... ... Therapy (“Professional”), a Great Point Partners I, L.P. (“GPP”) portfolio company has acquired ... and Connecticut — incorporating it into its portfolio of state-of-the-art clinics. , “Our ...
(Date:8/1/2015)... ... , ... Lume Wellness creators Alex, Robert, and Sam have announced update 3.0.1 ... , The app is designed to track various metrics of wellness, including physical ... personal health and use this information for positive behavior modification. Lume Wellness keeps track ...
(Date:8/1/2015)... ... August 01, 2015 , ... “ reTXT ” was featured on NewsWatch ... coolest applications on the market for iOS, Android, and Windows. Joe Toohey, the host ... users to clarify, edit and delete any message including ones already sent. , While ...
Breaking Medicine News(10 mins):Health News:Northeast Houston Vein Center Warns Men about Varicose Veins 2Health News:Northeast Houston Vein Center Warns Men about Varicose Veins 3Health News:Local Retail Advocate Wins National Recognition 2Health News:Professional Physical Therapy Now Largest Outpatient Physical Therapy Provider in Northeast With Acquisition of Premier Physical Therapy & Wellness 2Health News:Lume Wellness Launches Health and Fitness Tracker iPhone App 2Health News:Correct Auto-Correct and Clarify the Unclear with reTXT Messaging App 2
... Move Increases Company,s Focus on Skilled Geriatric Home Health ... (Nasdaq: GTIV ), a leading provider of ... entered into an Asset Purchase Agreement with Andventure Inc., ... the sale of six Gentiva branch offices in four ...
... Briefing on Thursday, 26 February 2009 LONDON, Feb. ... & Sullivan is pleased to announce its ... Impacting the Chemicals and Materials Market to be ... p.m. GMT.(Logo: http://www.newscom.com/cgi-bin/prnh/20081117/FSLOGO )Medical applications represent ...
... Breaches with Phishing, Malware and Botnets Continuing to Thwart 2009 Security ... ... -- Offering real-time practical insights on the ever-changing security threat landscape, ... formally unveiled its blog, Optimal Security , in conjunction with ...
... , ... Laguna Beach, CA (PRWEB) February 17, 2009 -- ... health advisory service to south Orange County California residents today. Crossover ... advisory services, which are designed to provide members with a personal ...
... Sharing and Advancing Medical KnowledgeSAN FRANCISCO, Feb. 17 ... the beta version of a technology platform for the ... School of Medicine, Berkeley School of Public Health, ... global health organizations, are contributing in various ways to ...
... carcinoma (HCC) is a highly aggressive form of liver ... worldwide. Devanand Sarkar and colleagues, at Virginia Commonwealth University ... that is expressed at high levels in human HCC ... progression. They therefore suggest that targeting this gene (AEG1), ...
Cached Medicine News:Health News:Gentiva(R) Health Services Sells Selected Pediatric Home Health Care Branches 2Health News:Gentiva(R) Health Services Sells Selected Pediatric Home Health Care Branches 3Health News:Gentiva(R) Health Services Sells Selected Pediatric Home Health Care Branches 4Health News:Frost & Sullivan Helps You Know Your End-Market: Medical Trends Impacting the Chemicals and Materials Markets 2Health News:Frost & Sullivan Helps You Know Your End-Market: Medical Trends Impacting the Chemicals and Materials Markets 3Health News:Lumension Releases Annual Cybersecurity Report in Tandem with "Optimal Security" Blog Launch 2Health News:Lumension Releases Annual Cybersecurity Report in Tandem with "Optimal Security" Blog Launch 3Health News:Lumension Releases Annual Cybersecurity Report in Tandem with "Optimal Security" Blog Launch 4Health News:Lumension Releases Annual Cybersecurity Report in Tandem with "Optimal Security" Blog Launch 5Health News:Crossover Health Launches Personal Health Advisory Service 2Health News:Crossover Health Launches Personal Health Advisory Service 3Health News:Photos: Medpedia Launches 2Health News:Photos: Medpedia Launches 3Health News:Photos: Medpedia Launches 4Health News:Photos: Medpedia Launches 5
Round solid 15 mm wide lower plate. Open upper plate with inside diameter 12 mm. Serrated handle with locking thumb screw and polished finish....
Round solid 10 mm wide lower plate. Open upper plate with inside diameter 8 mm. Serrated handle with locking thumb screw and polished finish. Most popular size or model....
The popular E2002 Utrata Capsulorhexis Forceps with fine angled sharp tips. Angled 12 mm shafts with an iris stop. Flat handle with dull finish....
Fine angled tips ideal for grasping delicate capsule. Angled 12 mm shafts with iris stop allows ease of movement in the anterior chamber. Flat handle with dull finish. Manufactured in titanium....
Medicine Products: