Navigation Links
New Insight Into the Controls on a Go-To Enzyme
Date:11/19/2008

he calpastatin molecule attach to calpain in the inhibition process," said Green, the report's senior author. "However, there was no overall picture of calpastatin that revealed how it was so precise in its attachment and potent in its function."

To obtain that overall picture, St. Jude researchers used the analytical technique of X-ray crystallography, with help from nuclear magnetic resonance (NMR) spectroscopy. In this widely used method of determining protein structure, researchers first crystallize a protein to be studied. Then, they direct X-rays through the crystal and deduce the protein structure from the diffraction pattern of those X-rays. To overcome the crystallization bottleneck, a lengthy and unpredictable variable in X-ray crystallography, the investigators used NMR spectroscopy to tailor the perfect enzyme-inhibitor complex.

Tudor Moldoveanu, Ph.D., a postdoctoral fellow in Green's laboratory, performed X-ray structural analysis on such a protein crystal that consisted of a critical part of the calpastatin molecule attached to calpain. The structural picture obtained of the two proteins clutched together clearly revealed why calpastatin so specifically attaches to calpain.

"Calpain has multiple domains, and what we saw was that calpastatin wraps itself around pretty much every domain of calpain," said Moldoveanu, the report's first author. This attachment not only blocks the portion of the enzyme called the active site, where calpain performs its snipping function, but also covers regions away from that site. Such a broad molecular embrace guarantees that calpastatin will potently and rapidly shut down calpain's function, Moldoveanu said. This broad embrace also guarantees that calpastatin will precisely recognize only calpains, rather than mistakenly attach to other similar enzymes in the cell.

Furthermore, the researchers discovered how calpastatin evades being che
'/>"/>

SOURCE St. Jude Children's Research Hospital
Copyright©2008 PR Newswire.
All rights reserved

Page: 1 2 3

Related medicine technology :

1. St. Jude Study Gives New Insights Into How Cells Accessorize Their Proteins
2. Nemours Center for Childhood Cancer Research Team at the Alfred I. duPont Hospital for Children Identifies the Function of a Biomarker Present in Prostate Cancer Cells: New Insight into Therapeutic Benefit for Patients with Advanced Prostate Cancer
3. New Data Offers Insights Into the Potential Role for TYKERB(R) (Lapatinib) in Non-Cytotoxic Chemotherapy-Based Treatment Regimens
4. Apieron, Inc. Begins Customer Shipments of the Insight(TM) eNO System
5. Genmab A/S - Company Announcement: Novel Insights into HuMax-EGFr Mechanisms of Action Published in PNAS
6. Not Only Lipids and Inflammation: Insight Into a New Cause of Heart Attack and Other Vascular Disease
7. PDHI Launches ConXus Rewards, Announces Partnership With Hallmark Insights to Support Incentive-Driven Wellness Programs
8. Chemstar Corporation Announces a New Powerful, One-Step Product That Effectively Controls Listeria, Salmonella, E. coli and Fly Larvae in Floor Drains
9. RAD001 Combined with Sandostatin LAR(R) Depot and as Monotherapy Controls Growth of Rare Pancreatic Neuroendocrine Tumors
10. Enobia Pharma Initiates Clinical Testing of Enzyme Replacement Therapy to Treat Hypophosphatasia
11. Genzyme and Isis Begin Second Phase 3 Trial Of Mipomersen
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/1/2015)... May 1, 2015  Thoratec Corporation (NASDAQ: THOR ), ... support and restore failing hearts, announced today that Marta ... of President, International. This new Europe ... outside of North America , including ... Latin America , and reports directly to ...
(Date:5/1/2015)... 1, 2015 Braeburn Pharmaceuticals announced ... therapeutic focus beyond addiction and pain to ... Braeburn has acquired an implantable, six-month formulation ... for treatment of schizophrenia from Endo Pharmaceuticals. ... rights to other potential applications of the ...
(Date:5/1/2015)... SUNNYVALE, Calif., May 1, 2015 Pharmacyclics, Inc. ... results for the quarter ended March 31, 2015, as ... merger with AbbVie Inc., no conference call will be held. ... the quarter ended March 31, 2015 increased to $206 ... 31, 2014. , Worldwide IMBRUVICA ® (ibrutinib) ...
Breaking Medicine Technology:Thoratec Announces Addition To Senior Management Team 2Thoratec Announces Addition To Senior Management Team 3Braeburn Pharmaceuticals expands pipeline to include two schizophrenia treatments 2Braeburn Pharmaceuticals expands pipeline to include two schizophrenia treatments 3Braeburn Pharmaceuticals expands pipeline to include two schizophrenia treatments 4Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 2Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 3Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 4Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 5Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 6Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 7Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 8Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 9Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 10Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 11Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 12Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 13Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 14Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 15Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 16Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 17Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 18Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 19Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 20Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 21Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 22Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 23Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 24Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 25Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 26Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 27Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 28Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 29Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 30Pharmacyclics Reports First Quarter 2015 Financial Results and Provides Business Updates 31
... Following is a statement by Linda A. Suydam, D.P.A., president ... of the National Institute on Drug Abuse,s (NIDA,s) Monitoring the ... the Future survey speak to the need for continued efforts ... to talk to their teens about medicine abuse. The Consumer ...
... , DUBLIN, Ohio, Dec. 14 Cardinal Health ... the following events: , Goldman Sachs CEO Unplugged Conference ... Conference Center in New York. 28th Annual J.P. Morgan ... Westin St. Francis Hotel in San Francisco. , At these ...
Cached Medicine Technology:Statement From the Consumer Healthcare Products Association(CHPA) on Annual Monitoring the Future Survey on Drug Abuse 2Statement From the Consumer Healthcare Products Association(CHPA) on Annual Monitoring the Future Survey on Drug Abuse 3Cardinal Health Executives to Speak at Two Investor Events in January 2
(Date:5/3/2015)... 03, 2015 Indiana Fiber Network, LLC ... Bank Computer Services, Inc (BCS) has selected IFN as ... states, “The technology, service, and working relationship with IFN ... the local feel you get with IFN. I ... IFN makes local decisions and operates as a local ...
(Date:5/3/2015)... May 03, 2015 Looking for ... will directly impact your career? The Master of ... dynamic leaders in multinational companies and for professionals ... a changing global landscape, differences in culture, religion, ... The EMIB program develops the global competence and ...
(Date:5/3/2015)... SpineFrontier, a Less Exposure Surgery (LES) ... produce and market their new sacroiliac joint fusion screw, ... outpatient settings and is compatible with both lateral and ... been extensively researched and refined by SpineFrontier, the first ... approach in conjunction with a sacroiliac joint fusion system ...
(Date:5/3/2015)... Burnsville MN (PRWEB) May 03, 2015 ... in workplace safety solutions, announces its appearance at the ... Health Conference. Products that HealthPostures will exhibit at the ... Largest of the sit stand solutions is the 6100 ... push button height control, an adjustable keyboard tray, extended ...
(Date:5/2/2015)... (PRWEB) May 02, 2015 Ticket Down has ... for the long awaited event today, Saturday, May 2nd ... Diehard boxing fans will be able to secure tickets for ... The fight that boxing fans have wanted for more than ... ago. Floyd “Money” Mayweather will step inside the ring to ...
Breaking Medicine News(10 mins):Health News:Bank Computer Services, Inc Selects Indiana Fiber Network, LLC as Fiber Transport Provider 2Health News:Bank Computer Services, Inc Selects Indiana Fiber Network, LLC as Fiber Transport Provider 3Health News:The Saint Louis University's Master of International Business Is Now Enrolling For Fall 2015 2Health News:HealthPostures to Appear at the Minnesota Safety and Health Conference 2Health News:HealthPostures to Appear at the Minnesota Safety and Health Conference 3Health News:Cheap Floyd Mayweather vs. Manny Pacquiao Tickets: Ticket Down Has Last Minute Tickets for Mayweather vs. Pacquiao at the MGM Grand Garden Arena in Las Vegas 2Health News:Cheap Floyd Mayweather vs. Manny Pacquiao Tickets: Ticket Down Has Last Minute Tickets for Mayweather vs. Pacquiao at the MGM Grand Garden Arena in Las Vegas 3
Shiv Sena on Sunday strongly reacted to the recent Union Health Ministry notice to yoga guru Baba Ramdev,// saying the government is compromising national interests in connivance with multinational co...
... Atlas released early December in South Africa confirms that ... India in the pre-diabetic phase (Impaired Glucose Tolerance, IGT). ... that began in 2000, begins with the preamble: "With ... increasing rate, the prevalence of diabetes is predicted to ...
... from the Institute Pasteur has recently shown that the ... host's fat cells. //This formidable pathogen is protected against ... which it may remain dormant for years. ... on possible strategies for fighting tuberculosis. Attempts to eradicate ...
... An enzyme found at elevated levels in several human ... fruit flies//, a discovery that provides a new model ... cancer, according to researchers. ,Using fluorescent staining and ... in a gene called aurora-A to observe how changes ...
... collaborators have found that the genetically engineered bacteria that ... set the stage for a new, biobased method of ... toothpaste, mouthwash and other products. ,Xylitol is ... to some degree by the human body. It is ...
... the Department of Health plans, school girls as young ... disease, which can cause cervical cancer//. Researchers have found ... the key causes of cervical cancer, which kills around ... be immunized against the human papilloma virus (HPV) before ...
Cached Medicine News:Health News:Tuberculosis: The Bacillus Takes Refuge in Adipose Cells 2Health News:Run Amok Enzyme Causes Same Problems in Both Humans and Fruit Flies 2
The opaque outer circle will white-out even the darkest iris, making your pupil the only spot of black in a sea of white....
This lens gives the appearance of mystery and mayhem behind the blackest of eyes....
Soft silicone brush tip allows atraumatic brushing and manipulation of retina surface. Silicone brush extends 3mm beyond end of cannula. Luer lock hub for easy, secure connection to a backflush handl...
Healon vitreous aspirating cannula, 21 gauge....
Medicine Products: