Navigation Links
New Insight Into the Controls on a Go-To Enzyme
Date:11/19/2008

he calpastatin molecule attach to calpain in the inhibition process," said Green, the report's senior author. "However, there was no overall picture of calpastatin that revealed how it was so precise in its attachment and potent in its function."

To obtain that overall picture, St. Jude researchers used the analytical technique of X-ray crystallography, with help from nuclear magnetic resonance (NMR) spectroscopy. In this widely used method of determining protein structure, researchers first crystallize a protein to be studied. Then, they direct X-rays through the crystal and deduce the protein structure from the diffraction pattern of those X-rays. To overcome the crystallization bottleneck, a lengthy and unpredictable variable in X-ray crystallography, the investigators used NMR spectroscopy to tailor the perfect enzyme-inhibitor complex.

Tudor Moldoveanu, Ph.D., a postdoctoral fellow in Green's laboratory, performed X-ray structural analysis on such a protein crystal that consisted of a critical part of the calpastatin molecule attached to calpain. The structural picture obtained of the two proteins clutched together clearly revealed why calpastatin so specifically attaches to calpain.

"Calpain has multiple domains, and what we saw was that calpastatin wraps itself around pretty much every domain of calpain," said Moldoveanu, the report's first author. This attachment not only blocks the portion of the enzyme called the active site, where calpain performs its snipping function, but also covers regions away from that site. Such a broad molecular embrace guarantees that calpastatin will potently and rapidly shut down calpain's function, Moldoveanu said. This broad embrace also guarantees that calpastatin will precisely recognize only calpains, rather than mistakenly attach to other similar enzymes in the cell.

Furthermore, the researchers discovered how calpastatin evades being che
'/>"/>

SOURCE St. Jude Children's Research Hospital
Copyright©2008 PR Newswire.
All rights reserved

Page: 1 2 3

Related medicine technology :

1. St. Jude Study Gives New Insights Into How Cells Accessorize Their Proteins
2. Nemours Center for Childhood Cancer Research Team at the Alfred I. duPont Hospital for Children Identifies the Function of a Biomarker Present in Prostate Cancer Cells: New Insight into Therapeutic Benefit for Patients with Advanced Prostate Cancer
3. New Data Offers Insights Into the Potential Role for TYKERB(R) (Lapatinib) in Non-Cytotoxic Chemotherapy-Based Treatment Regimens
4. Apieron, Inc. Begins Customer Shipments of the Insight(TM) eNO System
5. Genmab A/S - Company Announcement: Novel Insights into HuMax-EGFr Mechanisms of Action Published in PNAS
6. Not Only Lipids and Inflammation: Insight Into a New Cause of Heart Attack and Other Vascular Disease
7. PDHI Launches ConXus Rewards, Announces Partnership With Hallmark Insights to Support Incentive-Driven Wellness Programs
8. Chemstar Corporation Announces a New Powerful, One-Step Product That Effectively Controls Listeria, Salmonella, E. coli and Fly Larvae in Floor Drains
9. RAD001 Combined with Sandostatin LAR(R) Depot and as Monotherapy Controls Growth of Rare Pancreatic Neuroendocrine Tumors
10. Enobia Pharma Initiates Clinical Testing of Enzyme Replacement Therapy to Treat Hypophosphatasia
11. Genzyme and Isis Begin Second Phase 3 Trial Of Mipomersen
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/23/2015)... Idaho , Jan. 23, 2015  Now available for ... No. 7,849,611) support that prevents ankle sprains by cushioning ... brace, it fits securely around the outside of any ... mobility & comfort while still offering protection against sprains. ...
(Date:1/23/2015)... LAS VEGAS , Jan. 23, 2015 Cord Blood ... CBAI ) ("CBAI" or the "Company")  filed a Preliminary Proxy ... ( www.sec.gov ) today, and provides an update for shareholders ... authorized shares is requested. Dear Shareholders, ...
(Date:1/23/2015)... (NYSE: MNK ) announced today that its ... The open-ended authorization permits the company to repurchase up ... "Funding additional initiatives and seeking attractive business ... pursue a range of focused growth strategies," said ...
Breaking Medicine Technology:The Armor1 Ankle Roll Guard is the New Breakthrough Solution for Ankle Sprain Prevention! 2Cord Blood America Announces Preliminary Proxy Filing and Details Why a "YES" Vote to Increase Authorized Shares Is Requested 2Cord Blood America Announces Preliminary Proxy Filing and Details Why a "YES" Vote to Increase Authorized Shares Is Requested 3Cord Blood America Announces Preliminary Proxy Filing and Details Why a "YES" Vote to Increase Authorized Shares Is Requested 4Mallinckrodt plc Announces Share Repurchase Program 2Mallinckrodt plc Announces Share Repurchase Program 3Mallinckrodt plc Announces Share Repurchase Program 4
... ORLANDO, Florida, September 14 , ... Surveillance System Will Help Novant,Hospitals Ensure Patient Safety and ... MEDai, Inc., an Elsevier company and leading provider of ... that Novant Health, a not-for-profit integrated group of,hospitals and ...
... , , , , ... Inc. (Nasdaq: AMGN ) today announced the presentation of ... fracture risk reduction among women with postmenopausal osteoporosis. The data were ... Bone Mineral Research (ASBMR). , , "These data ...
Cached Medicine Technology:Novant Health Rolls Out MEDai's Predictive Modeling Solution to Nine Hospitals in Three States 2Novant Health Rolls Out MEDai's Predictive Modeling Solution to Nine Hospitals in Three States 3Novant Health Rolls Out MEDai's Predictive Modeling Solution to Nine Hospitals in Three States 4Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 2Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 3Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 4Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 5Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 6Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 7Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 8Data Show Medication Adherence is an Important Factor in the Treatment of Postmenopausal Osteoporosis 9
(Date:1/22/2015)... Autocarinsurancebest.com has released a new blog post explaining the ... . , Auto insurance quotes help many drivers find ... necessary to call an agent or visit an agency for ... single website: http://autocarinsurancebest.com/ . , Every business who relies ...
(Date:1/22/2015)... A study recently published in ... Medicine & Science in Sports & Exercise shows ... reduce gastrointestinal (GI) problems associated with physical strain ... the double-blind, placebo-controlled study took 10 grams of ...
(Date:1/22/2015)... 2015 Four years since the release of their ... with the marriage of Avasa & Matthew Love – the duo ... scheduled for release through White Swan Records on February 24, 2015. ... a sacred path available to all of us to walk out ...
(Date:1/22/2015)... JJsHouse.com is a famous dress online store for wedding ... its latest collection of wedding dresses , and launches a ... the wedding dress on your big day; the wedding gown ... bride wants to find the most suitable wedding dress. Now, ...
(Date:1/22/2015)... State Forestry Institute of Mecklenburg-Vorpommern in Germany ... from GEOSYSTEMS, a dedicated Hexagon Geospatial and Intergraph partner ... Geospatial’s ERDAS APOLLO for cataloging, managing, and ... clouds and documents. This effort will enable the State ...
Breaking Medicine News(10 mins):Health News:Commercial Auto Insurance Provides Several Important Features! 2Health News:Study Shows Kyowa Hakko’s L-Citrulline May Help Reduce Gastrointestinal Injury During Strenuous Exercise 2Health News:Study Shows Kyowa Hakko’s L-Citrulline May Help Reduce Gastrointestinal Injury During Strenuous Exercise 3Health News:THE ROAD by Avasa & Matthew Love -- A New Album 2Health News:THE ROAD by Avasa & Matthew Love -- A New Album 3Health News:THE ROAD by Avasa & Matthew Love -- A New Album 4Health News:Find The Most Suitable Wedding Dress At JJsHouse.com 2Health News:State Forestry Institute Mecklenburg-Vorpommern, Germany Embraces Hexagon Geospatial Technology 2Health News:State Forestry Institute Mecklenburg-Vorpommern, Germany Embraces Hexagon Geospatial Technology 3
... ... ... ... , March 18 "A good night,s sleep" has long been the intention of millions of people suffering from sleep disorders around the world.  However, this objective is scarcely ...
... Tobacco Taxes, Other Tobacco Prevention Initiatives , ... , , ... ... WASHINGTON , March 18 Kids in New Hampshire will take ...
... of employer-sponsored child care, early education, and work/life solutions, today announced that it has been named to the Center for Companies That Care Honor Roll for the third straight year. ... , ... ... ...
... Higher Tobacco Taxes, Other Tobacco Prevention Initiatives , ... , , ... ... WASHINGTON , March 18 Kids in ...
... MINNEAPOLIS , March 18 ... one that both moms and kids can happily agree on. ... and is free of artificial colors, flavors and sweeteners.  It ... with low fat milk containing both calcium and vitamin D. ...
... Ill. March 18, 2010 Two new studies ... Third Eye Retroscope (TER), a retrograde viewing device, during ... standard colonoscopy detected 13.2 percent more polyps than colonoscopy ... second study examined endoscopist experience using TER and its ...
Cached Medicine News:Health News:Sleep Well, Stay Healthy: Good Quality and Restorative Sleep Are Essential for Better Mental and Physical Health 2Health News:Sleep Well, Stay Healthy: Good Quality and Restorative Sleep Are Essential for Better Mental and Physical Health 3Health News:Sleep Well, Stay Healthy: Good Quality and Restorative Sleep Are Essential for Better Mental and Physical Health 4Health News:New Hampshire Kids 'Kick Butts' on March 24 2Health News:New Hampshire Kids 'Kick Butts' on March 24 3Health News:New Hampshire Kids 'Kick Butts' on March 24 4Health News:Bright Horizons Honored as 'Company that Cares' for Third Straight Year 2Health News:Bright Horizons Honored as 'Company that Cares' for Third Straight Year 3Health News:Montana Kids 'Kick Butts' on March 24 2Health News:Montana Kids 'Kick Butts' on March 24 3Health News:Montana Kids 'Kick Butts' on March 24 4Health News:Montana Kids 'Kick Butts' on March 24 5Health News:New Simply...Go-Gurt Contains No High Fructose Corn Syrup, Artificial Flavors or Colors 2Health News:New Simply...Go-Gurt Contains No High Fructose Corn Syrup, Artificial Flavors or Colors 3Health News:Studies examine Third Eye Retroscope during colonoscopy 2Health News:Studies examine Third Eye Retroscope during colonoscopy 3Health News:Studies examine Third Eye Retroscope during colonoscopy 4Health News:Studies examine Third Eye Retroscope during colonoscopy 5
... The ImmunoWELL VZV IgG ... for the qualitative detection of ... (VZV). Test results are used ... status or infection. Immune status ...
... Test is an enzyme immunoassay (EIA) test ... human thyroglobulin and human thyroid peroxidase (microsome) ... is used as an aid in the ... disorders are characterized by detection of anti-thyroid ...
... ImmunoDOT Autoimmunity Screening Panel 1 is ... screening and detection of autoantibodies against ... SS-A/SS-B, RNP/Sm) in serum, and is ... diagnosis of systemic lupus erythematosus (SLE), ...
... Antibody (IgG) Test is an ... quantitative detection of antibodies to ... is used as an aid ... thrombosis in individuals with systemic ...
Medicine Products: