Navigation Links
New England Biolabs Releases a New Fast, Low-Nanogram Input Library Prep Method for DNA and RNA Sequencing
Date:11/16/2012

IPSWICH, Mass., Nov. 16, 2012 /PRNewswire/ -- New England Biolabs, Inc. (NEB) launched their new NEBNext® "Ultra" kits at the recent American Society for Human Genetics (ASHG) annual meeting. These kits provide streamlined, low-input methods to prepare DNA and RNA libraries for Illumina® next generation sequencing. The supplied protocols and reagents are designed to maximize useful data from a broad range of samples, including those available in limited amounts.

(Logo: http://photos.prnewswire.com/prnh/20090921/NE79207LOGO-b )

The NEBNext Ultra DNA and Ultra RNA Library Kits produce high-yield libraries from 5 ng to 1 ug of input DNA, or as little as 10 ng of input RNA. The input RNA can be total RNA, purified mRNA or rRNA-depleted RNA.

Early access user Cynthia Hendrickson, Ph.D., at the HudsonAlpha Institute for Biotechnology, reports that in initial experiments, NEB's new method "has allowed us to reduce our DNA inputs for exome enrichment from micrograms to a few hundred nanograms, while providing a simpler, faster protocol that minimizes the amount of hands-on time required."

Another early access user, Momchilo Vuyisich, Ph.D., at the Los Alamos National Laboratory, says, "After testing several NGS library prep kits, we have found that the NEBNext Ultra DNA kit for Illumina has the best overall utility for resequencing and assembly of bacterial genomes. The kit offers an unmatched combination of ease-of-use, low cost, robustness and low input DNA requirements. It consistently produces excellent sequencing data."

The new Ultra kits contain novel ligation reagents, as well as NEB's NEBNext NGS-optimized formulation of Q5® High-Fidelity DNA polymerase, which provides ultra high-fidelity amplification and minimized GC bias.

NEB's streamlined protocol for constructing DNA libraries with the Ultra kit requires only 15 minutes of hands-on-time, and is complete in 2.5 hours. The Ultra RNA workflow also incorporates a streamlined protocol; the workflow is complete in 4-5 hours, with only 30 minutes of hands-on time.

For more information on NEB's new Ultra DNA and RNA kits, visit www.NEBNext.com.  

About New England Biolabs   
Established in the mid 1970's, New England Biolabs, Inc. is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, cellular analysis, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and six subsidiaries located in Canada, China, France, Germany, Japan and the UK. For more information about New England Biolabs visit www.neb.com.

ILLUMINA® is a registered trademark of Illumina, Inc.   
NEBNEXT® and Q5® are registered trademarks of New England Biolabs, Inc.


'/>"/>
SOURCE New England Biolabs, Inc.
Copyright©2012 PR Newswire.
All rights reserved

Related medicine technology :

1. 1st SynCardia Total Artificial Heart Implant Performed in New England
2. FAME 2 Published in the New England Journal of Medicine, Volcano participates in the $84M NIH-Sponsored ISCHEMIA Trial
3. Sanofi and Regeneron Announce Publication of Phase 2 Results with LDL Cholesterol-Lowering PCSK9 Antibody in the New England Journal of Medicine
4. ulrich medical USA™ Releases New Expandable Spinal Implant for US Market
5. International Drug & Explosives Detection Company IDenta Corp. Releases Information to OTC markets.
6. ShelfX Releases A Fridge That Knows What You Took
7. Techne Corporation Releases Unaudited Third Quarter and Nine Month Fiscal Year 2012 Results
8. Stereotaxis Releases V-Sono™ Intracardiac Echocardiography Module for the Vdrive™ Remote Navigation System
9. Simbionix Releases Two New Platforms at the Upcoming DDW and AUA Events
10. Medical Alarm Begins Revenue Recognition from Largest Set of Orders -- Releases New Television Commercial
11. MD Bioproducts releases HLA-B27 subtype specific antibody.
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/17/2017)... NEW YORK , Jan. 17, 2017 ... USD 8.02 billion by 2021 from USD 6.35 billion ... major factors driving the growth of this market are ... increasing demand for minimally invasive surgeries. The ... type, procedure, application, and region. On the basis of ...
(Date:1/17/2017)... , Jan. 17, 2017  Jazz Pharmaceuticals plc (Nasdaq: ... patient has been enrolled in a Phase 3 clinical ... best supportive care (BSC) in the prevention of hepatic ... hematopoietic stem cell transplant (HSCT) who are at high ... The defibrotide clinical trial will be conducted across approximately ...
(Date:1/17/2017)... 17, 2017  Market Research Future published a Half Cooked Research ... expected to reach USD 33.6 million during the period 2016 to ... ... Devices Market has been examined as a swiftly growing market and ... demand for endoscopy device in various regions.  The increasing growth in ...
Breaking Medicine Technology:
(Date:1/17/2017)... ... , ... SC&H Group, a leading audit, tax, and consulting firm, announced that ... Services practice . Rossi is the third technology consulting leader to join SC&H Group’s ... and the practice continues to expand.     , Bringing more than 25 years of business ...
(Date:1/17/2017)... ... January 17, 2017 , ... Physicians Education Review®, LCC ... keynote speaker for the 34th Annual Miami Breast Cancer Conference®, on March 9-12, ... president, Phil Talamo, said, “We are delighted to have Sandra Lee join us ...
(Date:1/17/2017)... ... ... make New Year’s resolutions or renew their commitment to better health with the start of ... want to kick off 2017 with better smiles. Dr. Mondavi is offering several promotions for ... , , A new patient package for just $49, which can ...
(Date:1/16/2017)... Boston, MA (PRWEB) , ... January 17, 2017 ... ... winter season, Au Bon Pain, an internationally recognized leader in the fast-casual category, ... new items such as the Good Egg Breakfast Sandwich, Turkey Mash-Up Sandwich, Cranberry ...
(Date:1/16/2017)... ... 2017 , ... Gym Source, America’s leading retailer of premium residential and commercial ... Jersey. , “We are elated to be opening this new showroom,” explains Tom Richard, ... give clients a seamless and motivating shopping experience.” , Every fitness journey is unique, ...
Breaking Medicine News(10 mins):