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Life Technologies Partners with Ridom and Releases SeqSphere+ Software to Speed and Simplify Bacterial Typing
Date:4/18/2013

curacy of influenza monitoring and vaccine development. Members share tools, experience and data using the PathAmp™ FluA Reagents and the Ion PGM™ sequencer.

Bacterial typing using Ion Torrent technology is fast and affordable.  Bacterial typing can be performed in less than a day from library prep to data analysis. The complete solution includes:

  • Ion Xpress® Plus Fragment Library Kit for library construction,
  • Ion Chef® System for high throughput sequencing or Ion OneTouch® 2 System to automate template preparation,
  • Ion PGM® Sequencer and Ion Chips with the 400 base-pair kit for long, accurate reads in as little as three hours,
  • Torrent Suite Software & Ridom SeqSphere+ Software for easy data analysis & assembly.

SeqSphere+ features include:

DNA re-sequencing editor edits and analyzes de novo (.ace) or reference-mapped assemblies (.bam) of NGS data (e.g., MLST, MLST+). Auto-correction of homopolymer related InDel errors.

Analytical tools select data entries from a comparison table for epidemiologic, evolutionary or functional analysis. Cluster and visualize data entries by using minimum spanning or UPGMA/Neighbor Joining trees.

Database to store, search, retrieve, export and create reports from your epidemiologic and DNA sequence data stored in an integrated database. Search new sequence entries against stored data.

Bacteria typing is automatically performed with user defined quality parameters (e.g., coverage, stop codons, frame shift detection, etc.) using public or self-defined query libraries and task templates.

Products referenced above are For Research Use only, not for use in diagnostic procedures.

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