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Ion Torrent Cancer Panel Finds Mutations in One Day
Date:10/12/2011

iversity Nijmegen Medical Centre in The Netherlands. "The amount of required starting material is incredibly small, considering how many mutations can be tested.  In our first experiment all KRAS mutations previously identified with TaqMan® Mutation Detection assays and Sanger sequencing were confirmed."

"Next generation sequencing will change the clinical management of the cancer patient," said Prof Pierre Laurent-Puig, who runs the Clinical Oncogenetic Unit in the Department of Genetics at the Paris - Descartes University Medical School. "The Ion AmpliSeq™ Cancer Panel could help us fill some existing gaps between classical mutation detection and exome sequencing. Our testing of this new technology has allowed detection of KRAS and EGFR alterations in colon and lung cancer research samples preserved in paraffin embedded tissue."

"This next generation sequencing technology permits deep sequencing of a panel of mutations at the same time. It can detect mutations at frequencies as low as 5 percent with short turn around time and low cost," said Marilyn M. Li, M.D., director of the Cancer Genetics Laboratory at Baylor College of Medicine. "This could be an ideal solution for us and we see a great potential for diagnostic applications."

By merging multiplex PCR with massively parallel Ion semiconductor sequencing, the Ion AmpliSeq™ Cancer Panel generates data needed to advance oncology research and improve life.

For Research Use Only.  Not intended for any animal or human therapeutic or diagnostic use.

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