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Global Cell/Tissue Culture Supplies Industry
Date:3/16/2011

elated Technologies 19

Somatic Cell Nuclear Transfer (SCNT) 19

Tissue Engineering 19

End Use Industries 19

5. CELL/TISSUE CULTURE: PHYSIOLOGICAL OVERVIEW 20

Introduction 20

History 20

Plant Cell and Tissue Culture (PCTC) 24

Cell/Tissue Culture Media, Sera and Reagents 27

Classical Media 27

Specialty Media 28

Serum 28

Fetal Bovine Serum 28

Serum-Free Media 28

Cell Culture Reagents 29

Role of Tissue Culture in the Growth of Cell Types 29

Equipment 29

Storage and Preservation 29

Maintenance 30

Growth Pattern 30

Harvesting 30

Media and Growth Requirements 31

Physiological Parameters 31

Medium Requirements (Often Empirical) 31

Feeding 31

Safety Precautions 31

Methods of Tissue Culture 32

Preparation of Media 32

Growth and Morphology 32

Sub Culturing Adherent Cells 32

Thawing Frozen Cells 32

Freezing Cells 32

Cell Culture Contamination - A Looming Problem 33

Classification 33

Major Cell Culture Contaminants 33

Chemical Contamination 33

Types and Sources of Potential Chemical Contaminants 33

Varied Sources of Chemical Contamination 34

Media and Components 34

Sera 34

Water 35

Storage Vessels 35

Fluorescent Lights 35

Endotoxins 35

Incubators 36

Biological Contamination 36

Varied Sources of Biological Contamination 37

Mycoplasmas 37

Airborne Particles and Aerosols 37

Cross Contamination by Other Cell Cultures 38

Consequences/Problems of Contamination 38

Controlling Cell Culture Contamination 38

Measures Aimed at Reducing Contamination Problems 39

6. REGULATORY ENVIRONMENT 40

7. PRODUCT INNOVATIONS/INTRODUCTIONS 41

StemCells Introduces GS1-RT
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1% tryptone, 0.5% yeast extract, 1.0% Sodium Chloride. 1.5% agararose...
Luria bertani broth: 1.0% Tryptone, 0.5% Yeast Extract, 1.0% Sodium Chloride....
... flowing powder. pH: pH(4.7%, H 2 O): ... Yeast Extract: 47% Potassium Phosphate Dibasic: 25% Potassium ... 47 gm powder per liter of purified water. ... and recombinant proteins than LB media by allowing ...
1.2% Tryptone, 2.4% Yeast Extract, 0.4% Glycerol, with 100ml of 10x Phosphate buffer supplement (0190-01)....
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