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Getting More From Whole-Transcript Microarrays
Date:5/22/2009

on about adjacent poorly fitting probes to calculate differential expression in the Gene arrays and showed that it provides comparable results to the Exon array analysis. The approach can only be used in well-annotated genes and can detect differential splicing involving multiple exons. However, they suggested that it should work particularly well for genes containing few exons, since for these genes the coverage of probes in the Gene platform can be greater than the Exon platform.

Dr Robinson added that, "To the best of our knowledge, this is the first statistical method that interrogates differential splicing using the Gene 1.0 ST platform. We have used this method for uncovering differential splicing in human tissues, where typically a small number of tissues exhibit a distinct pattern. However, we believe it could be useful in a variety of experimental settings." FIRMAGene can be applied to human, mouse and rat samples, for which the latest Affymetrix Gene 1.0 ST platforms are available, or any other whole-transcript microarray design.

The Walter and Eliza Hall Institute is one of the world's leading biomedical research centres. Established in 1915 and affiliated with The University of Melbourne and The Royal Melbourne Hospital, the Institute's mission is 'Mastery of Disease through Discovery'. Its world-renowned research teams have been responsible for breakthrough science and pioneering therapies that have changed the face of disease and human health worldwide. Today they are focused on four big global medical challenges: cancer, immunity, autoimmunity and infectious diseases. The Walter and Eliza Hall Institute is located at Parkville, Victoria where it will soon be housed in a state-of-the-art new building.

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