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DSM Expands its Innovative Biomanufacturing Technology Portfolio With the Introduction of the New Kremer Method™
Date:6/27/2011

lity."

Rolf Douwenga, Vice President of Global R&D for DSM Biologics explained: "The Kremer Method™ is a valuable addition to the platform of DSM's technologies addressing the full range of mammalian cell culture systems for protein and monoclonal antibody production. The method uses in-line dilution to streamline the purification and polishing of mAbs into one single continuous step in a flow-through mode. This eliminates the need for storage and reduces the amount of material usage such as buffers and resins. The Kremer Method™ reduces processing and preparation times, labor requirements and the cost of goods and presents an ideal solution to achieving cost effective processing."

After the capture step in conventional downstream processes, further purification and polishing of proteins are traditionally carried out as two separate batch unit operations. With the Kremer Method™ these steps are streamlined to work as one single unit operation.  Using this method; Host Cell Proteins (HCP's) were removed to a level below the limits of detection; complete aggregate removal was observed comparable to current state of the art processes; and recoveries of 90% were achieved. Several patent applications have already been filed.

XD® is a registered trademark of DSM

Rhobust® is a registered trademark of DSM

DSM – Bright Science. Brighter Living.™ DSM Pharmaceutical Products is a Business Group of Royal DSM N.V., a global science-based company active in health, nutrition and materials. By connecting its unique competences in Life Sciences and Materials Sciences DSM is driving economic prosperity, environmental progress and social advances to create sustainable value for all stakeholders. DSM delivers innovative solutions that nourish, protect and improve performance in global markets such as food and dietary supplements, personal care, feed, pharmaceuticals, medical devices, auto
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