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Cardium Presents Second Quarter 2012 Financial Results And Reports On Recent Developments
Date:8/14/2012

man Gene Therapy Methods in an article entitled "Ischemia-Reperfusion Increases Transfection Efficiency of Intracoronary Adenovirus type 5 in Pig Heart in Situ," which is available online at http://online.liebertpub.com/doi/full/10.1089/hgtb.2012.048.

The published findings demonstrate that Cardium's innovative technique employing transient cardiac ischemia can be used to dramatically enhance gene delivery and transfection efficiency after one-time intracoronary administration of adenovector in mammalian hearts. Two consecutive but brief periods of coronary artery occlusion combined with co-administration of nitroglycerin increased both adenovector presence (measured by PCR) and transgene expression (assessed by luciferase activity) by over two orders of magnitude (>100 fold) in the heart, as compared to prior intracoronary artery delivery methods.  The research findings have been incorporated into the treatment protocols of the Company's recently-initiated Generx® [Ad5FGF-4] ASPIRE study.  The Russian-based ASPIRE Phase 3 registration study of patients with chronic myocardial ischemia and advanced angina pectoris uses transient ischemia techniques during non-surgical percutaneous catheterization with a standard angioplasty catheter together with the intracoronary infusion of nitroglycerin with the Generx product candidate.

As recently reported in the New York Times, the European Medicines Agency (EMA) has recommended approval of uniQure's Glybera gene therapy for the treatment of lipoprotein lipase deficiency.  If the recommendation is followed, this will represent the first gene therapy regulatory approval in the Western world and a significant step forward in the field of gene-based therapeutics.About Generx and the ASPIRE StudyGenerx (Ad5FGF-4) is a disease-modifying regenerative medicine biologic that is being developed to
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