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CRi Oosight Imaging System a Key to Breakthrough Gene Replacement Method With Potential to Prevent Inherited Mitochondrial Diseases
Date:8/27/2009

usly generating useful quantitative data of their structural composition. Two of the four offspring, Spindler and Spindy, were named after the spindle, which is what the Oosight system is used to visualize.

"This study underscores the potential of the Oosight system to advance reproductive medicine and highlights the enabling capabilities or our polarized light technology," said George Abe, president and CEO of CRi.

"With this advance, the Oosight imaging system, which is already widely used in fertility clinics, has offered new insights and possibilities into reproductive health and medicine," said Gary Borisy, director and CEO of the Marine Biological Laboratory (MBL) in Woods Hole, MA. The Oosight system is based on imaging technology originally developed by MBL scientists Rudolf Oldenbourg and Michael Shribek, working in collaboration with David Keefe, M.D., of the University of South Florida College of Medicine.

In in vitro fertilization (IVF) the Oosight system is used as an aid to intracytosplasmic sperm injection (ICSI). The system not only provides assurance that the genetic material is not damaged by the injection needle, but it can also be used as a measurement tool to assess egg viability in both fresh and frozen eggs. Data show that an egg with a weak or malformed spindle and inner layer zona as measured with the system is much less likely to result in pregnancy.

Other scientists have welcomed news of the advance. Mitochondria-expert Douglas Wallace of the University of California, Irvine, said "results were exciting" and the technique is "potentially very interesting." Although he did caution that "there are safety issues that are going to need to be addressed before one could think about it in humans."

The Nature article reported that 15 embryos were transplanted into nine surrogate mothers; three became pregnant, one with twins, and four offspring
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SOURCE Cambridge Research & Instrumentation, Inc.
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