Navigation Links
CRi Oosight Imaging System a Key to Breakthrough Gene Replacement Method With Potential to Prevent Inherited Mitochondrial Diseases
Date:8/27/2009

WOBURN, Mass., Aug. 27 /PRNewswire/ -- U.S. researchers using CRi's Oosight(TM) imaging system have developed a gene transfer technique that has potential to prevent inherited diseases passed on from mothers to their children through mutated DNA in cell mitochondria. The research, which demonstrated the technique in rhesus monkeys, appears in the Aug. 26 issue of the journal Nature.

The group, headed by Dr. Shoukhrat Mitalipov of the Oregon National Primate Research Center and the Oregon Stem Cell Center, extracted the nuclear DNA from the mother's egg, guided by the Oosight system, and transplanted it into another egg that had the nucleus removed. The technique allowed the mother to pass along her nuclear genetic material to her offspring without her mitochondrial DNA. The eggs were fertilized and transplanted into surrogate mothers, resulting in the birth of four apparently healthy monkeys. Defects in DNA of mitochondria, the cell's "power plants," are associated with a wide range of human diseases.

The Oosight system solved a key problem in avoiding damage to the nuclear DNA during the transfer procedure by providing a non-invasive imaging technique for visualizing the genetic material. Traditional visualization methods employ a stain or involve exposure to ultraviolet light, either of which can damage DNA. The Oregon team had used the Oosight system in previous research, published in Nature in 2007, that provided a foundation for the current study. In that research, they cloned rhesus monkey embryos and used them to create embryonic stem cells.

The Oosight system uses polarized light to generate high-contrast, real-time images of biological features such as the spindle apparatus housing the chromosomes and other filamentous structures within the egg, such as the multi-layer zona pellucida, without the addition of toxic stains or labels, while simultaneously generating useful quantitative data of their structural composition. Two of the four offspring, Spindler and Spindy, were named after the spindle, which is what the Oosight system is used to visualize.

"This study underscores the potential of the Oosight system to advance reproductive medicine and highlights the enabling capabilities or our polarized light technology," said George Abe, president and CEO of CRi.

"With this advance, the Oosight imaging system, which is already widely used in fertility clinics, has offered new insights and possibilities into reproductive health and medicine," said Gary Borisy, director and CEO of the Marine Biological Laboratory (MBL) in Woods Hole, MA. The Oosight system is based on imaging technology originally developed by MBL scientists Rudolf Oldenbourg and Michael Shribek, working in collaboration with David Keefe, M.D., of the University of South Florida College of Medicine.

In in vitro fertilization (IVF) the Oosight system is used as an aid to intracytosplasmic sperm injection (ICSI). The system not only provides assurance that the genetic material is not damaged by the injection needle, but it can also be used as a measurement tool to assess egg viability in both fresh and frozen eggs. Data show that an egg with a weak or malformed spindle and inner layer zona as measured with the system is much less likely to result in pregnancy.

Other scientists have welcomed news of the advance. Mitochondria-expert Douglas Wallace of the University of California, Irvine, said "results were exciting" and the technique is "potentially very interesting." Although he did caution that "there are safety issues that are going to need to be addressed before one could think about it in humans."

The Nature article reported that 15 embryos were transplanted into nine surrogate mothers; three became pregnant, one with twins, and four offspring were born (only three of these offspring have been reported in the Nature paper) The success rate is similar to that of conventional in vitro fertilization.

A sample movie of the enucleation process that Dr. Mitalipov used is available athttp://www.cri-inc.com/multimedia/Oosight_SCNT_Enucleation_Rhesus_Monkey.avi, (movie courtesy Dr. Mitalipov, OHSU).

Contact CRi at oosight@cri-inc.com for more details.

Cambridge Research & Instrumentation, Inc. (CRi) is a leader in biomedical imaging, and is dedicated to providing comprehensive solutions that analyze disease-specific information from biological and clinical samples in the combined physiological, morphological, and biochemical context of intact tissues and organisms for a variety of applications. With over 80 patents pending and issued, CRi's award-winning innovations are being utilized around the world to enable our customers to perform leading research and provide better healthcare.

    For more information contact:
    Cathy Boutin
    Marketing Manager
    Cambridge Research & Instrumentation, Inc. (CRi)
    Tel. 781-935-9099 x180
    Email: cboutin@cri-inc.com


'/>"/>
SOURCE Cambridge Research & Instrumentation, Inc.
Copyright©2009 PR Newswire.
All rights reserved

Related medicine technology :

1. CRi Oosight(TM) Instrument Crucial in IVF Breakthrough Demonstrating a Correlation Between Non-Invasive Egg Metrics and Pregnancy
2. ProUroCare Medical Completes Clinical Trial at Second Study Site Using Digital Prostate Imaging Technology
3. VisEn Molecular Imaging Technology Enables Key Insights Into Newly Discovered Biologic Pathway Published in SCIENCE
4. Elbit Imaging Ltd. Announces - Swiss Team Uses InSightecs ExAblate(R) 4000 Brain System to Treat Patients With Functional Brain Disorders
5. Perceptive Informatics Enhances Investigator Application of New RECIST Version in Imaging-Based Oncology Trials
6. Perceptive Informatics Introduces Medical Imaging Methodology to Accelerate Development Decisions About Alzheimers Treatments
7. New Imaging Analysis Predicts Brain Tumor Survival
8. Varian Medical Systems to Exhibit Cancer Treatment and X-ray Imaging Technologies at China Med Exhibition
9. VirtualScopics Quantitative Imaging Provides Primary Endpoint in Cartilage Repair Pivotal Clinical Trial
10. New Breast Imaging Technology Targets Hard-to-Detect Cancers
11. FluoroPharma, Inc. to Report Preclinical Data on Alzheimers Disease and Prostate Cancer PET Imaging Agents
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/5/2017)... Ill. , Oct. 5, 2017  In ... Association of Oral and Maxillofacial Surgeons (AAOMS) released ... opioids – to be used as a first-line ... pain. Recognizing ... the AAOMS White Paper "Opioid Prescribing: Acute and ...
(Date:10/4/2017)... Oct. 4, 2017 OBP Medical ... illuminating medical devices, today announced regulatory approval from ... Agency (or Agência Nacional de Vigilância Sanitária (ANVISA)) ... cordless surgical retractor with integrated LED light source ... illumination and exposure of a tissue pocket or ...
(Date:10/2/2017)... Oct. 2, 2017  AllianceRx Walgreens Prime, the combined ... Walgreens and pharmacy benefit manager Prime Therapeutics LLC (Prime), ... which included the unveiling of new signage at its ... well as at a few other company-owned facilities across ... to patients, some of whom will begin to see ...
Breaking Medicine Technology:
(Date:10/12/2017)... ... , ... Planet Fitness, one of the largest and fastest growing franchisors and ... flagship location in Covington, LA at 401 N. U.S. Highway 190, in January of ... Depot in the Holiday Square shopping center. Its location allows it to serve both ...
(Date:10/12/2017)... ... 12, 2017 , ... IsoComforter, Inc. ( https://isocomforter.com ), one of ... innovative new design of the shoulder pad. The shoulder pad provides optimal support ... your pain while using cold therapy. By utilizing ice and water that is circulated ...
(Date:10/12/2017)... ... October 12, 2017 , ... Asante, a nationally recognized health ... expanded their existing home health joint venture through an agreement, effective October 1, ... joint venture home health company with Asante, delivering clinically integrated care, for the ...
(Date:10/12/2017)... ... 2017 , ... Dr. Parsa Mohebi, the Los Angeles based ... the newly revamped Cosmetic Town journal section, featuring articles written by ... as Follicular Unit Extraction (FUE). , Dr. Mohebi says “I enjoy ...
(Date:10/12/2017)... ... ... Leading pediatric oncology experts at Children’s National Health System will ... the International Society of Paediatric Oncology (SIOP) Oct. 12-15. Chaired by Jeffrey ... Disorders at Children’s National, and Stephen P. Hunger, M.D., Chief of the Division ...
Breaking Medicine News(10 mins):