Navigation Links
BGI and GT Life Sciences, Inc. Report Genome Sequence of CHO-K1 and Provide New Insights into Optimization of Biopharmaceutical Protein Production
Date:8/1/2011

added.

GT Life Sciences' established business platform for metabolic modeling and engineering of mammalian cells has already proven effective in optimizing CHO cell media and developing novel selectable markers. With the advanced genome sequencing capability of BGI, the CHO-K1 ancestral cell line was sequenced by de novo sequencing and then assembled by BGI's Short Oligonucleotide Analysis Package, SOAPdenovo, resulting in the assembly of 2.45 Gb of the genomic sequence. This information was combined with transcriptome sequence data and led to the generation of 24,383 predicted genes.

"After working with cell culture for over 20 years, this is a dream come true," said Bernhard Palsson, Ph.D., co-founder of GT Life Sciences. "We can now hope to develop metabolic and cell line engineering procedures that approach those currently available for E. coli and yeast. This development signifies the start of genome-scale science for CHO."

"The CHO cell line genome revealed the secret of its protein synthesis, modification and viral resistance, which are very unique among other cell lines and make it the optimal protein production tool," said Xu. Because of the fact that differential glycosylation can substantially affect functional activity and immune responses, researchers carried out a genome-scale assessment of CHO-K1 genes involved in protein glycosylation pathways. They identified homologs to 99% of the human glycosylation-associated genes in the CHO-K1 genome, with 53% of them expressed. The high coverage of homologs provides a unique opportunity for glycoform manipulation in CHO cells. According to the report, the genome of CHO-K1 also provided insight into viral susceptibility genes and found that key genes associated with viral entry are not expressed in CHO-K1.

"Coupling the CHO sequence with a complete genome-scale model of CHO metabolism will prove highly productive in our ability to deliver next-generation process techn
'/>"/>

SOURCE GT Life Sciences, Inc.
Copyright©2010 PR Newswire.
All rights reserved

Page: 1 2 3 4

Related medicine technology :

1. Luminex Corporation Completes Acquisition of EraGen Biosciences, Inc.
2. Luminex Corporation Announces Agreement to Acquire EraGen Biosciences, Inc.
3. Regado Biosciences, Inc. Presents Results of the RADAR Phase 2b Clinical Trial of its REG1 Anticoagulation System
4. Regado Biosciences, Inc. Announces Presentation of the Results of the RADAR Phase 2b Clinical Trial of Its Anticoagulant System REG1 at the i2 Summit During the American College of Cardiology 2011 60th Annual Scientific Session & Expo in New Orleans
5. Regado Biosciences, Inc. Introduces REG3, a Novel Aptamer-Based Inhibitor of Glycoprotein VI and its Matched Active Control Agent, at the American Heart Association (AHA) Scientific Sessions 2010 Meeting
6. Regado Biosciences, Inc. Presents Pharmacokinetic and Pharmacodynamic (PK/PD) Substudy Results From its Phase 2b RADAR Trial at the American Heart Association (AHA) Scientific Sessions 2010 Meeting
7. Regado Biosciences, Inc. Presents Two Abstracts on the Subcutaneous Depot Formulation of Pegnivacogin, a Nuclease-Stabilized RNA Aptamer, Direct Factor IXa Inhibitor and Part of the REG2 Anticoagulant System, at the American Heart Association (AHA)
8. Coronado Biosciences, Inc. Announces Completion of Financings, Management Changes and Development Update
9. Regado Biosciences, Inc. Presents the First-In-Human Clinical Experience with REG2 at the American College of Chest Physicians (ACCP) Coagulation and Heart Disease Sessions in Vancouver, BC, Canada on November 2, 2010
10. Signum Biosciences, Inc. Announces Commercial Launch of Arazine™
11. Ampio Pharmaceuticals to Acquire DMI Biosciences, Inc.
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/21/2015)... Inc. (NYSE: CRY ), a leading medical device ... surgery, announced today that its Board of Directors has ... 2015 of $0.03 per share of common stock outstanding.  ... be paid on June 19, 2015 to all common ... ex-dividend date for the quarterly dividend is June 10, ...
(Date:5/21/2015)... -- The Pharmaceutical Care Management Association (PCMA) issued ... offsets included in the Energy & Commerce Committee,s ... 21st Century Cures initiative can only be advanced by ... Medicare – then its costs outweigh any potential ... "Leveraging payments to Part D plans to ...
(Date:5/21/2015)... 2015  Heidrick & Struggles (Nasdaq: HSII ), ... leadership consulting and culture shaping worldwide, ... practices announcing the addition of Tim Dietlin ... Dietlin has extensive life sciences and ... R&D, Medical and Commercial functions to increase productivity, drive ...
Breaking Medicine Technology:CryoLife Announces Quarterly Cash Dividend for the Second Quarter 2015 2Heidrick Increases Healthcare and Life Sciences Expertise 2
... 1, 2012  ProUroCare Medical Inc. (OCTBB: PUMD), provider ... has received clearance from the U.S. Food & ... imaging (PMI) system.  The approval paves the way ... visual documentations as an aid in detecting prostate ...
... 1, 2012 MAP Pharmaceuticals, Inc. (Nasdaq: MAPP ) ... conferences in May 2012: Deutsche Bank 37th Annual Healthcare ... Bank of America Merrill Lynch 2012 Healthcare Conference Tuesday, May ... live webcast of each event will be available on the Investor ...
Cached Medicine Technology:ProUroCare Medical Receives FDA Clearance for ProUroScan Elasticity Imaging System 2ProUroCare Medical Receives FDA Clearance for ProUroScan Elasticity Imaging System 3
(Date:5/23/2015)... AZ (PRWEB) May 23, 2015 Manufacturers are ... Seasonal Performance Factor (HSPF) of 8.2. Experts in the field ... ultimately benefit consumers. , The HSPF score is what miles ... the more efficient the unit. Although the minimum HSPF score ... water heaters with an HSPF score north of 12. Indeed, ...
(Date:5/23/2015)... 23, 2015 Add High-Quality prismatic refractions into ... Studios. , It's as simple as dragging and dropping footage ... tool for full effect. , Including over 80 stunning ... 5k sensors making this the ultimate bundle for adding prismatic ... in this package are movie files designed for Final Cut ...
(Date:5/22/2015)... Pioneer Millworks, the largest reclaimed wood company in ... reclaimed wood, introducing their latest reclaimed wood products at ... offerings, Vat 35, Reclaimed Teak , and Prairie ... largest design event. , “Designers, architects, builders, and homeowners ... see and feel the variety of wood surfaces in ...
(Date:5/22/2015)... East Liverpool, OH (PRWEB) May 22, 2015 ... today announced that Kyle Johnson, the health system’s Chief ... pursue other interests. Johnson has served as the company ... announced Wesley West will be joining the health system ... operations on an interim basis, while the Board of ...
(Date:5/22/2015)... Omar Ahmad Barrada, a junior at Milton Hershey ... Congress of Future Medical Leaders in Boston, Mass. The ... highest achieving high school students.” , “I’m excited to ... and learn better study habits,” said Omar. “The event ... can ask the surgeons questions.” , The goal is ...
Breaking Medicine News(10 mins):Health News:Parker & Sons Welcomes the Department of Energy’s Water Heater Regulation 2Health News:Developers at Pixel Film Studios Release ProFlare 5K Prism for Final Cut Pro X. 2Health News:Pioneer Millworks to Reveal New Design Trends with Reclaimed Wood at Dwell on Design in LA 2Health News:Pioneer Millworks to Reveal New Design Trends with Reclaimed Wood at Dwell on Design in LA 3Health News:River Valley Health Partners Announces CFO Transition, Appointment of Interim CFO 2Health News:Milton Hershey School Student Chosen for Congress of Future Medical Leaders 2
... are more effective at delivering protection against influenza virus ... detailed analysis of the early immune responses by the ... the skin is such fertile ground for vaccination with ... was published in the January/February issue of the online ...
... "report cards" to grade health care providers, greater attention to ... such efforts, according to a new RAND Corporation study. ... care providers can do much to improve the nation,s health ... author and researcher at RAND, a nonprofit research organization. "But ...
... PHILADELPHIA - Researchers from the Perelman School of Medicine ... Cancer Research a universal approach to personalized cancer therapy ... a system for making an adaptable, engineered T-cell to ... which abnormal proteins, called antigens, are expressed by individual ...
... -- Preteen and teenage girls whose diets are rich in ... particularly if they are involved in high-impact activities, according to ... followed over 6,700 girls, ranging in age from 9 to ... of seven years. The study, published in the March 5 ...
... and high-school students who bully their classmates are more likely ... marijuana, a new study found. Researchers found that bullies ... were more likely to use substances than were victims ... behavior may be related to another," said Kisha Radliff, lead ...
... -- Children with asthma have a higher risk for developing ... the herpes zoster virus, new research reveals. The authors ... with the herpes zoster virus every year. However, typically it,s ... of 60 or people with weakened immune systems. Researchers ...
Cached Medicine News:Health News:Microneedle vaccine patch boosts flu protection through robust skin cell immune response 2Health News:Report suggests ways to improve health-care provider 'report cards' 2Health News:New universal platform for cancer immunotherapy developed by Penn-led team 2Health News:New universal platform for cancer immunotherapy developed by Penn-led team 3Health News:Vitamin D May Cut Stress Fracture Risk in Girls 2Health News:School bullies more likely to be substance users, study finds 2Health News:School bullies more likely to be substance users, study finds 3Health News:Children With Asthma at Higher Risk for Shingles: Study 2
... The SYNCHRON CX4 Delta Specialty ... random access analyzer that can consolidate ... specialty testing, such as thyroid, HbA1c ... The CX4 Delta offers a level ...
The Rheumatoid Factor (RF) assay is used for the quantitative analysis of rheumatoid factor in human serum....
Rheumatoid Factor , R1: 4 X 20 ml buffer, R2: 2 X 8 ml antibody, Test / Kit: 320 (H717)...
EXTEND is an absorbable synthetic punctal implant that provides occlusion up to three months. It is packaged 20 implants per box in sizes of .0.3mm, 0.4mm, 0.5mm. A variety pack in also available w...
Medicine Products: