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Vitamin D 25-Hydroxy ELISA 026-AC-57F1 Bone and Mineral Metabolism

ProductsVitamin D 25-Hydroxy ELISA 026-AC-57F1 Bone and Mineral Metabolism
Company ALPCO Diagnostics
Item Vitamin D 25-Hydroxy ELISA 026-AC-57F1 Bone and Mineral Metabolism
Features 
Description The OCTEIA 25-Hydroxy Vitamin D kit is an enzymeimmunoassay for the quantitation of 25-OH D and other hydroxylated metabolites in serum or plasma. Calibrators, controls and samples are diluted with biotin labelled 25-OH D. The diluted samples are incubated in microtitre wells which are coated with a highly specific sheep 25-OH D antibody for 2 hours at room temperature before aspiration and washing. Enzyme (horseradish peroxidase) labelled avidin, is added and binds selectively to complexed biotin and, following a further wash step, colour is developed using a chromogenic substrate (TMB). The absorbance of the stopped reaction mixtures are read in a microtitre plate reader, colour intensity developed being inversely proportional to the concentration of 25-OH D.
Info ALPCO DiagnosticsCall ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) USACanada1-866-466-7731

Customer Service: (800) 592-5726
Web site: http://www.alpco.com

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