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VerifyNow IIb/IIIa Assay

ProductsVerifyNow IIb/IIIa Assay
Company Accumetrics
Item VerifyNow IIb/IIIa Assay
Features Each single-use disposable assay device, such as the VerifyNow IIb/IIIa or VerifyNow Aspirin Assay, contains all needed reagents to provide analytical results for a specific assay. Assay devices are individually pouched and may be used until the expiration date printed on each kit box and foil pouch.

The assay device consists of the following components:

  • Finger Grip: Each assay device has a handle with which the user can insert or remove the assay device from the assay device port.
  • Staging Well: The blood sample is held in this area for temperature stabilization prior to analysis.
  • Assay Device Spot Code: Each assay device is labeled with a unique spot code. Upon insertion of the assay device into the assay device port, the instrument automatically scans the spot code and determines which assay is being performed, records the lot number, and verifies that the assay device has not expired.
  • Sample Well: The sample well is the area of the assay device where the blood collection tube is inserted. The well contains a Blood Collection Tube Spike for sampling directly from a closed blood collection tube. A protective sheath is removed from the assay device spike before use.
  • Humidity Indicator: Each assay device has a humidity sensor to determine whether the assay device has been exposed to excess humidity.
  • Mixing Chamber/Detection Wells: The wells contain all necessary reagents for the specific assays.
Description The VerifyNow IIb/IIIa Assay is an automated, turbidimetric, whole- blood assay designed to assess platelet function based upon the ability of activated platelets to bind to fibrinogen coated beads. The detection well of the VerifyNow IIb/IIIa assay device contains all of the necessary reagents to perform this analysis. Within the well is an activator that induces the platelet to change the conformation of the GP IIb/IIIa receptor to a form that binds fibrinogen (Iso-TRAP). Additionally, the detection well also contains fibrinogen-coated microbeads that bind to activated GP IIb/IIIa receptors. The GP IIb/IIIa receptors on activated platelets will bind to the fibrinogen-coated microbeads and cross link to other microbeads resulting in a clearing of the beads and platelets within the detection well. The instrument uses light transmittance to measure the rate at which this clearing occurs. If the GP IIb/IIIa receptors on the platelet are inhibited by abciximab or eptifibatide, there will be minimal binding of the microbeads with activated platelets, since the GP IIb/IIIa receptor sites are blocked by the drug and cannot bind to the fibrinogen coated beads. In this instance, there will be minimal clearing of the sample, and very little change in the amount of light that is transmitted through the sample.
Info Accumetrics
Customer Service: (800) 643-1640
Web site: http://www.accumetrics.com

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