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Ultrasensitive C-Peptide EIA Diabetes and Obesity Markers 008-10-1141-01

ProductsUltrasensitive C-Peptide EIA Diabetes and Obesity Markers 008-10-1141-01
Company ALPCO Diagnostics
Item Ultrasensitive C-Peptide EIA Diabetes and Obesity Markers 008-10-1141-01
Features 
Description Qualitative and quantitative evaluation of pancreatic -cell function is not only of use in the pre- and post-diagnostic study of the natural history of diabetes mellitus, but is also relevant in clinical practice as a guide to the correct choice of treatment. Peripheral insulin levels cannot be used to assess -cell function because of the large and variable uptake from the portal circulation into the liver, and because insulin assays cannot distinguish endogenous from exogenous insulin1,3. Human C-peptide is a 31 amino acid chain with a molecular mass of approximately 3020 daltons. Within the pancreatic -cell, proinsulin is cleaved into one molecule of C-peptide and one molecule of insulin. C-peptide is subsequently released into the circulation at concentrations equimolar to those of insulin. In contrast to insulin, C-peptide is only minimally extracted by the liver. Peripheral C-peptide concentrations therefore reflect the secretion of -cells more accurately than insulin2,4 The ALPCO C-peptide ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. During incubation, C-peptide in the sample reacts with peroxidase-conjugated anti-C-peptide antibodies and anti-C-peptide antibodies bound to microplate wells. A simple washing step removes unbound enzyme labeled antibody. The bound conjugate is detected by reaction with 3,3',5,5'-tetramethylbenzidine (TMB). The reaction is stopped by adding acid. The colorimetric endpoint is read spectrophotometrically.
Info ALPCO DiagnosticsCall ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) USACanada1-866-466-7731

Customer Service: (800) 592-5726
Web site: http://www.alpco.com

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... are available as both regular and filter ... gamma-rays for more efficient sterilization and are ... the date of irradiation. Sterilized Diamond Tips ... autoclave. SterilPack Diamond Tips are wrapped in ...
300 L, Bulk Recommended for 250 and 300 L pipettes....
Ambion's barrier tips prevent cross-contamination of samples by means of an aerosol filter. They are RNase- and DNase-free and are ideal for handling RNA, PCR applications, and for working with radio...
Designed to handle larger volumes, this tip has all the features of the ART XLP but has been modified to draw a full 200 ul....
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