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Total T4 EIA Test

ProductsTotal T4 EIA Test
Company Diagnostic Systems Laboratories, Inc.
Item Total T4 EIA Test
Description Thyroxine (T4) is the primary active hormone synthesized within the follicular cells of the thyroid gland [1]. T4 is synthesized via a series of steps involving: (i) iodide trapping by the follicular cells, (ii) iodination of tyrosine residues in the thyroglobulin substrate leading to formation of 3-monoiodotyrosine and 3,5-diiodotyrosine (MIT and DIT), and (iii) coupling of MIT and DIT to form either T4 or T3 (triiodothyronine). The production of T4 is approximately 3 times greater than that of T3. In plasma, ~70% of T4 is bound to thyroxine-binding globulin (TBG), 15-25% to transthyretin, 5-15% to albumin and a small percentage is bound to erythrocytes [1,2]. Less than 0.1% of the total T4 circulates in a free or unbound form. T4 binds to specific cell receptors and has diverse cellular and somatic effects. Measurement of total serum T4 levels is a standard and well-validated test of thyroid gland function [3].

  1. Physiology of thyroid hormones. IN: Division of Drugs and Toxicology, American Medical Association: Drug Evaluations Annual 1995. Amer Med Assn, Chicago, 1995, ch 47, pp 1039-1040.
  2. Bartalena L. Endocrin Rev 11:47-64, 1990.
  3. Gruhn JG, et al. Arch Pathol Lab Med 111:84-100, 1987.
Info Diagnostic Systems Laboratories, Inc.
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