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Total PSA ELISA Test

ProductsTotal PSA ELISA Test
Company Diagnostic Systems Laboratories, Inc.
Item Total PSA ELISA Test
  • Requires only 25 L serum sample to test
  • No sample pretreatment required
  • Incubation is 1 hr + 30 mins + 10 mins on shaker at RT
Description Prostate-Specific Antigen (PSA) derives its name from its first known site of origin, the prostate gland. The majority of PSA circulates bound to anti-chymotrypsin (PSA-ACT) and other compounds. Serum concentrations of PSA are elevated in patients with prostate cancer, benign prostatic hypertrophy (BPH) and prostatitis [1-3]. In addition, PSA serum levels appear to correlate with the volume and clinical stage of prostate cancer [1,4]. Selective determination of the various forms of PSA (i.e. free PSA, PSA-ACT complex and total PSA) may be useful to distinguish BPH from untreated prostate cancer. The ratio of free PSA to PSA-ACT is lower in untreated prostate cancer patients than in normal individuals or in patients with BPH. Thus, in patients with PSA in the 4 to 10 mg/L range (diagnostic gray zone), determination of free PSA fractions as a percentage of the total levels (i.e. the free PSA percentage) has been proposed as a means to improve discrimination between BPH and untreated prostate cancer in patients [1,3,5,6].

It has been found that PSA is not specific to the prostate. There are a number of non-prostatic sources of PSA, such as the periurethral glands, breast cancer tissues, breast fluids, amniotic fluid and other [7]. Urinary PSA has also been measured in women with hyperandrogenic syndromes such as polycystic ovarian syndrome [8].

  1. Vessella RL. Clin Chem 39:2035, 1993.
  2. Papsidero LD, et al. Cancer Res 40:2428, 1980.
  3. Kuriyama M, et al. Cancer Res 40:4658, 1980.
  4. Graves HCB. Clin Chem 38:1930, 1992.
  5. Lilja H, et al. Clin Chem 37:1618, 1991.
  6. Stenman UH, et al. Cancer Res 51:222, 1991.
  7. Diamandis EP & Yu H. Urol Clin North Am 24:275-282, 1997.
  8. Obiezu CV et al. J Clin Endocrinol Metab 86:1558-1561, 2001.
Info Diagnostic Systems Laboratories, Inc.
Customer Service: (800) 231-7970
Web site:

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