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TPO (Ab) EIA Thyroid Function 012-MDKAM-1 Thyroid Peroxidase

ProductsTPO (Ab) EIA Thyroid Function 012-MDKAM-1 Thyroid Peroxidase
Company ALPCO Diagnostics
Item TPO (Ab) EIA Thyroid Function 012-MDKAM-1 Thyroid Peroxidase
Description The anti-TPO test kit is a solid phase enzyme immunometric assay (ELISA) in microplate format. It is designed for the quantitative measurement of autoantibodies directed against thyroid peroxidase (TPO) in serum or plasma. The microplate is coated with human thyroid-peroxidase. Phase 1: Calibrators, controls and patient samples are pipetted into the antigen coated microplate. During a 30 minutes incubation present autoantibodies in the sample bind to the antigens fixed on the inner surface of the wells. Non-reactive serum components are removed by a washing step. Phase 2: Afterwards, an anti-human-IgG antibody, which is labeled with horseradish peroxidase, is added. During a 15 minute incubation, the autoantibodies bound to the antigen are specifically recognized by the enzyme labeled antibodies, and a sandwich complex is formed. An excess of enzyme conjugate is washed out. Phase 3: A chromogenic substrate, TMB (93,3,5,5-Tetra-Methyl-Benzidine), is added to all wells. During a 15 minute incubation, the substrate is converted to a colored end product (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensity is directly proportional to the concentration of anti-TPO autoantibodies present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600-690 nm reference filter is recommended.
Info ALPCO DiagnosticsCall ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) USACanada1-866-466-7731

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