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TG (Ab) EIA Thyroid Function 012-MDKAT-1 Thyroglobulin anti-tg

ProductsTG (Ab) EIA Thyroid Function 012-MDKAT-1 Thyroglobulin anti-tg
Company ALPCO Diagnostics
Item TG (Ab) EIA Thyroid Function 012-MDKAT-1 Thyroglobulin anti-tg
Features 
Description The anti-TG test kit is a solid phase enzyme immunometric assay (ELISA) in microplate format. It is designed for the quantitative measurement of IgG autoantibodies directed against thyroglobulin (TG) in serum or plasma. The microplate is coated with human TG. Phase 1: Calibrators, controls and patient samples are pipetted into the antigen coated microplate. During a 30 minute incubation autoantibodies present in the sample bind to the antigens fixed on the inner surface of the wells. Non-reactive serum components are removed by a washing step. Phase 2: Afterwards, an anti-human-IgG antibody, which is labeled with horseradish peroxidase, is added. During a 15 minute incubation, the autoantibodies bound to the antigen are specifically recognized by the enzyme labeled antibodies, and a sandwich complex is formed. An excess of enzyme conjugate is washed out. Phase 3: A chromogenic substrate, TMB (3,3,5,5-Tetra-Methyl-Benzidine), is added to all wells. During a second 15 minute, the substrate is converted to a colored end product (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensity is directly proportional to the concentration of anti-TG autoantibodies present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600-690 nm reference filter is recommended.
Info ALPCO DiagnosticsCall ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) USACanada1-866-466-7731

Customer Service: (800) 592-5726
Web site: http://www.alpco.com

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