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Retinol Binding Protein EIA Vitamin Assays 030-6110 RBP Bone and Minearl Metabolism

ProductsRetinol Binding Protein EIA Vitamin Assays 030-6110 RBP Bone and Minearl Metabolism
Company ALPCO Diagnostics
Item Retinol Binding Protein EIA Vitamin Assays 030-6110 RBP Bone and Minearl Metabolism
Features 
Description Retinol binding Proetin (RBP) is small (21kD) transport protein for vitamin A which forms a complex with pre-albumin in blood but loses its affinity for prealbumin once the vitamin has been deliverd to the target cells. The free RBP molecule is then rapidly filtered at the glomerulus and catabolized in the renal tubules after resorption by the proximal tubular cells (like other small molecules e.g. -2 Microglobulin). In kidney disease with prevailing tubular changes these proteins are not reabsorbed and appear in the urine. This Enzyme-Linked Immunosorbent Assay (ELISA) serves the quantitative determination of the Retinol binding protein (RBP) from plasma, serum and urine. In a 1 hour incubation step, the RBP in the samples is bound to an available excess of polyclonal rabbit antibodies against RBP, which is immobilized to the surface of the microtitre plates. After a washing step, to remove all foreign substances, the quantification of bound RBP is carried out by adding an enzyme labeled antibody, which also binds to the RBP. The amount of converted substrate is directly proportional to the amount of bound RBP and can be determined photometrically at 450 nm (if extinction is out of range, measure at 410 nm).
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