Insulin Jumbo EIA Diabetes and Obesity Markers 008-10-1113-99
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Insulin Jumbo EIA Diabetes and Obesity Markers 008-10-1113-99
| Products | Insulin Jumbo EIA Diabetes and Obesity Markers 008-10-1113-99 |
| Company | ALPCO Diagnostics |
| Item | Insulin Jumbo EIA Diabetes and Obesity Markers 008-10-1113-99 |
| Features | |
| Description | The ALPCO Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the human insulin molecule. During incubation, insulin in the sample reacts with peroxidase-conjugated anti-insulin antibodies and anti-insulin antibodies bound to the microplate wells. A simple washing step removes unbound enzyme labeled antibody. The bound conjugate is detected by reaction with 3,3',5,5'-tetramethylbenzidine (TMB). The reaction is stopped by adding acid. The colorimetric endpoint is read spectrophotometrically.The ALPCO Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the human insulin molecule. During incubation, insulin in the sample reacts with peroxidase-conjugated anti-insulin antibodies and anti-insulin antibodies bound to the microplate wells. A simple washing step removes unbound enzyme labeled antibody. The bound conjugate is detected by reaction with 3,3',5,5'-tetramethylbenzidine (TMB). The reaction is stopped by adding acid. The colorimetric endpoint is read spectrophotometrically. |
| Info | ALPCO Diagnostics Customer Service: (800) 592-5726 Web site: http://www.alpco.com |
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