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IGF-1 EIA Endocrine Function 026-AC-27F1 Insulin Growth Factor

ProductsIGF-1 EIA Endocrine Function 026-AC-27F1 Insulin Growth Factor
Company ALPCO Diagnostics
Item IGF-1 EIA Endocrine Function 026-AC-27F1 Insulin Growth Factor
Features 
Description Insulin-like growth factor 1 (IGF-1) is a polypeptide of 70 amino acids (7650 daltons), and is one of a number of related insulin-like growth factors present in the circulation. The molecule shows approximately 50% sequence homology with proinsulin and has a number of biological activities similar to insulin. The peptide is growth hormone (GH) dependent to a high degree, but there is growing evidence of GH-independent secretion. IGF-1 has numerous growth-promoting effects, including mitogenic effects and the promotion of cartilage sulphation. It also mediates growth promoting actions of growth hormone on skeletal and other body fluids. Almost all (>95%) of serum IGF-1 circulates bound to specific IGF binding proteins, of which six classes (IGF-BPs 1-6) are now recognised. BP3 is thought to be the major binding protein of IGF-1, forming a ternary complex of 140 000 daltons with IGF-1 and an acid-labile sub-unit. The measurement of serum IGF-1 is of recognised value in children with growth disorders and in the diagnosis and monitoring of acromegaly. IGF-1 concentrations change with age, nutritional status, body composition and growth hormone secretion. A single basal IGF-1 determination is useful in the assessment of short stature in children and in nutritional support studies of acutely ill patients. For the diagnosis of acromegaly, a single IGF-1 determination is considered more reliable than a random GH determination. Patient samples are incubated briefly with a reagent to inactivate binding proteins, and then diluted for assay. In the OCTEIA IGF-1 kit a purified sheep polyclonal anti-IGF-1 is coated onto the inner surface of polystyrene microtitre wells (the solid phase or capture antibody). The pre-treated, diluted sample is then incubated, together with horseradish peroxidase labeled monoclonal anti-IGF-1, in antibody coated wells for 2 hours at room temperature. The wells are washed and a single component chromogenic substrate (a formulation of tetramethylbenzidine) is added to develop color. The absorbance of the stopped reaction mixture is read in a microtitre plate reader, color intensity developed being directly proportional to the amount of IGF-1 present in the sample.
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