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Free Estriol (E3) EIA Reproductive Markers 020-DR-1612

ProductsFree Estriol (E3) EIA Reproductive Markers 020-DR-1612
Company ALPCO Diagnostics
Item Free Estriol (E3) EIA Reproductive Markers 020-DR-1612
Features 
Description The Estriol (unconjugated) enzyme immunoassay (EIA) test kit is to be used for the in vitro determination of unconjugated estriol in human serum during the second half of pregnancy. The Estriol (unconjugated) procedure is an enzyme immunoassay, which is based on the principle of competitive binding. In this procedure, estriol is conjugated to the enzyme horseradish peroxidase. Serum containing estriol (i.e. standard or patient) is mixed with estriol HRP conjugate and added to the wells on a microtiter strip. Estriol antibody is already precoated in the microwells. After one hour, the free estriol horseradish peroxidase and unbound estriol are washed from the wells with buffer. Substrate is added to the antibody bound estriol horseradish peroxidase which is immobilized in the wells. After a brief incubation period a stopping reagent is added, which gives rise to a colored product which is measured spectrophotometrically utilizing either a microplate or microstrip reader.
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