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Dynal RELI™ SSO

ProductsDynal RELI™ SSO
Company Dynal Biotech, LLC
Item Dynal RELI™ SSO
Features 
  • AutoRELI 48 instrument
  • RELI-Scan Instrument
  • Pattern Matching Program
  • Novel and Innovative Probe Design
  • Flexible System
  • Easy to perform Gel-Free Assay
  • Single Assay Protocol
  • Low to Medium Resolution
Description The Dynal RELI™ SSO typing technique utilises a generic PCR amplification with biotinylated primers that flank the specific target region of an HLA locus. This is followed by hybridisation of the resulting amplicon to an array of Sequence Specific Oligonucleotide probes immobilised on a nylon membrane. The immobilisation of these probes is referred to as a Reverse Line blot and thus gives rise to the acronym 'RELI™'.

Under appropriate wash conditions, the probes remain bound only to their complementary sequence in the amplified DNA. The sensitivity of the system enables single nucleotide differences to be distinguished. The presence of the amplicon/probe complex is detected using a simple colorimetric reaction and appears as a blue precipitate.

Info Dynal Biotech, LLC
Customer Service: (800) 558-4511
Web site: http://www.dynalbiotech.com

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