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Dual Media Kit

ProductsDual Media Kit
Company Zymo Research Corporation
Item Dual Media Kit
Features 
Description The Dual Media Set, different from commonly used protein expression procedures using Luria Broth (LB) or other specially prepared medium, contains two media, EXPANSION BROTH (EB) and OVEREXPRESSION BROTH (OB). For cell expansion, E. coli cells are grown in EB, while the production of the recombinant protein is almost completely repressed. For high level protein expression, the expanded cell culture is then simply added into OB media. By using the Dual Media Set system, overexpression of diverse proteins can be reliably controlled and synchronized.

With our new Dual Media Set, you can now reliably express recombinant proteins in E. coli to the highest levels. Expand your culture in the EXPANSION BROTH (EB).

Transfer the cells to OVEREXPRESSION BROTH (OB) and grow overnight. This media set was designed to overcome the limitations of Luria Broth (LB) when used for protein expression. LB, while an excellent medium for normal E. coli manipulation, is not a good medium for repression of protein expression during cell expansion, nor is it ideal for protein expression when inducer is added.

Info Zymo Research Corporation
Customer Service: (888) 882-9682
Web site: http://www.zymoresearch.com/

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