Navigation Links
Dual Media Kit

ProductsDual Media Kit
Company Zymo Research Corporation
Item Dual Media Kit
Features 
Description The Dual Media Set, different from commonly used protein expression procedures using Luria Broth (LB) or other specially prepared medium, contains two media, EXPANSION BROTH (EB) and OVEREXPRESSION BROTH (OB). For cell expansion, E. coli cells are grown in EB, while the production of the recombinant protein is almost completely repressed. For high level protein expression, the expanded cell culture is then simply added into OB media. By using the Dual Media Set system, overexpression of diverse proteins can be reliably controlled and synchronized.

With our new Dual Media Set, you can now reliably express recombinant proteins in E. coli to the highest levels. Expand your culture in the EXPANSION BROTH (EB).

Transfer the cells to OVEREXPRESSION BROTH (OB) and grow overnight. This media set was designed to overcome the limitations of Luria Broth (LB) when used for protein expression. LB, while an excellent medium for normal E. coli manipulation, is not a good medium for repression of protein expression during cell expansion, nor is it ideal for protein expression when inducer is added.

Info Zymo Research Corporation
Customer Service: (888) 882-9682
Web site: http://www.zymoresearch.com/

Related medicine products :

1. Advance Stemmed Medial-Pivot Knee
2. Perclose ProGlide Suture-Mediated Closure
3. Closer S Suture-Mediated Closure
4. ProStar XL 8 Suture-Mediated Closure
5. ProStar XL 10 Suture-Mediated Closure
6. Acquidata Uromac Median Urodynamics System
7. Perclose A-T Suture-Mediated Closure
8. BinaxNow E.coli 0157 Rapid Media
10mm Tip to Bend, 0.3mm Y Tip; In Titanium....
Double ended with 0.5mm x 10mm and 1.0mm x 15mm blades; both blades gently curved; round serrated handle, In Titanium....
2.5x8mm Spatula Combined with 8mm Blade; in stainless steel....
0.5mm "K" hook. Rounded knurled handle; shaft angled 11mm from tip; overall length 120mm, in Titanium....
Medicine Products:
(Date:4/29/2016)... , ... April 29, 2016 , ... ... Spiritual Awakening , announces the addition Onnit brand Alpha BRAIN and New Mood ... of Onnit brain and mood optimization products to the store is just one ...
(Date:4/29/2016)... ... , ... On Tuesday, April 26, 2016 members of the HomeTown Health network, ... Nathan Deal on SB 258, the “Rural Health Care Relief” Bill. , The bill, ... credit to individuals and corporations which donate directly to a “rural hospital” in Georgia, ...
(Date:4/29/2016)... Torrance, CA (PRWEB) , ... April 29, 2016 ... ... physician advocating optimistic healthcare awareness and author of best seller "LOVE, MEDICINE and ... Talk Radio Monday, May 2, 2016 and podcasted thereafter . Dr. Bernie ...
(Date:4/29/2016)... (PRWEB) , ... April 29, 2016 , ... Conditions were ... at Cove Island Park on Sunday, with sunny skies, a light breeze and temperatures ... nearly $33,000. , The 5k Run and Walk and 1-mile walk were ...
(Date:4/29/2016)... ... April 29, 2016 , ... A ... born with severe congenital diaphragmatic hernia have better survival rates if surgery is ... hernia (CDH)—a condition where the diaphragm fails to form completely, letting abdominal organs ...
Breaking Medicine News(10 mins):
(Date:4/27/2016)... , April 27, 2016 Shire plc ... Jeff Poulton , Chief Financial Officer, will present at the ... Boston, MA on Wednesday, May 04, 2016, 10:00 ... will be available on the Presentations and Webcasts section of ... of the webcast will be available on this same website ...
(Date:4/27/2016)... 27, 2016 Oasmia Pharmaceutical ... of a new generation of drugs within human ... results for Paclical/Apealea in the Phase III study ... epithelial ovarian cancer. These preliminary results showed non-inferiority ... with carboplatin versus Taxol in combination with carboplatin. ...
(Date:4/27/2016)... , April 27, 2016 ... reach USD 2.06 billion by 2022, according to ... Increasing consumer awareness towards a healthy lifestyle is ... seven years.      (Logo: http://photos.prnewswire.com/prnh/20150105/723757 ... coupled with rising health treatment expenditure has urged ...
Breaking Medicine Technology: