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C-Peptide EIA Diabetes and Obesity Markers 008-10-1136-01

ProductsC-Peptide EIA Diabetes and Obesity Markers 008-10-1136-01
Company ALPCO Diagnostics
Item C-Peptide EIA Diabetes and Obesity Markers 008-10-1136-01
Features 
Description Qualitative and quantitative evaluation of pancreatic b-cell function is not only of use in the pre- and post-diagnostic study of the natural history of diabetes mellitus, but is also relevant in clinical practice as a guide to the correct choice of treatment. Peripheral insulin levels cannot be used to assess b-cell function because of the large and variable uptake from the portal circulation into the liver, and because insulin assays cannot distinguish endogenous from exogenous insulin1,3. Human C-peptide is a 31 amino acid chain with a molecular mass of approximately 3020 daltons. Within the pancreatic b-cell, proinsulin is cleaved into one molecule of C-peptide and one molecule of insulin. C-peptide is subsequently released into the circulation at concentrations equimolar to those of insulin. In contrast to insulin, C-peptide is only minimally extracted by the liver. Peripheral C-peptide concentrations therefore reflect the secretion of b-cells more accurately than insulin2,4. The ALPCO C-Peptide ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. During incubation, C-peptide in the sample reacts with peroxidase-conjugated anti-C-peptide antibodies and anti-C-peptide antibodies bound to microplate wells. A simple washing step removes unbound enzyme labeled antibody. The bound conjugate is detected by reaction with 3,3',5,5'-tetramethylbenzidine (TMB). The reaction is stopped by adding acid. The colorimetric endpoint is read spectrophotometrically.
Info ALPCO DiagnosticsCall ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) USACanada1-866-466-7731

Customer Service: (800) 592-5726
Web site: http://www.alpco.com

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... ARUP Laboratories is a national reference ... laboratory research and development. ARUP offers an ... unique medical tests in clinical and anatomic ... ARUP Laboratories' clients include more than half ...
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
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