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ALS, Total ELISA Test

ProductsALS, Total ELISA Test
Company Diagnostic Systems Laboratories, Inc.
Item ALS, Total ELISA Test
Features 
Description Acid-labile subunit (ALS) is a GH dependent protein which is produced in the liver as a 578 amino acid peptide with a predicted molecular weight of 63.3 kDa. IGFs in human serum are mostly confined to a ~150 KDa GH-dependent ternary protein complex consisting of IGFBP-3, a molecule of IGF-I or IGF-II, and this unique acid-labile subunit (ALS) [1-6]. The first description of ALS was based on the observation that acidification would result in dissociation of the 150 KDa complex into acid-stable IGF (~7.5 KDa) and IGFBP-3 (~50 KDa) components while neutralization of the acidified sample would no longer support formation of the 150 KDa complex [7,8]. The existence of ALS was later confirmed by the finding that serum-derived ALS could readily convert binary IGF/IGFBP-3 complexes to the 150 KDa form and that IGFBP-3 occupancy by IGF-I or IGF-II was a prerequisite for the complex assembly [9,10] in vivo. However, ALS may also be capable of IGF-independent interaction with IGFBP-3 and as such modulate the binding capacity of IGFBP-3 for the IGF peptides [11].

  1. Jones JI et al. Endocrin Rev 16:3-34, 1995
  2. Rosenfeld RG et al. Recent Prog Horm Res 46:99-163, 1990
  3. Shimesaki S et al. Prog Growth Factor Res 1:243-266, 1991
  4. Lamson C et al. Growth Factors 5:19-28, 1991
  5. Holly JMP et al. Growth Regul 4(suppl 1):20-30, 1994
  6. Cohen P et al. Curr Opin Pediatr 6:462-467, 1994
  7. Furlanetto RM J Clin Endocrinol Metab 51:12-19, 1980
  8. Hintz RL et al in Growth hormone and other biologically active peptides. Amsterdam: Excerpta Medica 1980;133-143
  9. Baxter RC Clin Endocrinol Metab 67:265-272, 1988
  10. Baxter RC et al. J Biol Chem 264:11843-11848, 1989
  11. Barreca A et al. J Clin Endocrinol Metab 80:1318-1324, 1995.
Info Diagnostic Systems Laboratories, Inc.
Customer Service: (800) 231-7970
Web site: http://www.dslabs.com

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